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REG3b elisa kit :: Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024933 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
 
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 Product Name   

Regenerating Islet Derived Protein 3 Beta (REG3b), ELISA Kit

★Popular Item★
 Also Known As   

Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit

 Product Synonym Names    PAP1; Pancreatitis-Associated Protein 1
 Product Gene Name   

REG3b elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    167805
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Beta (REG3b).
No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Beta (REG3b) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.065ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Beta (REG3b) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Beta (REG3b) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REG3b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REG3b purchase    MBS2024933 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing REG3b, with an estimated sensitivity of < 0.065ng/mL. The ELISA analytical biochemical technique of the MBS2024933 kit is based on REG3b antibody-REG3b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REG3b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REG3b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for REG3b elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Beta (REG3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Beta (REG3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 3 Beta (REG3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for REG3b elisa kit    Human gene deletion
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 Typical Testing Data/Standard Curve of REG3b elisa kit    REG3b elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS2024933. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REG3b. It may not necessarily be applicable to this product.
 NCBI GI #    4505605
 NCBI GeneID    5068
 NCBI Accession #    NP_002571.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002580.2 [Other Products]
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 UniProt Related Accession #    Q06141 [Other Products]
 Molecular Weight    19,395 Da
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 NCBI Official Full Name    regenerating islet-derived protein 3-alpha
 NCBI Official Synonym Full Names    regenerating islet-derived 3 alpha
 NCBI Official Symbol    REG3A [Similar Products]
 NCBI Official Synonym Symbols   
HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
[Similar Products]
 NCBI Protein Information    regenerating islet-derived protein 3-alpha
 UniProt Protein Name    Regenerating islet-derived protein 3-alpha
 UniProt Synonym Protein Names   
Hepatointestinal pancreatic protein; HIP/PAP
 Protein Family    Regenerating islet-derived protein
 UniProt Gene Name    REG3A [Similar Products]
 UniProt Synonym Gene Names    HIP; PAP; PAP1; REG-3-alpha; HIP/PAP; Reg III-alpha [Similar Products]
 UniProt Entry Name    REG3A_HUMAN
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 NCBI Summary for REG3b    This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]
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 UniProt Comments for REG3b    REG3A: Might be a stress protein involved in the control of bacterial proliferation.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: extracellular space; cytoplasm

Molecular Function: carbohydrate binding

Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response
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 Research Articles on REG3b    1. Reg3A overexpression promoted cell growth in pancreatic cancer. SOCS3 is a key target in cancer by inhibiting cell growth and inducing apoptosis. SOCS3 negatively regulated Reg3A-mediated cell growth in pancreatic cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with REG3b elisa kitDiseases associated with REG3b elisa kit
 Reference Product  PubMed Publications
 REG1A elisa kit  >6 publications with REG3b and REG1A
 FN1 elisa kit  >1 publications with REG3b and FN1
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with REG3b and Inflammation
 Carcinoma Antibodies  >7 publications with REG3b and Carcinoma
 Adenocarcinoma Antibodies  >5 publications with REG3b and Adenocarcinoma
 Pancreatic Neoplasms Antibodies  >5 publications with REG3b and Pancreatic Neoplasms
 Liver Neoplasms Antibodies  >3 publications with REG3b and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3 publications with REG3b and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >2 publications with REG3b and Disease Models, Animal
 Insulin Resistance Antibodies  >1 publications with REG3b and Insulin Resistance
 Obesity Antibodies  >1 publications with REG3b and Obesity
 Colonic Neoplasms Antibodies  >1 publications with REG3b and Colonic Neoplasms
Organs/Tissues associated with REG3b elisa kit
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >13 publications with REG3b and Pancreas
 Liver Antibodies  >5 publications with REG3b and Liver
 Intestine Antibodies  >5 publications with REG3b and Intestine
 Stomach Antibodies  >2 publications with REG3b and Stomach
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