Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow RANKL rightarrow Rat RANKL  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CD42b Antibody
 • Microtubule Associated Protein RP-EB Family, Member 1 (MAPRE... ELISA Kit
 • Sperm (AsAb IgG) ELISA Kit
 • Interleukin 12 Receptor (IL-12R) ELISA Kit
 • Nesfatin1 (Nes-1) (Nes-1 ) ELISA Kit
 • Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) ELISA Kit
 • Myosin (MYS) ELISA Kit
 • ACTH (1-39) Peptide
 • Cholecystokinin (CCK) ELISA Kit
 • HBcAg Antibody
 • Creatine phosphokinase (CPK) ELISA Kit
 • receptor tyrosine kinase-like orphan receptor 2 (ROR2) ELISA Kit
 • Hydroxy-itraconazole Chemical Reference
 • BMP-4 Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

RANKL elisa kit :: Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit

Scan QR to view Datasheet Catalog #    MBS725602
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Receptor Activator of Nuclear Factor Kb Ligand (RANKL), ELISA Kit

★Popular Item★
 Also Known As   

Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA Kit

 Product Gene Name   

RANKL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Competitive ELISA Manual Insert    Competitive ELISA PDF Manual
  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602642
Section Bar
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of RANKL. No significant cross-reactivity or interference between RANKL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RANKL and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/ml
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RANKL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RANKL purchase    MBS725602 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Receptor Activator of Nuclear Factor Kb Ligand (RANKL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing RANKL, with an estimated sensitivity of 0.1ng/ml. The ELISA analytical biochemical technique of the MBS725602 kit is based on RANKL antibody-RANKL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RANKL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RANKL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for RANKL elisa kit

   Intended Uses: This RANKL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RANKL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: RANKL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for RANKL. Standards or samples are then added to the microtiter plate wells and RANKL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of RANKL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for RANKL are added to each well to "sandwich" the RANKL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RANKL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for RANKL elisa kit    Immunology
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of RANKL elisa kit    RANKL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for RANKL. It may not necessarily be applicable to this product.
 NCBI GI #    2612922
 NCBI GeneID    8600
 NCBI Accession #    AAB86811.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    O14723; Q96Q17; Q9P2Q3 [Other Products]
 UniProt Related Accession #    O14788 [Other Products]
 Molecular Weight    30,523 Da
Table BarTOPTable Bar
 NCBI Official Full Name    RANKL
 NCBI Official Synonym Full Names    tumor necrosis factor (ligand) superfamily, member 11
 NCBI Official Symbol    TNFSF11 [Similar Products]
 NCBI Official Synonym Symbols   
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
[Similar Products]
 NCBI Protein Information    tumor necrosis factor ligand superfamily member 11; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand
 UniProt Protein Name    Tumor necrosis factor ligand superfamily member 11
 UniProt Synonym Protein Names   
Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD_antigen: CD254Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form
 UniProt Gene Name    TNFSF11 [Similar Products]
 UniProt Synonym Gene Names    OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE [Similar Products]
 UniProt Entry Name    TNF11_HUMAN
Section Bar
 NCBI Summary for RANKL    This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for RANKL    TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: extracellular space; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; osteoclast differentiation; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of protein kinase B signaling cascade; organ morphogenesis; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription factor activity; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell activation; protein homooligomerization; bone resorption

Disease: Osteopetrosis, Autosomal Recessive 2
Table BarTOPTable Bar
 Research Articles on RANKL    1. RANKL-induced osteoclastogenesis is inhibited by MGF-E8 exogenously added to human osteoclast precursors.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with RANKL elisa kitPathways associated with RANKL elisa kit
 Reference Product  PubMed Publications
 TNFRSF11B elisa kit  >2259 publications with RANKL and TNFRSF11B
 TNFRSF11A elisa kit  >1522 publications with RANKL and TNFRSF11A
 NFATC1 elisa kit  >337 publications with RANKL and NFATC1
 PTH elisa kit  >205 publications with RANKL and PTH
 FOS elisa kit  >204 publications with RANKL and FOS
 IL17A elisa kit  >77 publications with RANKL and IL17A
 STAT3 elisa kit  >31 publications with RANKL and STAT3
 IL6 elisa kit  >28 publications with RANKL and IL6
 IL11 elisa kit  >19 publications with RANKL and IL11
 IL1B elisa kit  >1 publications with RANKL and IL1B
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram
 Osteoblast Signaling Pathway antibodies  Osteoblast Signaling Pathway Diagram
 Osteoclast Signaling Pathway antibodies  Osteoclast Signaling Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
 Prolactin Signaling Pathway antibodies  Prolactin Signaling Pathway Diagram
Diseases associated with RANKL elisa kitOrgans/Tissues associated with RANKL elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1531 publications with RANKL and Necrosis
 Osteoporosis Antibodies  >1108 publications with RANKL and Osteoporosis
 Inflammation Antibodies  >518 publications with RANKL and Inflammation
 Osteolysis Antibodies  >343 publications with RANKL and Osteolysis
 Cardiovascular Diseases Antibodies  >310 publications with RANKL and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >228 publications with RANKL and Breast Neoplasms
 Alveolar Bone Loss Antibodies  >147 publications with RANKL and Alveolar Bone Loss
 Pain Antibodies  >126 publications with RANKL and Pain
 Osteopetrosis Antibodies  >121 publications with RANKL and Osteopetrosis
 Neoplasm Metastasis Antibodies  >112 publications with RANKL and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >749 publications with RANKL and Connective Tissue
 Brain Antibodies  >343 publications with RANKL and Brain
 Liver Antibodies  >102 publications with RANKL and Liver
 Lymph Node Antibodies  >83 publications with RANKL and Lymph Node
 Pancreas Antibodies  >17 publications with RANKL and Pancreas
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions