Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow SNAP-25 rightarrow Rat SNAP-25  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ISG15 Antibody
 • Activation-induced cytidine deaminase (AICDA) ELISA Kit
 • Anti-Glutamic Acid Decarboxylase Antibodies (GAD) ELISA Kit
 • Mercaptopyruvate Sulfurtransferase (MPST) ELISA Kit
 • Pleural Fluid Human Fluid
 • ApoA-I Antibody
 • coagulation factor XIII, A1 polypeptide (F13A1) ELISA Kit
 • Dopamine D4 Receptor (a.a. 176-185) (DRD4) Antibody
 • Selenoprotein P1, Plasma (SEPP1) Recombinant Protein
 • Vimentin Antibody
 • cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP1... ELISA Kit
 • Solute carrier family 35 member F2, SLC35F2 ELISA Kit
 • PRKCH Antibody
 • LIMK1 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

SNAP-25 elisa kit :: Rat SNAP-25 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2514285
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SNAP-25, ELISA Kit

★Popular Item★
 Also Known As   

Rat SNAP-25(Synaptosome Associated Protein 25)ELISA Kit

 Product Gene Name   

SNAP-25 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600322
Section Bar
 Species Reactivity    Rat
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat SNAP-25. No significant cross-reactivity or interference between Rat SNAP-25 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat SNAP-25 and all the analogues, therefore, cross reaction may still exist.
Section Bar
 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
Section Bar
 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SNAP-25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SNAP-25 purchase    MBS2514285 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SNAP-25, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing SNAP-25, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2514285 kit is based on SNAP-25 antibody-SNAP-25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SNAP-25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SNAP-25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for SNAP-25 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SNAP-25 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SNAP-25. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat SNAP-25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SNAP-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SNAP-25. You can calculate the concentration of Rat SNAP-25 in the samples by comparing the OD of the samples to the standard curve.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of SNAP-25 elisa kit    SNAP-25 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2514285. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SNAP-25. It may not necessarily be applicable to this product.
 NCBI GI #    46397726
 NCBI GeneID    6616
 NCBI Accession #    P60880.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17 [Other Products]
 UniProt Related Accession #    P60880 [Other Products]
 Molecular Weight    23,336 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Synaptosomal-associated protein 25
 NCBI Official Synonym Full Names    synaptosomal-associated protein, 25kDa
 NCBI Official Symbol    SNAP25 [Similar Products]
 NCBI Official Synonym Symbols   
RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
[Similar Products]
 NCBI Protein Information    synaptosomal-associated protein 25; SUP; resistance to inhibitors of cholinesterase 4 homolog; super protein; synaptosomal-associated protein, 25kD
 UniProt Protein Name    Synaptosomal-associated protein 25
 UniProt Synonym Protein Names   
Super protein; SUP; Synaptosomal-associated 25 kDa protein
 UniProt Gene Name    SNAP25 [Similar Products]
 UniProt Synonym Gene Names    SNAP; SNAP-25; SUP [Similar Products]
 UniProt Entry Name    SNP25_HUMAN
Section Bar
 NCBI Summary for SNAP-25    Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for SNAP-25    SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; regulation of insulin secretion; synaptic vesicle priming; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18
Table BarTOPTable Bar
 Research Articles on SNAP-25    1. Full-length SNAP25 is cleaved more efficiently by the protease domain of serotype A botulinum neurotoxin than its shorter fragments.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with SNAP-25 elisa kitDiseases associated with SNAP-25 elisa kit
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Dopamine Neurotransmitter Release Cycle Pathway antibodies  Dopamine Neurotransmitter Release Cycle Pathway Diagram
 Effects Of Botulinum Toxin Pathway antibodies  Effects Of Botulinum Toxin Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
 Glutamate Neurotransmitter Release Cycle Pathway antibodies  Glutamate Neurotransmitter Release Cycle Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Secretion Pathway antibodies  Insulin Secretion Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >127 publications with SNAP-25 and Nervous System Diseases
 Paralysis Antibodies  >22 publications with SNAP-25 and Paralysis
 Cognition Disorders Antibodies  >21 publications with SNAP-25 and Cognition Disorders
 Memory Disorders Antibodies  >20 publications with SNAP-25 and Memory Disorders
 Seizures Antibodies  >17 publications with SNAP-25 and Seizures
 Pain Antibodies  >17 publications with SNAP-25 and Pain
 Learning Disorders Antibodies  >15 publications with SNAP-25 and Learning Disorders
 Neurobehavioral Manifestations Antibodies  >14 publications with SNAP-25 and Neurobehavioral Manifestations
 Movement Disorders Antibodies  >14 publications with SNAP-25 and Movement Disorders
 Diabetes Mellitus Antibodies  >11 publications with SNAP-25 and Diabetes Mellitus
Organs/Tissues associated with SNAP-25 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >668 publications with SNAP-25 and Nerve
 Brain Antibodies  >448 publications with SNAP-25 and Brain
 Pancreas Antibodies  >53 publications with SNAP-25 and Pancreas
 Muscle Antibodies  >46 publications with SNAP-25 and Muscle
 Embryonic Tissue Antibodies  >45 publications with SNAP-25 and Embryonic Tissue
 Eye Antibodies  >37 publications with SNAP-25 and Eye
 Ganglia Antibodies  >30 publications with SNAP-25 and Ganglia
 Pituitary Gland Antibodies  >22 publications with SNAP-25 and Pituitary Gland
 Kidney Antibodies  >17 publications with SNAP-25 and Kidney
 Adrenal Gland Antibodies  >15 publications with SNAP-25 and Adrenal Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions