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TRH elisa kit :: Rat Thyrotropin Releasing Hormone (TRH) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2700349 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,960 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,625 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thyrotropin Releasing Hormone (TRH), ELISA Kit

★Popular Item★
 Also Known As   

Rat Thyrotropin Releasing Hormone (TRH) ELISA Kit

 Product Synonym Names    TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
 Product Gene Name   

TRH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    M63582,  3D Structure    ModBase 3D Structure for P20396
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Thyrotropin Releasing Hormone (TRH).
No significant cross-reactivity or interference between Thyrotropin Releasing Hormone (TRH) and analogues was observed.
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 Assay Type    Competitive Inhibition
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    6.17-500pg/mL
 Sensitivity    < 2.43pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TRH purchase    MBS2700349 is a ready-to-use microwell, strip plate Competitive Inhibition ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thyrotropin Releasing Hormone (TRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.17-500pg/mL in biological research samples containing TRH, with an estimated sensitivity of < 2.43pg/mL. The ELISA analytical biochemical technique of the MBS2700349 kit is based on TRH antibody-TRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TRH elisa kit

   This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thyrotropin Releasing Hormone (TRH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thyrotropin Releasing Hormone (TRH) and unlabeled Thyrotropin Releasing Hormone (TRH) (Standards or samples) with the pre-coated antibody specific to Thyrotropin Releasing Hormone (TRH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thyrotropin Releasing Hormone (TRH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thyrotropin Releasing Hormone (TRH) in the sample.
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 Typical Testing Data/Standard Curve (for reference only) of TRH elisa kit    TRH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2700349. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TRH. It may not necessarily be applicable to this product.
 NCBI GI #    6005920
 NCBI GeneID    7200
 NCBI Accession #    NP_009048.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_007117.4 [Other Products]
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 UniProt Primary Accession #    P20396 [Other Products]
 UniProt Secondary Accession #    Q2TB83; B2R8R1 [Other Products]
 UniProt Related Accession #    P20396 [Other Products]
 Molecular Weight    27,404 Da
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 NCBI Official Full Name    thyrotropin releasing hormone preproprotein
 NCBI Official Synonym Full Names    thyrotropin releasing hormone
 NCBI Official Symbol    TRH [Similar Products]
 NCBI Official Synonym Symbols   
TRF; Pro-TRH
[Similar Products]
 NCBI Protein Information    thyrotropin releasing hormone
 UniProt Protein Name    Pro-thyrotropin-releasing hormone
 UniProt Synonym Protein Names   
Prothyroliberin
 Protein Family    Thyrotropin-releasing hormone
 UniProt Gene Name    TRH [Similar Products]
 UniProt Synonym Gene Names    Pro-TRH; TRH; TRF [Similar Products]
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 NCBI Summary for TRH    This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]
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 UniProt Comments for TRH    Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes.
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 Research Articles on TRH    1. TRH rs13097335 may have a protective role toward the development of breast cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TRH elisa kitPathways associated with TRH elisa kit
 Reference Product  PubMed Publications
 PRL elisa kit  >1751 publications with TRH and PRL
 GHRH elisa kit  >246 publications with TRH and GHRH
 CCK elisa kit  >62 publications with TRH and CCK
 TRHR elisa kit  >47 publications with TRH and TRHR
 NTS elisa kit  >14 publications with TRH and NTS
 GRP elisa kit  >8 publications with TRH and GRP
 GCG elisa kit  >2 publications with TRH and GCG
 GNRH1 elisa kit  >1 publications with TRH and GNRH1
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with TRH elisa kitOrgans/Tissues associated with TRH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1987 publications with TRH and Nervous System Diseases
 Brain Diseases Antibodies  >1675 publications with TRH and Brain Diseases
 THYROTROPIN-RELEASING HORMONE DEFICIENCY Antibodies  >282 publications with TRH and THYROTROPIN-RELEASING HORMONE DEFICIENCY
 Disease Models, Animal Antibodies  >130 publications with TRH and Disease Models, Animal
 Drug Toxicity Antibodies  >108 publications with TRH and Drug Toxicity
 Bipolar Disorder Antibodies  >102 publications with TRH and Bipolar Disorder
 Seizures Antibodies  >94 publications with TRH and Seizures
 Hypothermia Antibodies  >91 publications with TRH and Hypothermia
 Hypertension Antibodies  >87 publications with TRH and Hypertension
 Pain Antibodies  >83 publications with TRH and Pain
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4618 publications with TRH and Brain
 Ovary Antibodies  >123 publications with TRH and Ovary
 Lung Antibodies  >111 publications with TRH and Lung
 Eye Antibodies  >109 publications with TRH and Eye
 Pineal Gland Antibodies  >48 publications with TRH and Pineal Gland
 Bone Marrow Antibodies  >12 publications with TRH and Bone Marrow
 Cervix Antibodies  >3 publications with TRH and Cervix
 Uncharacterized Tissue Antibodies  >1 publications with TRH and Uncharacterized Tissue
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