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SGLT2 elisa kit :: Rat Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022406 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $530 +1 FREE 8GB USB
96-Strip-Wells  /  $715 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
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 Product Name   

Sodium/Glucose Cotransporter 2 (SGLT2), ELISA Kit

★Popular Item★
 Also Known As   

Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit

 Product Synonym Names    SLC5A2; Solute Carrier Family 5 Member 2; Low affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 2
 Product Gene Name   

SGLT2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    182381
 3D Structure    ModBase 3D Structure for P31639
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Sodium/Glucose Cotransporter 2 (SGLT2).
No significant cross-reactivity or interference between Sodium/Glucose Cotransporter 2 (SGLT2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.781-50ng/mL
 Sensitivity    < 0.28ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Sodium/Glucose Cotransporter 2 (SGLT2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SGLT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SGLT2 purchase    MBS2022406 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.781-50ng/mL in biological research samples containing SGLT2, with an estimated sensitivity of < 0.28ng/mL. The ELISA analytical biochemical technique of the MBS2022406 kit is based on SGLT2 antibody-SGLT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SGLT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SGLT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SGLT2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Glucose Cotransporter 2 (SGLT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Glucose Cotransporter 2 (SGLT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Glucose Cotransporter 2 (SGLT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for SGLT2 elisa kit    Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of SGLT2 elisa kit    SGLT2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022406. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SGLT2. It may not necessarily be applicable to this product.
 NCBI GI #    4507033
 NCBI GeneID    6524
 NCBI Accession #    NP_003032.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_003041.3 [Other Products]
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 UniProt Primary Accession #    P31639 [Other Products]
 UniProt Secondary Accession #    A2RRD2 [Other Products]
 UniProt Related Accession #    P31639 [Other Products]
 Molecular Weight    49,357 Da
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 NCBI Official Full Name    sodium/glucose cotransporter 2
 NCBI Official Synonym Full Names    solute carrier family 5 (sodium/glucose cotransporter), member 2
 NCBI Official Symbol    SLC5A2 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    sodium/glucose cotransporter 2
 UniProt Protein Name    Sodium/glucose cotransporter 2
 UniProt Synonym Protein Names   
Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2
 UniProt Gene Name    SLC5A2 [Similar Products]
 UniProt Synonym Gene Names    SGLT2; Na(+)/glucose cotransporter 2 [Similar Products]
 UniProt Entry Name    SC5A2_HUMAN
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 NCBI Summary for SGLT2    This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015]
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 UniProt Comments for SGLT2    SLC5A2: Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1. Defects in SLC5A2 are the cause of renal glucosuria (GLYS1). GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: low-affinity glucose:sodium symporter activity

Biological Process: transport; sodium ion transport; carbohydrate metabolic process; glucose transport; transmembrane transport

Disease: Renal Glucosuria
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 Research Articles on SGLT2    1. SGLT2 is inhibited with dapagliflozin in pancreatic alpha cells, which triggers glucagon secretion
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SGLT2 elisa kitPathways associated with SGLT2 elisa kit
 Reference Product  PubMed Publications
 SLC2A2 elisa kit  >2 publications with SGLT2 and SLC2A2
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Hexose Transport Pathway antibodies  Hexose Transport Pathway Diagram
 Inositol Transporters Pathway antibodies  Inositol Transporters Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Myoclonic Epilepsy Of Lafora Pathway antibodies  Myoclonic Epilepsy Of Lafora Pathway Diagram
 Na+-dependent Glucose Transporters Pathway antibodies  Na+-dependent Glucose Transporters Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
Diseases associated with SGLT2 elisa kitOrgans/Tissues associated with SGLT2 elisa kit
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >121 publications with SGLT2 and Diabetes Mellitus, Type 2
 Glycosuria Antibodies  >51 publications with SGLT2 and Glycosuria
 Glycosuria, Renal Antibodies  >42 publications with SGLT2 and Glycosuria, Renal
 Kidney Diseases Antibodies  >38 publications with SGLT2 and Kidney Diseases
 Weight Loss Antibodies  >17 publications with SGLT2 and Weight Loss
 Weight Gain Antibodies  >10 publications with SGLT2 and Weight Gain
 Cardiovascular Diseases Antibodies  >8 publications with SGLT2 and Cardiovascular Diseases
 Inflammation Antibodies  >7 publications with SGLT2 and Inflammation
 Hypertension Antibodies  >6 publications with SGLT2 and Hypertension
 Death Antibodies  >5 publications with SGLT2 and Death
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >96 publications with SGLT2 and Kidney
 Pancreas Antibodies  >8 publications with SGLT2 and Pancreas
 Intestine Antibodies  >5 publications with SGLT2 and Intestine
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