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Tie2 elisa kit :: Rat TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024243 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $265 +1 FREE 8GB USB
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $655 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TEK Tyrosine Kinase, Endothelial (Tie2), ELISA Kit

★Popular Item★
 Also Known As   

TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit

 Product Synonym Names    CD202b; CD202-B; TEK; VMCM1; Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2; Venous Malformations Multiple Cutaneous And Mucosal
 Product Gene Name   

Tie2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600195
 3D Structure    ModBase 3D Structure for Q02763
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TEK Tyrosine Kinase, Endothelial (Tie2).
No significant cross-reactivity or interference between TEK Tyrosine Kinase, Endothelial (Tie2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.135ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level TEK Tyrosine Kinase, Endothelial (Tie2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level TEK Tyrosine Kinase, Endothelial (Tie2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Tie2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Tie2 purchase    MBS2024243 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing Tie2, with an estimated sensitivity of < 0.135ng/mL. The ELISA analytical biochemical technique of the MBS2024243 kit is based on Tie2 antibody-Tie2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tie2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tie2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Tie2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TEK Tyrosine Kinase, Endothelial (Tie2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TEK Tyrosine Kinase, Endothelial (Tie2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for Tie2 elisa kit    Enzyme & Kinase; Tumor immunity; Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of Tie2 elisa kit    Tie2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024243. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Tie2. It may not necessarily be applicable to this product.
 NCBI GI #    112180440
 NCBI GeneID    7010
 NCBI Accession #    AAH35514.2 [Other Products]
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 UniProt Primary Accession #    Q02763 [Other Products]
 UniProt Secondary Accession #    Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2 [Other Products]
 UniProt Related Accession #    Q02763 [Other Products]
 Molecular Weight    109,141 Da
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 NCBI Official Full Name    TEK tyrosine kinase, endothelial
 NCBI Official Synonym Full Names    TEK tyrosine kinase, endothelial
 NCBI Official Symbol    TEK [Similar Products]
 NCBI Official Synonym Symbols   
TIE2; VMCM; TIE-2; VMCM1; CD202B
[Similar Products]
 NCBI Protein Information    angiopoietin-1 receptor
 UniProt Protein Name    Angiopoietin-1 receptor
 UniProt Synonym Protein Names   
Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD_antigen: CD202b
 Protein Family    Tyrosine-protein kinase receptor
 UniProt Gene Name    TEK [Similar Products]
 UniProt Synonym Gene Names    TIE2; VMCM; VMCM1; hTIE2 [Similar Products]
 UniProt Entry Name    TIE2_HUMAN
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 NCBI Summary for Tie2    This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
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 UniProt Comments for Tie2    TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: microvillus; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; basal plasma membrane; stress fiber; plasma membrane

Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; protein amino acid autophosphorylation; cell-matrix adhesion; heart development; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; organ regeneration; Tie receptor signaling pathway; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; regulation of vascular permeability; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal
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 Research Articles on Tie2    1. Tie-2 expression in colorectal cancer is much higher and valuable for assessing the prognosis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Tie2 elisa kitPathways associated with Tie2 elisa kit
 Reference Product  PubMed Publications
 ANGPT2 elisa kit  >47 publications with Tie2 and ANGPT2
 ANGPT1 elisa kit  >37 publications with Tie2 and ANGPT1
 GRB7 elisa kit  >3 publications with Tie2 and GRB7
 DOK2 elisa kit  >3 publications with Tie2 and DOK2
 PTPN11 elisa kit  >2 publications with Tie2 and PTPN11
 GRB2 elisa kit  >2 publications with Tie2 and GRB2
 SHC1 elisa kit  >1 publications with Tie2 and SHC1
 ANGPT4 elisa kit  >1 publications with Tie2 and ANGPT4
 Products by Pathway  Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram
Diseases associated with Tie2 elisa kitOrgans/Tissues associated with Tie2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >61 publications with Tie2 and Cardiovascular Diseases
 Neovascularization, Pathologic Antibodies  >54 publications with Tie2 and Neovascularization, Pathologic
 Inflammation Antibodies  >48 publications with Tie2 and Inflammation
 Neoplasms, Experimental Antibodies  >22 publications with Tie2 and Neoplasms, Experimental
 Heart Diseases Antibodies  >20 publications with Tie2 and Heart Diseases
 Necrosis Antibodies  >16 publications with Tie2 and Necrosis
 Adenocarcinoma Antibodies  >15 publications with Tie2 and Adenocarcinoma
 Lung Neoplasms Antibodies  >15 publications with Tie2 and Lung Neoplasms
 Liver Diseases Antibodies  >14 publications with Tie2 and Liver Diseases
 Hypertension Antibodies  >14 publications with Tie2 and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >218 publications with Tie2 and Vascular
 Muscle Antibodies  >62 publications with Tie2 and Muscle
 Embryonic Tissue Antibodies  >58 publications with Tie2 and Embryonic Tissue
 Lung Antibodies  >55 publications with Tie2 and Lung
 Brain Antibodies  >35 publications with Tie2 and Brain
 Kidney Antibodies  >28 publications with Tie2 and Kidney
 Eye Antibodies  >13 publications with Tie2 and Eye
 Connective Tissue Antibodies  >10 publications with Tie2 and Connective Tissue
 Uterus Antibodies  >10 publications with Tie2 and Uterus
 Mouth Antibodies  >10 publications with Tie2 and Mouth
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