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TG elisa kit :: Rat Thyroglobulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729685
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thyroglobulin (TG), ELISA Kit

★Popular Item★
 Also Known As   

Rat Thyroglobulin ELISA Kit

 Product Gene Name   

TG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608175
 3D Structure    ModBase 3D Structure for P01266
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 Species Reactivity    Rat
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    25-500 pg/mL
 Sensitivity    1.0pg/ml
 Intended Uses    This TG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TG purchase    MBS729685 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyroglobulin (TG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 25-500 pg/mL in biological research samples containing TG, with an estimated sensitivity of 1.0pg/ml. The ELISA analytical biochemical technique of the MBS729685 kit is based on TG antibody-TG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TG elisa kit

   Principle of the Assay: TG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TG. Standards or samples are then added to the microtiter plate wells and TG if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TG present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TG are added to each well to "sandwich" the TG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve
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 Typical Testing Data/Standard Curve (for reference only) of TG elisa kit    TG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS729685. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TG. It may not necessarily be applicable to this product.
 NCBI GI #    37174
 NCBI GeneID    7038
 NCBI Accession #    CAA29104.1 [Other Products]
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 UniProt Primary Accession #    P01266 [Other Products]
 UniProt Secondary Accession #    O15274; O43899; Q15593; Q15948; Q9NYR1; Q9NYR2; Q9UMZ0; Q9UNY3 [Other Products]
 UniProt Related Accession #    P01266 [Other Products]
 Molecular Weight    298,383 Da
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 NCBI Official Full Name    thyroglobulin
 NCBI Official Synonym Full Names    thyroglobulin
 NCBI Official Symbol    TG [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    thyroglobulin
 UniProt Protein Name    Thyroglobulin
 Protein Family    Thyroglobulin
 UniProt Gene Name    TG [Similar Products]
 UniProt Synonym Gene Names    Tg [Similar Products]
 UniProt Entry Name    THYG_HUMAN
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 NCBI Summary for TG    Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009]
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 UniProt Comments for TG    TG: Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Defects in TG are the cause of thyroid dyshormonogenesis 3 (TDH3). A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases. Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3). AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity

Biological Process: regulation of myelination; iodide transport; thyroid gland development; hormone biosynthetic process; signal transduction; thyroid hormone metabolic process

Disease: Thyroid Dyshormonogenesis 3; Autoimmune Thyroid Disease, Susceptibility To, 3
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 Research Articles on TG    1. Applying the optimized cutoff value of washout thyroglobulin of 1.8 ng/mL in patients with suspicious ultrasonographic features facilitates the diagnostic evaluation of neck lymph nodes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with TG elisa kitDiseases associated with TG elisa kit
 Products by Pathway  Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroid Hormone Synthesis Pathway antibodies  Thyroid Hormone Synthesis Pathway Diagram
 Thyroxine (Thyroid Hormone) Production Pathway antibodies  Thyroxine (Thyroid Hormone) Production Pathway Diagram
 Disease Name  Pubmed Publications
 Thyroid Diseases Antibodies  >2341 publications with TG and Thyroid Diseases
 Fatty Liver Antibodies  >1601 publications with TG and Fatty Liver
 Liver Diseases Antibodies  >1374 publications with TG and Liver Diseases
 Inflammation Antibodies  >1370 publications with TG and Inflammation
 Hypertension Antibodies  >1295 publications with TG and Hypertension
 Thyroid Neoplasms Antibodies  >1174 publications with TG and Thyroid Neoplasms
 Necrosis Antibodies  >821 publications with TG and Necrosis
 Mental Disorders Antibodies  >811 publications with TG and Mental Disorders
 Kidney Diseases Antibodies  >774 publications with TG and Kidney Diseases
 Goiter Antibodies  >635 publications with TG and Goiter
Organs/Tissues associated with TG elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15752 publications with TG and Blood
 Liver Antibodies  >3775 publications with TG and Liver
 Thyroid Antibodies  >3034 publications with TG and Thyroid
 Muscle Antibodies  >2236 publications with TG and Muscle
 Brain Antibodies  >2132 publications with TG and Brain
 Kidney Antibodies  >1209 publications with TG and Kidney
 Connective Tissue Antibodies  >1020 publications with TG and Connective Tissue
 Lymph Node Antibodies  >660 publications with TG and Lymph Node
 Intestine Antibodies  >580 publications with TG and Intestine
 Pancreas Antibodies  >426 publications with TG and Pancreas
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