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TLS elisa kit :: Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729871
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

T lymphocyte subpopulation CD3/4/8 (TLS), ELISA Kit

 Also Known As   

Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit

 Product Gene Name   

TLS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    614782
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 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of TLS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TLS purchase    MBS729871 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the T lymphocyte subpopulation CD3/4/8 (TLS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TLS, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS729871 kit is based on TLS antibody-TLS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TLS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TLS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TLS elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This TLS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TLS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY TLS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLS antibody and an TLS-HRP conjugate. The assay sample and buffer are incubated together with TLS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLS concentration since TLS from samples and TLS-HRP conjugate compete for the anti-TLS antibody binding site. Since the number of sites is limited, as more sites are occupied by TLS from the sample, fewer sites are left to bind TLS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLS concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TLS elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of TLS elisa kit    TLS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729871
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NCBI/Uniprot data below describe general gene information for TLS. It may not necessarily be applicable to this product.
 NCBI GI #    386157
 NCBI GeneID    2521
 NCBI Accession #    AAB27102.1 [Other Products]
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 UniProt Secondary Accession #    Q9H4A8 [Other Products]
 UniProt Related Accession #    P35637 [Other Products]
 Molecular Weight    53,355 Da
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 NCBI Official Full Name    TLS
 NCBI Official Synonym Full Names    FUS RNA binding protein
 NCBI Official Symbol    FUS [Similar Products]
 NCBI Official Synonym Symbols   
TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
[Similar Products]
 NCBI Protein Information    RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2
 UniProt Protein Name    RNA-binding protein FUS
 UniProt Synonym Protein Names   
75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein
 UniProt Gene Name    FUS [Similar Products]
 UniProt Synonym Gene Names    TLS [Similar Products]
 UniProt Entry Name    FUS_HUMAN
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 NCBI Summary for TLS    This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]
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 UniProt Comments for TLS    hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; RNA splicing; RNA-binding; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4
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 Research Articles on TLS    1. The FUS gene is not a genetic risk factor for Parkinson's disease in the population of Chinese Han ethnicity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TLS elisa kitPathways associated with TLS elisa kit
 Reference Product  PubMed Publications
 SRSF10 elisa kit  >3 publications with TLS and SRSF10
 HNRNPK elisa kit  >2 publications with TLS and HNRNPK
 YBX1 elisa kit  >2 publications with TLS and YBX1
 SRSF2 elisa kit  >2 publications with TLS and SRSF2
 SRRM1 elisa kit  >1 publications with TLS and SRRM1
 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
Diseases associated with TLS elisa kitOrgans/Tissues associated with TLS elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >443 publications with TLS and Nervous System Diseases
 Liposarcoma, Myxoid Antibodies  >113 publications with TLS and Liposarcoma, Myxoid
 Disease Models, Animal Antibodies  >58 publications with TLS and Disease Models, Animal
 Movement Disorders Antibodies  >38 publications with TLS and Movement Disorders
 Necrosis Antibodies  >26 publications with TLS and Necrosis
 Neoplasms, Experimental Antibodies  >21 publications with TLS and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >20 publications with TLS and Adenocarcinoma
 Breast Neoplasms Antibodies  >19 publications with TLS and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >17 publications with TLS and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >14 publications with TLS and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >301 publications with TLS and Brain
 Blood Antibodies  >192 publications with TLS and Blood
 Nerve Antibodies  >85 publications with TLS and Nerve
 Muscle Antibodies  >77 publications with TLS and Muscle
 Bone Antibodies  >63 publications with TLS and Bone
 Vascular Antibodies  >49 publications with TLS and Vascular
 Liver Antibodies  >47 publications with TLS and Liver
 Skin Antibodies  >35 publications with TLS and Skin
 Kidney Antibodies  >32 publications with TLS and Kidney
 Eye Antibodies  >28 publications with TLS and Eye
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