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Sod1 elisa kit :: Rat Superoxide dismutase [Cu-Zn] ELISA Kit

Scan QR to view Datasheet Catalog #    MBS289512
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Superoxide dismutase [Cu-Zn] (Sod1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Superoxide dismutase [Cu-Zn] ELISA Kit

 Product Synonym Names    Superoxide dismutase [Cu-Zn]; Sod1; 1.15.1.1
 Product Gene Name   

Sod1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS289512 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P07632
 Species Reactivity    Rat
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 Assay Type    Competitive
 Detection Range    1.56-100 U/mL
 Sensitivity    0.78 U/mL
 Intra-Assay CV    <=5.6%
 Inter-Assay CV    <=9.6%
 Recovery    89%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Sod1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Sod1 purchase    MBS289512 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Superoxide dismutase [Cu-Zn] (Sod1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100 U/mL in biological research samples containing Sod1, with an estimated sensitivity of 0.78 U/mL. The ELISA analytical biochemical technique of the MBS289512 kit is based on Sod1 antibody-Sod1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Sod1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Sod1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Sod1 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Sod1 elisa kit    Sod1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS289512. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Sod1. It may not necessarily be applicable to this product.
 NCBI GI #    8394328
 NCBI GeneID    24786
 NCBI Accession #    NP_058746.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017050.1 [Other Products]
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 UniProt Primary Accession #    P07632 [Other Products]
 UniProt Related Accession #    P07632 [Other Products]
 Molecular Weight    15,912 Da
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 NCBI Official Full Name    superoxide dismutase
 NCBI Official Synonym Full Names    superoxide dismutase 1, soluble
 NCBI Official Symbol    Sod1 [Similar Products]
 NCBI Official Synonym Symbols   
CuZnSOD
[Similar Products]
 NCBI Protein Information    superoxide dismutase [Cu-Zn]
 UniProt Protein Name    Superoxide dismutase [Cu-Zn]
 Protein Family    Superoxide dismutase
 UniProt Gene Name    Sod1 [Similar Products]
 UniProt Entry Name    SODC_RAT
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 NCBI Summary for Sod1    catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen; involved in response to oxidative stress [RGD, Feb 2006]
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 UniProt Comments for Sod1    SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Oxidoreductase; EC 1.15.1.1; Nuclear receptor co-regulator; Apoptosis; Mitochondrial

Cellular Component: cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite cytoplasm; extracellular matrix; extracellular region; extracellular space; lysosome; mitochondrial intermembrane space; mitochondrion; myelin sheath; neuron projection; nucleoplasm; nucleus; peroxisome; plasma membrane; protein complex; secretory granule

Molecular Function: chaperone binding; copper ion binding; enzyme binding; identical protein binding; protein phosphatase 2B binding; Rac GTPase binding; superoxide dismutase activity; zinc ion binding

Biological Process: activation of MAPK activity; aging; anterograde axon cargo transport; auditory receptor cell stereocilium organization and biogenesis; cell aging; cellular iron ion homeostasis; DNA fragmentation during apoptosis; double-strand break repair; embryo implantation; glutathione metabolic process; heart contraction; hydrogen peroxide biosynthetic process; locomotory behavior; muscle maintenance; myelin maintenance in the peripheral nervous system; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of cholesterol biosynthetic process; negative regulation of neuron apoptosis; neurofilament cytoskeleton organization and biogenesis; ovarian follicle development; positive regulation of catalytic activity; positive regulation of cytokine production; positive regulation of superoxide release; regulation of blood pressure; regulation of GTPase activity; regulation of mitochondrial membrane potential; regulation of multicellular organism growth; regulation of protein kinase activity; relaxation of vascular smooth muscle; removal of superoxide radicals; response to amphetamine; response to antibiotic; response to axon injury; response to copper ion; response to drug; response to ethanol; response to heat; response to hydrogen peroxide; response to nutrient levels; response to organic substance; response to oxidative stress; response to reactive oxygen species; response to superoxide; retinal homeostasis; retrograde axon cargo transport; sensory perception of sound; spermatogenesis; superoxide metabolic process; superoxide release; transmission of nerve impulse
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 Research Articles on Sod1    1. The found of this study suggest that, from a histological standpoint, the SOD1-G93A rat is a valid model of ALS bulbar symptoms.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Sod1 elisa kitPathways associated with Sod1 elisa kit
 Reference Product  PubMed Publications
 Sod2 elisa kit  >470 publications with Sod1 and Sod2
 Gpx1 elisa kit  >159 publications with Sod1 and Gpx1
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 Prdx6 elisa kit  >10 publications with Sod1 and Prdx6
 Txn1 elisa kit  >6 publications with Sod1 and Txn1
 Prdx5 elisa kit  >4 publications with Sod1 and Prdx5
 Calm1 elisa kit  >2 publications with Sod1 and Calm1
 Tuba4a elisa kit  >1 publications with Sod1 and Tuba4a
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
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 Longevity Regulating Pathway - Multiple Species antibodies  Longevity Regulating Pathway - Multiple Species Diagram
 Longevity Regulating Pathway - Multiple Species antibodies  Longevity Regulating Pathway - Multiple Species Diagram
Diseases associated with Sod1 elisa kitOrgans/Tissues associated with Sod1 elisa kit
 Disease Name  Pubmed Publications
 Amyotrophic Lateral Sclerosis Antibodies  >2722 publications with Sod1 and Amyotrophic Lateral Sclerosis
 Nervous System Diseases Antibodies  >2645 publications with Sod1 and Nervous System Diseases
 Motor Neuron Disease Antibodies  >2414 publications with Sod1 and Motor Neuron Disease
 Disease Models, Animal Antibodies  >1163 publications with Sod1 and Disease Models, Animal
 Nerve Degeneration Antibodies  >331 publications with Sod1 and Nerve Degeneration
 Inflammation Antibodies  >210 publications with Sod1 and Inflammation
 Necrosis Antibodies  >132 publications with Sod1 and Necrosis
 Muscle Weakness Antibodies  >107 publications with Sod1 and Muscle Weakness
 Muscular Atrophy Antibodies  >102 publications with Sod1 and Muscular Atrophy
 Down Syndrome Antibodies  >81 publications with Sod1 and Down Syndrome
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1025 publications with Sod1 and Brain
 Muscle Antibodies  >580 publications with Sod1 and Muscle
 Liver Antibodies  >207 publications with Sod1 and Liver
 Heart Antibodies  >101 publications with Sod1 and Heart
 Kidney Antibodies  >98 publications with Sod1 and Kidney
 Artery Antibodies  >81 publications with Sod1 and Artery
 Lung Antibodies  >73 publications with Sod1 and Lung
 Eye Antibodies  >65 publications with Sod1 and Eye
 Ovary Antibodies  >30 publications with Sod1 and Ovary
 Spleen Antibodies  >22 publications with Sod1 and Spleen
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