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SRD5a2 elisa kit :: Rat Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023257 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Standard Curve/Testing Data
Unit / Price
24-Strip-Wells  /  $305 +1 FREE 8GB USB
48-Strip-Wells  /  $575 +1 FREE 8GB USB
96-Strip-Wells  /  $780 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,175 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,730 +7 FREE 8GB USB
 
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 Product Name   

Steroid 5 Alpha Reductase 2 (SRD5a2), ELISA Kit

★Popular Item★
 Also Known As   

Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit

 Product Synonym Names    3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 2
 Product Gene Name   

SRD5a2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    264600
 3D Structure    ModBase 3D Structure for P31213
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Steroid 5 Alpha Reductase 2 (SRD5a2).
No significant cross-reactivity or interference between Steroid 5 Alpha Reductase 2 (SRD5a2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.065ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Steroid 5 Alpha Reductase 2 (SRD5a2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SRD5a2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SRD5a2 purchase    MBS2023257 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Steroid 5 Alpha Reductase 2 (SRD5a2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing SRD5a2, with an estimated sensitivity of < 0.065ng/mL. The ELISA analytical biochemical technique of the MBS2023257 kit is based on SRD5a2 antibody-SRD5a2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SRD5a2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SRD5a2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SRD5a2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Steroid 5 Alpha Reductase 2 (SRD5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Steroid 5 Alpha Reductase 2 (SRD5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Steroid 5 Alpha Reductase 2 (SRD5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for SRD5a2 elisa kit    Enzyme & Kinase
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 Typical Standard Curve/Testing Data of SRD5a2 elisa kit    SRD5a2 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2023257. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SRD5a2. It may not necessarily be applicable to this product.
 NCBI GI #    338469
 NCBI GeneID    6716
 NCBI Accession #    AAA60586.1 [Other Products]
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 UniProt Primary Accession #    P31213 [Other Products]
 UniProt Secondary Accession #    Q2M1R4; Q9BYE6; B2RE87 [Other Products]
 UniProt Related Accession #    P31213 [Other Products]
 Molecular Weight    28,393 Da
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 NCBI Official Full Name    steroid 5-alpha-reductase 2
 NCBI Official Synonym Full Names    steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)
 NCBI Official Symbol    SRD5A2 [Similar Products]
 NCBI Protein Information    3-oxo-5-alpha-steroid 4-dehydrogenase 2
 UniProt Protein Name    3-oxo-5-alpha-steroid 4-dehydrogenase 2
 UniProt Synonym Protein Names   
5 alpha-SR2; SR type 2; Steroid 5-alpha-reductase 2; S5AR 2; Type II 5-alpha reductase
 Protein Family    3-oxo-5-alpha-steroid 4-dehydrogenase
 UniProt Gene Name    SRD5A2 [Similar Products]
 UniProt Synonym Gene Names    S5AR 2 [Similar Products]
 UniProt Entry Name    S5A2_HUMAN
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 NCBI Summary for SRD5a2    This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]
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 UniProt Comments for SRD5a2    SRD5A2: Converts testosterone (T) into 5-alpha- dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH). A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. Belongs to the steroid 5-alpha reductase family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.3.1.22; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: endoplasmic reticulum membrane; cell soma; integral to membrane

Molecular Function: amide binding; cholestenone 5-alpha-reductase activity; sterol 5-alpha reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity

Biological Process: steroid metabolic process; response to drug; response to nutrient levels; response to peptide hormone stimulus; hypothalamus development; dibenzo-p-dioxin metabolic process; phthalate metabolic process; androgen metabolic process; male gonad development; hippocampus development; androgen biosynthetic process; response to testosterone stimulus; female genitalia development; cell-cell signaling; biphenyl metabolic process; male genitalia development; cell differentiation; response to follicle-stimulating hormone stimulus; steroid catabolic process

Disease: Pseudovaginal Perineoscrotal Hypospadias
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 Research Articles on SRD5a2    1. This cohort study describes the phenotypic, biochemical and long-term outcome in 11 Chinese patients with 5alpha-RD2 deficiency and defines the genotypic spectrum of SRD5A2 mutations in China.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with SRD5a2 elisa kitPathways associated with SRD5a2 elisa kit
 Reference Product  PubMed Publications
 CYP19A1 elisa kit  >155 publications with SRD5a2 and CYP19A1
 HSD17B2 elisa kit  >42 publications with SRD5a2 and HSD17B2
 AKR1C3 elisa kit  >22 publications with SRD5a2 and AKR1C3
 HSD3B2 elisa kit  >19 publications with SRD5a2 and HSD3B2
 HSD17B3 elisa kit  >16 publications with SRD5a2 and HSD17B3
 HSD3B1 elisa kit  >14 publications with SRD5a2 and HSD3B1
 AKR1D1 elisa kit  >5 publications with SRD5a2 and AKR1D1
 UGT2B15 elisa kit  >2 publications with SRD5a2 and UGT2B15
 HSD17B6 elisa kit  >1 publications with SRD5a2 and HSD17B6
 Products by Pathway  Pathway Diagram
 Androgen Biosynthesis Pathway antibodies  Androgen Biosynthesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Steroid Hormones And Vitamin D Pathway antibodies  Metabolism Of Steroid Hormones And Vitamin D Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
 Steroid Hormone Biosynthesis Pathway antibodies  Steroid Hormone Biosynthesis Pathway Diagram
 Allopregnanolone Biosynthesis Pathway antibodies  Allopregnanolone Biosynthesis Pathway Diagram
 Allopregnanolone Biosynthesis Pathway antibodies  Allopregnanolone Biosynthesis Pathway Diagram
Diseases associated with SRD5a2 elisa kitOrgans/Tissues associated with SRD5a2 elisa kit
 Disease Name  Pubmed Publications
 Genital Diseases, Male Antibodies  >458 publications with SRD5a2 and Genital Diseases, Male
 Urogenital Abnormalities Antibodies  >271 publications with SRD5a2 and Urogenital Abnormalities
 Prostatic Neoplasms Antibodies  >267 publications with SRD5a2 and Prostatic Neoplasms
 Disorders of Sex Development Antibodies  >247 publications with SRD5a2 and Disorders of Sex Development
 Hyperplasia Antibodies  >244 publications with SRD5a2 and Hyperplasia
 Prostatic Hyperplasia Antibodies  >215 publications with SRD5a2 and Prostatic Hyperplasia
 Alopecia Antibodies  >51 publications with SRD5a2 and Alopecia
 Breast Neoplasms Antibodies  >35 publications with SRD5a2 and Breast Neoplasms
 Growth Disorders Antibodies  >22 publications with SRD5a2 and Growth Disorders
 Liver Diseases Antibodies  >22 publications with SRD5a2 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >573 publications with SRD5a2 and Prostate
 Brain Antibodies  >239 publications with SRD5a2 and Brain
 Skin Antibodies  >207 publications with SRD5a2 and Skin
 Testis Antibodies  >168 publications with SRD5a2 and Testis
 Liver Antibodies  >162 publications with SRD5a2 and Liver
 Kidney Antibodies  >35 publications with SRD5a2 and Kidney
 Uterus Antibodies  >35 publications with SRD5a2 and Uterus
 Lung Antibodies  >12 publications with SRD5a2 and Lung
 Eye Antibodies  >11 publications with SRD5a2 and Eye
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