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TPO elisa kit :: Rat Thrombopoietin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS020824 TPO elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thrombopoietin (TPO), ELISA Kit

★Popular Item★
 Also Known As   

Rat Thrombopoietin ELISA Kit

 Product Gene Name   

TPO elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 3; NC_000003.11 (184089773..184095932, complement). Location: 3q27
 OMIM    187950
 3D Structure    ModBase 3D Structure for P40225
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between Rat TPO and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml - 500 pg/ml.
 Sensitivity    2.0 pg/ml.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100].
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TPO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TPO purchase    MBS020824 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thrombopoietin (TPO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml - 500 pg/ml in biological research samples containing TPO, with an estimated sensitivity of 2.0 pg/ml. The ELISA analytical biochemical technique of the MBS020824 kit is based on TPO antibody-TPO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TPO elisa kit

   Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPO concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TPO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPO and TPO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS020824. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TPO. It may not necessarily be applicable to this product.
 NCBI GI #    914226
 NCBI GeneID    7066
 NCBI Accession #    AAB33390.1 [Other Products]
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 UniProt Primary Accession #    P40225 [Other Products]
 UniProt Secondary Accession #    Q13020; Q15790; Q15791; Q15792; A1L3Y0; B7ZLR8; B9EGA8 [Other Products]
 UniProt Related Accession #    P40225 [Other Products]
 Molecular Weight    37,823 Da
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 NCBI Official Full Name    thrombopoietin
 NCBI Official Synonym Full Names    thrombopoietin
 NCBI Official Symbol    THPO [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    thrombopoietin; MPL ligand; c-mpl ligand; thrombopoietin nirs variant 1; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand
 UniProt Protein Name    Thrombopoietin
 UniProt Synonym Protein Names   
C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
 Protein Family    Thrombopoietin
 UniProt Gene Name    THPO [Similar Products]
 UniProt Synonym Gene Names    MGDF; ML; MGDF [Similar Products]
 UniProt Entry Name    TPO_HUMAN
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 NCBI Summary for TPO    Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternate splicing results in multiple transcript variants of this gene.[provided by RefSeq, Jun 2012]
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 UniProt Comments for TPO    THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: growth factor activity; hormone activity; cytokine activity

Biological Process: positive regulation of protein kinase B signaling cascade; platelet activation; cell proliferation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1
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 Research Articles on TPO    1. Increased TPO levels may increase both platelet count and platelet size, resulting in more hemostatic tendency, which may contribute to the progression of ischemic stroke.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TPO elisa kitPathways associated with TPO elisa kit
 Reference Product  PubMed Publications
 MPL elisa kit  >21 publications with TPO and MPL
 KITLG elisa kit  >6 publications with TPO and KITLG
 SELP elisa kit  >1 publications with TPO and SELP
 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Aggregation (Plug Formation) Pathway antibodies  Platelet Aggregation (Plug Formation) Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
Diseases associated with TPO elisa kit
 Disease Name  Pubmed Publications
 Hematologic Diseases Antibodies  >15 publications with TPO and Hematologic Diseases
 Neoplasms Antibodies  >9 publications with TPO and Neoplasms
 Thrombocytosis Antibodies  >8 publications with TPO and Thrombocytosis
 Drug Toxicity Antibodies  >4 publications with TPO and Drug Toxicity
 Anemia Antibodies  >4 publications with TPO and Anemia
 Lung Neoplasms Antibodies  >2 publications with TPO and Lung Neoplasms
 Carcinoma Antibodies  >2 publications with TPO and Carcinoma
 Thrombocythemia, Essential Antibodies  >2 publications with TPO and Thrombocythemia, Essential
 Heart Failure Antibodies  >1 publications with TPO and Heart Failure
 Hypertension Antibodies  >1 publications with TPO and Hypertension
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