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RANk elisa kit :: Rat Receptor Activator Of Nuclear Factor Kappa B (RANk) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022886 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Receptor Activator Of Nuclear Factor Kappa B (RANk), ELISA Kit

 Also Known As   

Receptor Activator Of Nuclear Factor Kappa B (RANk) ELISA Kit

 Product Synonym Names    CD265; TNFRSF11A; TNFRSF11-A; ODFR; OFE; TRANCER; Osteoclast differentiation factor receptor; Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator
 Product Gene Name   

RANk elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    259710
 3D Structure    ModBase 3D Structure for O14788
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B (RANk).
No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B (RANk) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.060ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Receptor Activator Of Nuclear Factor Kappa B (RANk) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RANk elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RANk purchase    MBS2022886 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Receptor Activator Of Nuclear Factor Kappa B (RANk) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing RANk, with an estimated sensitivity of < 0.060ng/mL. The ELISA analytical biochemical technique of the MBS2022886 kit is based on RANk antibody-RANk antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RANk antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RANk. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for RANk elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Activator Of Nuclear Factor Kappa B (RANk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Activator Of Nuclear Factor Kappa B (RANk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Activator Of Nuclear Factor Kappa B (RANk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Activator Of Nuclear Factor Kappa B (RANk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for RANk elisa kit    Signal transduction; Metabolic pathway; Bone metabolism
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 Typical Testing Data/Standard Curve (for reference only) of RANk elisa kit    RANk elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022886. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RANk. It may not necessarily be applicable to this product.
 NCBI GI #    18143619
 NCBI GeneID    8600
 NCBI Accession #    BAB79694.1 [Other Products]
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 UniProt Primary Accession #    O14788 [Other Products]
 UniProt Secondary Accession #    O14723; Q96Q17; Q9P2Q3 [Other Products]
 UniProt Related Accession #    O14788 [Other Products]
 Molecular Weight    30,523 Da
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 NCBI Official Full Name    receptor activator of nuclear factor kappa B ligand 1
 NCBI Official Synonym Full Names    tumor necrosis factor (ligand) superfamily, member 11
 NCBI Official Symbol    TNFSF11 [Similar Products]
 NCBI Official Synonym Symbols   
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
[Similar Products]
 NCBI Protein Information    tumor necrosis factor ligand superfamily member 11
 UniProt Protein Name    Tumor necrosis factor ligand superfamily member 11
 UniProt Synonym Protein Names   
Osteoclast differentiation factor; ODF
 UniProt Gene Name    TNFSF11 [Similar Products]
 UniProt Synonym Gene Names    OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE [Similar Products]
 UniProt Entry Name    TNF11_HUMAN
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 NCBI Summary for RANk    This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
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 UniProt Comments for RANk    TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: extracellular space; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; osteoclast differentiation; activation of JNK activity; positive regulation of homotypic cell-cell adhesion; activation of NF-kappaB transcription factor; positive regulation of corticotropin-releasing hormone secretion; calcium ion homeostasis; monocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; organ morphogenesis; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of T cell activation; protein homooligomerization; bone resorption

Disease: Osteopetrosis, Autosomal Recessive 2
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 Research Articles on RANk    1. We found high osteoprotogerin concentrations, increased CIMT [ carotid artery intima media thickness ] and decreased FMD [flow-mediated vasodilation], in women with PCOS [polycystic ovary syndrome].
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RANk elisa kitPathways associated with RANk elisa kit
 Reference Product  PubMed Publications
 TNFRSF11B elisa kit  >2341 publications with RANk and TNFRSF11B
 TNFRSF11A elisa kit  >1537 publications with RANk and TNFRSF11A
 NFATC1 elisa kit  >368 publications with RANk and NFATC1
 FOS elisa kit  >219 publications with RANk and FOS
 PTH elisa kit  >209 publications with RANk and PTH
 IL17A elisa kit  >82 publications with RANk and IL17A
 STAT3 elisa kit  >31 publications with RANk and STAT3
 IL6 elisa kit  >29 publications with RANk and IL6
 IL11 elisa kit  >19 publications with RANk and IL11
 IL1B elisa kit  >1 publications with RANk and IL1B
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram
 Osteoblast Signaling Pathway antibodies  Osteoblast Signaling Pathway Diagram
 Osteoclast Signaling Pathway antibodies  Osteoclast Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
Diseases associated with RANk elisa kitOrgans/Tissues associated with RANk elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1564 publications with RANk and Necrosis
 Osteoporosis Antibodies  >1149 publications with RANk and Osteoporosis
 Inflammation Antibodies  >544 publications with RANk and Inflammation
 Osteolysis Antibodies  >355 publications with RANk and Osteolysis
 Cardiovascular Diseases Antibodies  >320 publications with RANk and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >242 publications with RANk and Breast Neoplasms
 Alveolar Bone Loss Antibodies  >157 publications with RANk and Alveolar Bone Loss
 Pain Antibodies  >132 publications with RANk and Pain
 Osteopetrosis Antibodies  >124 publications with RANk and Osteopetrosis
 Neoplasm Metastasis Antibodies  >117 publications with RANk and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >778 publications with RANk and Connective Tissue
 Brain Antibodies  >347 publications with RANk and Brain
 Liver Antibodies  >107 publications with RANk and Liver
 Lymph Node Antibodies  >90 publications with RANk and Lymph Node
 Pancreas Antibodies  >17 publications with RANk and Pancreas
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