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Sp1 elisa kit :: Rat Sp1 transcription factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2603013
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Sp1 transcription factor, ELISA Kit

★Popular Item★
 Also Known As   

Rat Transcription factor Sp1, SP1 ELISA Kit

 Product Gene Name   

Sp1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q01714
 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or cell culture supernatant
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/mL
 Intra-assay Precision    ? 8%
 Inter-assay Precision    ? 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Sp1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Sp1 purchase    MBS2603013 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sp1 transcription factor, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing Sp1, with an estimated sensitivity of 0.06 ng/mL. The ELISA analytical biochemical technique of the MBS2603013 kit is based on Sp1 antibody-Sp1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sp1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sp1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Sp1 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat SP1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of Sp1 elisa kit    Sp1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2603013. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Sp1. It may not necessarily be applicable to this product.
 NCBI GI #    164414419
 NCBI GeneID    24790
 NCBI Accession #    NP_036787.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012655.2 [Other Products]
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 UniProt Primary Accession #    Q01714 [Other Products]
 UniProt Secondary Accession #    Q8K4R0 [Other Products]
 UniProt Related Accession #    Q01714 [Other Products]
 Molecular Weight    80,772 Da [Similar Products]
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 NCBI Official Full Name    transcription factor Sp1
 NCBI Official Synonym Full Names    Sp1 transcription factor
 NCBI Official Symbol    Sp1 [Similar Products]
 NCBI Protein Information    transcription factor Sp1; trans-acting transcription factor 1
 UniProt Protein Name    Transcription factor Sp1
 Protein Family    Sex-specific storage-protein
 UniProt Gene Name    Sp1 [Similar Products]
 UniProt Entry Name    SP1_RAT
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 NCBI Summary for Sp1    transcription factor that recognizes 5'-CCGCCC promoter sequence; plays a role in transcriptional regulation of many genes [RGD, Feb 2006]
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 UniProt Comments for Sp1    SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; nucleus

Molecular Function: protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; ossification; in utero embryonic development; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; rhythmic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; lung development
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 Research Articles on Sp1    1. Manganese treatment induced robust Sp1 phosphorylation and increased its DNA binding activity. Overexpression of mutant Sp1 lacking phosphorylation sites attenuated Sp1's ability to stimulate manganese-induced caspase-3 promoter activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Sp1 elisa kitDiseases associated with Sp1 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Mitochondrial Gene Expression Pathway antibodies  Mitochondrial Gene Expression Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Oxidative Stress Pathway antibodies  Oxidative Stress Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Selenium Metabolism Selenoproteins Pathway antibodies  Selenium Metabolism Selenoproteins Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1685 publications with Sp1 and Neoplasms
 Carcinoma Antibodies  >715 publications with Sp1 and Carcinoma
 Adenocarcinoma Antibodies  >370 publications with Sp1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >291 publications with Sp1 and Breast Neoplasms
 Leukemia Antibodies  >260 publications with Sp1 and Leukemia
 Nervous System Diseases Antibodies  >243 publications with Sp1 and Nervous System Diseases
 Necrosis Antibodies  >238 publications with Sp1 and Necrosis
 Liver Diseases Antibodies  >236 publications with Sp1 and Liver Diseases
 Liver Neoplasms Antibodies  >189 publications with Sp1 and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >175 publications with Sp1 and Cardiovascular Diseases
Organs/Tissues associated with Sp1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >673 publications with Sp1 and Liver
 Lung Antibodies  >373 publications with Sp1 and Lung
 Heart Antibodies  >262 publications with Sp1 and Heart
 Embryonic Tissue Antibodies  >258 publications with Sp1 and Embryonic Tissue
 Placenta Antibodies  >255 publications with Sp1 and Placenta
 Colon Antibodies  >157 publications with Sp1 and Colon
 Prostate Antibodies  >153 publications with Sp1 and Prostate
 Ovary Antibodies  >124 publications with Sp1 and Ovary
 Eye Antibodies  >94 publications with Sp1 and Eye
 Connective Tissue Antibodies  >80 publications with Sp1 and Connective Tissue
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