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SDF1 elisa kit :: Rat Stromal Cell Derived Factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722311 SDF1 elisa kit
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Stromal Cell Derived Factor 1 (SDF1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Stromal Cell Derived Factor 1 ELISA Kit

 Product Gene Name   

SDF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SDF-1. No significant cross-reactivity or interference between SDF-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDF-1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Assay Type    Competitive or Sandwich
 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SDF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SDF1 purchase    MBS722311 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stromal Cell Derived Factor 1 (SDF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDF1, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS722311 kit is based on SDF1 antibody-SDF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for SDF1 elisa kit

   Intended Uses: This SDF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||SDF-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF-1. Standards or samples are then added to the microtiter plate wells and SDF-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDF-1 are added to each well to "sandwich" the SDF-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SDF1 elisa kit    Immunology
NCBI/Uniprot data below describe general gene information for SDF1. It may not necessarily be applicable to this product.
 NCBI GI #    45383151
 NCBI GeneID    395180
 NCBI Accession #    NP_989841.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_204510.1 [Other Products]
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 NCBI Official Full Name    stromal cell-derived factor 1
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 12
 NCBI Official Symbol    CXCL12 [Similar Products]
 NCBI Official Synonym Symbols   
SDF1
[Similar Products]
 NCBI Protein Information    stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
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 Research Articles on SDF1    1. The unique and non-overlapping patterns of CXCL12 and CXCL14 expression in ocular tissues suggest that these two chemokines may interact and have important functions in cell proliferation, differentiation and migration during eye development.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SDF1 elisa kit
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
 Intestinal Immune Network For IgA Production Pathway antibodies  Intestinal Immune Network For IgA Production Pathway Diagram
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