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Pon3 elisa kit :: Rat Serum paraoxonase/lactonase 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2881619
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Serum paraoxonase/lactonase 3 (Pon3), ELISA Kit

★Popular Item★
 Also Known As   

Rat Serum paraoxonase/lactonase 3 ELISA Kit

 Product Synonym Names    Serum paraoxonase/lactonase 3; Pon3;
 Product Gene Name   

Pon3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2881619 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q68FP2
 Species Reactivity    Rat
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 Specificity    Recombinant and natural Rat Serum paraoxonase/lactonase 3
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 Assay Type    Sandwich
 Detection Range    0.312-20 ng/mL
 Sensitivity    0.162 ng/mL
 Intra-Assay CV    <=4.9%
 Inter-Assay CV    <=6.2%
 Recovery    86%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Pon3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Pon3 purchase    MBS2881619 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serum paraoxonase/lactonase 3 (Pon3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20 ng/mL in biological research samples containing Pon3, with an estimated sensitivity of 0.162 ng/mL. The ELISA analytical biochemical technique of the MBS2881619 kit is based on Pon3 antibody-Pon3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pon3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pon3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Pon3 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Pon3 elisa kit    Pon3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2881619. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Pon3. It may not necessarily be applicable to this product.
 NCBI GI #    51854237
 NCBI GeneID    312086
 NCBI Accession #    NP_001004086.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001004086.1 [Other Products]
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 UniProt Primary Accession #    Q68FP2 [Other Products]
 UniProt Related Accession #    Q68FP2 [Other Products]
 Molecular Weight    39,458 Da
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 NCBI Official Full Name    serum paraoxonase/lactonase 3
 NCBI Official Synonym Full Names    paraoxonase 3
 NCBI Official Symbol    Pon3 [Similar Products]
 NCBI Protein Information    serum paraoxonase/lactonase 3
 UniProt Protein Name    Serum paraoxonase/lactonase 3
 Protein Family    Serum paraoxonase/lactonase
 UniProt Gene Name    Pon3 [Similar Products]
 UniProt Entry Name    PON3_RAT
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 NCBI Summary for Pon3    microsomal paraoxonase that has esterase activity on a broad range of substrates [RGD, Feb 2006]
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 UniProt Comments for Pon3    Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents ().
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 Research Articles on Pon3    1. Study documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Pon3 elisa kitPathways associated with Pon3 elisa kit
 Reference Product  PubMed Publications
 Apoa1 elisa kit  >2 publications with Pon3 and Apoa1
 Asb4 elisa kit  >1 publications with Pon3 and Asb4
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Phase I, Non P450 Pathway antibodies  Phase I, Non P450 Pathway Diagram
Diseases associated with Pon3 elisa kitOrgans/Tissues associated with Pon3 elisa kit
 Disease Name  Pubmed Publications
 Atherosclerosis Antibodies  >54 publications with Pon3 and Atherosclerosis
 Heart Diseases Antibodies  >20 publications with Pon3 and Heart Diseases
 Inflammation Antibodies  >15 publications with Pon3 and Inflammation
 Liver Diseases Antibodies  >10 publications with Pon3 and Liver Diseases
 Fatty Liver Antibodies  >6 publications with Pon3 and Fatty Liver
 Poisoning Antibodies  >4 publications with Pon3 and Poisoning
 Kidney Diseases Antibodies  >3 publications with Pon3 and Kidney Diseases
 Necrosis Antibodies  >3 publications with Pon3 and Necrosis
 Carcinoma, Hepatocellular Antibodies  >2 publications with Pon3 and Carcinoma, Hepatocellular
 Fibrosis Antibodies  >2 publications with Pon3 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >43 publications with Pon3 and Liver
 Lung Antibodies  >11 publications with Pon3 and Lung
 Adipose Tissue Antibodies  >2 publications with Pon3 and Adipose Tissue
 Eye Antibodies  >2 publications with Pon3 and Eye
 Small Intestine Antibodies  >1 publications with Pon3 and Small Intestine
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