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TNF elisa kit :: Rat TNF alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824520
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
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 Product Name   

TNF alpha, ELISA Kit

★Popular Item★
 Also Known As   

Rat TNF alpha ELISA Kit

 Product Synonym Names    TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 Product Gene Name   

TNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P16599
 Species Reactivity    Rat
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 Specificity    The Rat TNF alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat TNF alpha proteins within the range of 15.6 pg/ml - 1000 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
 Assay Type    Sandwich
 Sensitivity    The minimum detectable dose of Rat TNF alpha is typically less than 1 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TNF purchase    MBS824520 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TNF alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNF, with an estimated sensitivity of The minimum detectable dose of Rat TNF alpha is typically less than 1 pg/ml. The ELISA analytical biochemical technique of the MBS824520 kit is based on TNF antibody-TNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TNF elisa kit

   Principle of the assay: Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.2

Background: Sandwich Enzyme-Linked Immunosorbent Assay for Quantitative Detection of Rat TNF alpha Concentrations in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
 Applications Tested/Suitable for TNF elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of TNF elisa kit    TNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824520. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TNF. It may not necessarily be applicable to this product.
 NCBI GI #    6981662
 NCBI GeneID    24835
 NCBI Accession #    NP_036807.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012675.3 [Other Products]
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 UniProt Primary Accession #    P16599 [Other Products]
 UniProt Secondary Accession #    Q6EE11; Q9JI26; Q9JI27 [Other Products]
 UniProt Related Accession #    P16599 [Other Products]
 Molecular Weight    25,806 Da
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 NCBI Official Full Name    tumor necrosis factor
 NCBI Official Synonym Full Names    tumor necrosis factor
 NCBI Official Symbol    Tnf [Similar Products]
 NCBI Official Synonym Symbols   
Tnfa; RATTNF; TNF-alpha
[Similar Products]
 NCBI Protein Information    tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
 UniProt Protein Name    Tumor necrosis factor
 UniProt Synonym Protein Names   
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
 Protein Family    Tumor necrosis factor
 UniProt Gene Name    Tnf [Similar Products]
 UniProt Synonym Gene Names    Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2 [Similar Products]
 UniProt Entry Name    TNFA_RAT
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 NCBI Summary for TNF    acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]
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 UniProt Comments for TNF    TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Apoptosis; Membrane protein, integral

Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; plasma membrane; integral to membrane; intracellular; lipid raft; external side of plasma membrane; phagocytic cup

Molecular Function: identical protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; cellular extravasation; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; embryonic gut development; negative regulation of protein complex disassembly; positive regulation of cytokine production; response to drug; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; glucose metabolic process; positive regulation of interleukin-6 production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; response to mechanical stimulus; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; positive regulation of protein complex assembly; negative regulation of viral genome replication; response to hypoxia; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; response to lipopolysaccharide; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; detection of mechanical stimulus involved in sensory perception of pain; cell activation; defense response to Gram-positive bacterium; organ morphogenesis; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; response to activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; response to radiation; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; MAPKKK cascade; calcium-mediated signaling; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; positive regulation of fever; activation of MAPKKK activity; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; negative regulation of myoblast differentiation; leukocyte tethering or rolling; positive regulation of cytokine secretion; positive regulation of inflammatory response
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 Research Articles on TNF    1. the present study provides in vitro evidence that activation of Wnt signaling upregulates the TNF-alpha expression and might cause the degeneration of nucleus pulposus cells
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TNF elisa kitDiseases associated with TNF elisa kit
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >89322 publications with TNF and Necrosis
 Inflammation Antibodies  >31935 publications with TNF and Inflammation
 Vascular Diseases Antibodies  >7386 publications with TNF and Vascular Diseases
 Lung Diseases Antibodies  >6623 publications with TNF and Lung Diseases
 Liver Diseases Antibodies  >5022 publications with TNF and Liver Diseases
 Insulin Resistance Antibodies  >2468 publications with TNF and Insulin Resistance
 Fibrosis Antibodies  >2442 publications with TNF and Fibrosis
 Atherosclerosis Antibodies  >2357 publications with TNF and Atherosclerosis
 Colitis Antibodies  >2276 publications with TNF and Colitis
 Pain Antibodies  >2204 publications with TNF and Pain
Organs/Tissues associated with TNF elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >50683 publications with TNF and Blood
 Prostate Antibodies  >798 publications with TNF and Prostate
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