|
| Product Name
|
|
Steroid 5 Alpha Reductase 2 (SRD5a2), ELISA Kit ★Popular Item★
|
| Also Known As
|
|
Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit
|
| Product Synonym Names
|
|
3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
| Sample Manual Insert
|
|
 
|
| Request for Current Manual Insert
|
|
 |
|
| OMIM
|
|
264600
|
| 3D Structure
|
|
ModBase 3D Structure for P31213
|
|
| Species Reactivity
|
|
Rat
|
|
|
| Specificity
|
|
This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2).
No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) and analogues was observed.
|
|
|
| Assay Type
|
|
Double-antibody Sandwich
|
| Samples
|
|
Serum, Plasma, Tissue homogenates and Other Biological Fluids
|
| Detection Range
|
|
0.156-10ng/mL
|
| Sensitivity
|
|
< 0.065ng/mL
|
| Application
|
|
Enzyme-linked immunosorbent assay for Antigen Detection.
|
| Intra-assay Precision (Precision within an assay)
|
|
3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested 20 times on one plate, respectively.
|
| Inter-assay Precision (Precision between assays)
|
|
3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested on 3 different plates, 8 replicates in each plate.
|
| CV(%) =
|
|
SD/meanX100
|
| Intra-Assay
|
|
CV<10%
|
| Inter-Assay
|
|
CV<12%
|
|
|
| Preparation and Storage
|
|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|
| Product Note
|
|
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
|
| Other Notes
|
|
Small volumes of SRD5a2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
| Searchable Terms for SRD5a2 purchase
|
|
MBS2023257 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing SRD5a2, with an estimated sensitivity of < 0.065ng/mL. The ELISA analytical biochemical technique of the MBS2023257 kit is based on SRD5a2 antibody-SRD5a2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRD5a2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRD5a2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
|
|
TOP
|
|
|
|
|
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 2 (SRD5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
|
| Product Categories/Family for SRD5a2 elisa kit
|
|
Enzyme & Kinase
|
|
|
|
| Typical Standard Curve/Testing Data of SRD5a2 elisa kit
|
|
|
|
TOP
|
|
| Sample Manual Insert of MBS2023257. Click to request current manual
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for SRD5a2. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
338469
|
| NCBI GeneID
|
|
6716
|
| NCBI Accession #
|
|
AAA60586.1
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
P31213
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
Q2M1R4; Q9BYE6; B2RE87
|
[Other Products]
|
| UniProt Related Accession #
|
|
P31213
|
[Other Products]
|
| Molecular Weight
|
|
28,393 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
steroid 5-alpha-reductase 2
|
| NCBI Official Synonym Full Names
|
|
steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
|
| NCBI Official Symbol
|
|
SRD5A2
|
[Similar Products]
|
| NCBI Protein Information
|
|
3-oxo-5-alpha-steroid 4-dehydrogenase 2
|
| UniProt Protein Name
|
|
3-oxo-5-alpha-steroid 4-dehydrogenase 2
|
| UniProt Synonym Protein Names
|
|
5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase
|
| Protein Family
|
|
3-oxo-5-alpha-steroid 4-dehydrogenase
|
| UniProt Gene Name
|
|
SRD5A2
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
S5AR 2
|
[Similar Products]
|
| UniProt Entry Name
|
|
S5A2_HUMAN
|
|
|
| NCBI Summary for SRD5a2
|
|
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]
|
|
|
| UniProt Comments for SRD5a2
|
|
SRD5A2: Converts testosterone (T) into 5-alpha- dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH). A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. Belongs to the steroid 5-alpha reductase family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.3.1.22; Lipid Metabolism - androgen and estrogen
Chromosomal Location of Human Ortholog: 2p23
Cellular Component: endoplasmic reticulum membrane; cell soma; integral to membrane
Molecular Function: amide binding; cholestenone 5-alpha-reductase activity; sterol 5-alpha reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity
Biological Process: steroid metabolic process; response to drug; response to nutrient levels; response to peptide hormone stimulus; hypothalamus development; dibenzo-p-dioxin metabolic process; phthalate metabolic process; androgen metabolic process; male gonad development; hippocampus development; androgen biosynthetic process; response to testosterone stimulus; female genitalia development; cell-cell signaling; biphenyl metabolic process; male genitalia development; cell differentiation; response to follicle-stimulating hormone stimulus; steroid catabolic process
Disease: Pseudovaginal Perineoscrotal Hypospadias
|
|
TOP
|
|
| Research Articles on SRD5a2
|
|
1. This cohort study describes the phenotypic, biochemical and long-term outcome in 11 Chinese patients with 5alpha-RD2 deficiency and defines the genotypic spectrum of SRD5A2 mutations in China.
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with SRD5a2 elisa kit | Pathways associated with SRD5a2 elisa kit |
|
|
|
| Diseases associated with SRD5a2 elisa kit | Organs/Tissues associated with SRD5a2 elisa kit |
|
|
|
|
|
|
TOP
|
ELISA Kit 
