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TPO-Ab elisa kit :: Rat TPO-Ab ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2510987
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TPO-Ab, ELISA Kit

★Popular Item★
 Also Known As   

Rat TPO-Ab(Thrombopoietin Antibody)ELISA Kit

 Product Gene Name   

TPO-Ab elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P14650
 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and recombinant Rat TPO-Ab. No significant cross-reactivity or interference between Rat TPO-Ab and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    6.25-400ng/mL
 Sensitivity    Min: 3.75ng/mL; Max: 400ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPO-Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPO-Ab purchase    MBS2510987 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TPO-Ab, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25-400ng/mL in biological research samples containing TPO-Ab, with an estimated sensitivity of Min: 3.75ng/mL; Max: 400ng/mL. The ELISA analytical biochemical technique of the MBS2510987 kit is based on TPO-Ab antibody-TPO-Ab antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPO-Ab antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPO-Ab. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPO-Ab elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TPO-Ab concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to TPO-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for TPO-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPO-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPO-Ab.You can calculate the concentration of TPO-Ab in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TPO-Ab elisa kit    TPO-Ab elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2510987. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPO-Ab. It may not necessarily be applicable to this product.
 NCBI GI #    402766537
 NCBI GeneID    54314
 NCBI Accession #    NP_062226.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_019353.2 [Other Products]
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 UniProt Primary Accession #    P14650 [Other Products]
 UniProt Related Accession #    P14650 [Other Products]
 Molecular Weight    101,460 Da
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 NCBI Official Full Name    thyroid peroxidase
 NCBI Official Synonym Full Names    thyroid peroxidase
 NCBI Official Symbol    Tpo [Similar Products]
 NCBI Protein Information    thyroid peroxidase
 UniProt Protein Name    Thyroid peroxidase
 UniProt Gene Name    Tpo [Similar Products]
 UniProt Synonym Gene Names    TPO [Similar Products]
 UniProt Entry Name    PERT_RAT
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 NCBI Summary for TPO-Ab    membrane-bound glycoprotein; iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4 [RGD, Feb 2006]
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 UniProt Comments for TPO-Ab    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.
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 Research Articles on TPO-Ab    1. Thyroid peroxidase gene expression is induced by lipopolysaccharide involving nuclear factor (NF)-kappaB p65 subunit phosphorylation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TPO-Ab elisa kitPathways associated with TPO-Ab elisa kit
 Reference Product  PubMed Publications
 Tyr elisa kit  >10 publications with TPO-Ab and Tyr
 Tat elisa kit  >4 publications with TPO-Ab and Tat
 Iyd elisa kit  >4 publications with TPO-Ab and Iyd
 Tshb elisa kit  >2 publications with TPO-Ab and Tshb
 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway antibodies  Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway Diagram
 Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway antibodies  Thyroid Hormone Biosynthesis, Tyrosine => Triiodothyronine/thyroxine Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
Organs/Tissues associated with TPO-Ab elisa kit
 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >69 publications with TPO-Ab and Mixed
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