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Stat3 elisa kit :: Rat Signal transducer and activator of transcription 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760293
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
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 Product Name   

Signal transducer and activator of transcription 3 (Stat3), ELISA Kit

★Popular Item★
 Also Known As   

Rat Signal transducer and activator of transcription 3 ELISA Kit

 Product Synonym Names    Stat3/Signal transducer and activator of transcription 3
 Product Gene Name   

Stat3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760293 COA    COA PDF
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Stat3 . No significant cross-reactivity or interference between Stat3 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between Stat3 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/ml
 Sensitivity    <0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stat3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stat3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Stat3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Stat3 purchase    MBS760293 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Signal transducer and activator of transcription 3 (Stat3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing Stat3, with an estimated sensitivity of <0.094ng/ml. The ELISA analytical biochemical technique of the MBS760293 kit is based on Stat3 antibody-Stat3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Stat3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Stat3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Stat3 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Stat3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Stat3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Stat3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Stat3 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of Stat3 elisa kit    Stat3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760293. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Stat3. It may not necessarily be applicable to this product.
 NCBI GI #    6981592
 NCBI GeneID    25125
 NCBI Accession #    NP_036879.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012747.2 [Other Products]
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 UniProt Related Accession #    P52631 [Other Products]
 Molecular Weight    88,040 Da
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 NCBI Official Full Name    signal transducer and activator of transcription 3
 NCBI Official Synonym Full Names    signal transducer and activator of transcription 3 (acute-phase response factor)
 NCBI Official Symbol    Stat3 [Similar Products]
 NCBI Protein Information    signal transducer and activator of transcription 3
 UniProt Protein Name    Signal transducer and activator of transcription 3
 Protein Family    Signal transducer and activator of transcription
 UniProt Gene Name    Stat3 [Similar Products]
 UniProt Entry Name    STAT3_RAT
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 NCBI Summary for Stat3    transcription factor that plays a role in induction of gene expression during acute phase response [RGD, Feb 2006]
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 UniProt Comments for Stat3    STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; DNA-binding

Cellular Component: cytoplasm; cytosol; mitochondrial inner membrane; mitochondrion; nuclear chromatin; nucleoplasm; nucleus; plasma membrane

Molecular Function: CCR5 chemokine receptor binding; chromatin DNA binding; DNA binding; glucocorticoid receptor binding; identical protein binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; protein kinase binding; protein phosphatase binding; sequence-specific DNA binding; signal transducer activity; transcription factor activity; transcription factor binding

Biological Process: acute-phase response; aging; astrocyte differentiation; cell proliferation; cellular response to hormone stimulus; eating behavior; eye photoreceptor cell differentiation; glucose homeostasis; intracellular receptor-mediated signaling pathway; JAK-STAT cascade; leptin-mediated signaling pathway; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of glycolysis; phosphorylation; positive regulation of cell proliferation; positive regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus; radial glial cell differentiation; regulation of cell cycle; regulation of mitochondrial membrane permeability; regulation of multicellular organism growth; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to organic cyclic substance; response to organic substance; response to peptide hormone stimulus; sexual reproduction; stem cell maintenance; thermoregulation; transcription from RNA polymerase II promoter
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 Research Articles on Stat3    1. tunicamycin treatment completely replaced glycosylated gp130 with its unglycosylated form and inhibited STAT3 and ERK1/2 activation by IL-6 family cytokines in cultured cardiac myocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Stat3 elisa kitPathways associated with Stat3 elisa kit
 Reference Product  PubMed Publications
 Stat1 elisa kit  >1870 publications with Stat3 and Stat1
 Jak2 elisa kit  >1662 publications with Stat3 and Jak2
 Src elisa kit  >881 publications with Stat3 and Src
 Socs3 elisa kit  >806 publications with Stat3 and Socs3
 Jak1 elisa kit  >671 publications with Stat3 and Jak1
 Egfr elisa kit  >525 publications with Stat3 and Egfr
 Tyk2 elisa kit  >249 publications with Stat3 and Tyk2
 Jak3 elisa kit  >238 publications with Stat3 and Jak3
 Lck elisa kit  >28 publications with Stat3 and Lck
 Yes1 elisa kit  >1 publications with Stat3 and Yes1
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 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
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 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
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 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
Diseases associated with Stat3 elisa kitOrgans/Tissues associated with Stat3 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2249 publications with Stat3 and Inflammation
 Carcinoma Antibodies  >1843 publications with Stat3 and Carcinoma
 Necrosis Antibodies  >947 publications with Stat3 and Necrosis
 Adenocarcinoma Antibodies  >824 publications with Stat3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >597 publications with Stat3 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >537 publications with Stat3 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >507 publications with Stat3 and Liver Neoplasms
 Autoimmune Diseases Antibodies  >494 publications with Stat3 and Autoimmune Diseases
 Neoplasm Metastasis Antibodies  >413 publications with Stat3 and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >366 publications with Stat3 and Carcinoma, Squamous Cell
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1551 publications with Stat3 and Liver
 Brain Antibodies  >1240 publications with Stat3 and Brain
 Lung Antibodies  >1027 publications with Stat3 and Lung
 Muscle Antibodies  >914 publications with Stat3 and Muscle
 Heart Antibodies  >676 publications with Stat3 and Heart
 Colon Antibodies  >447 publications with Stat3 and Colon
 Kidney Antibodies  >436 publications with Stat3 and Kidney
 Prostate Antibodies  >399 publications with Stat3 and Prostate
 Nerve Antibodies  >390 publications with Stat3 and Nerve
 Adipose Tissue Antibodies  >255 publications with Stat3 and Adipose Tissue
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