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TFPI elisa kit :: Rat Tissue factor pathway inhibitor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS730482
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $470 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $470 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $675 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,850 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,850 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,340 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,340 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tissue factor pathway inhibitor (TFPI), ELISA Kit

★Popular Item★
 Also Known As   

Rat Tissue factor pathway inhibitor ELISA Kit

 Product Gene Name   

TFPI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    152310
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 Species Reactivity    Rat
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 Assay Type    Competitive or Sandwich
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Sensitivity    0.1 ng/mL.
 Intended Uses    This TFPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TFPI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TFPI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TFPI purchase    MBS730482 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tissue factor pathway inhibitor (TFPI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFPI, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS730482 kit is based on TFPI antibody-TFPI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFPI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFPI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TFPI elisa kit

   Principle of the Assay: TFPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFPI antibody and an TFPI-HRP conjugate. The assay sample and buffer are incubated together with TFPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFPI concentration since TFPI from samples and TFPI-HRP conjugate compete for the anti-TFPI antibody binding site. Since the number of sites is limited, as more sites are occupied by TFPI from the sample, fewer sites are left to bind TFPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFPI concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TFPI elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of TFPI elisa kit    TFPI elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS730482. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TFPI. It may not necessarily be applicable to this product.
 NCBI GI #    73760409
 NCBI GeneID    7035
 NCBI Accession #    NP_001027452.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001032281.2 [Other Products]
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 UniProt Secondary Accession #    O95103; Q53TS4 [Other Products]
 UniProt Related Accession #    P10646 [Other Products]
 Molecular Weight    28,653 Da
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 NCBI Official Full Name    tissue factor pathway inhibitor isoform b
 NCBI Official Synonym Full Names    tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
 NCBI Official Symbol    TFPI [Similar Products]
 NCBI Official Synonym Symbols   
EPI; TFI; LACI; TFPI1
[Similar Products]
 NCBI Protein Information    tissue factor pathway inhibitor; anti-convertin; extrinsic pathway inhibitor
 UniProt Protein Name    Tissue factor pathway inhibitor
 UniProt Synonym Protein Names   
Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI
 Protein Family    Tissue factor pathway inhibitor
 UniProt Gene Name    TFPI [Similar Products]
 UniProt Synonym Gene Names    LACI; TFPI1; TFPI; EPI; LACI [Similar Products]
 UniProt Entry Name    TFPI1_HUMAN
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 NCBI Summary for TFPI    This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TFPI    TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: extracellular space; cell surface; endoplasmic reticulum; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: blood coagulation, extrinsic pathway; blood coagulation
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 Research Articles on TFPI    1. Data indicate that the protein S sex hormone-binding globulin (SHBG)-like domain was important for binding and enhancement of tissue factor pathway inhibitor (TFPI).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TFPI elisa kitPathways associated with TFPI elisa kit
 Reference Product  PubMed Publications
 SERPINC1 elisa kit  >156 publications with TFPI and SERPINC1
 FGF2 elisa kit  >11 publications with TFPI and FGF2
 F10 elisa kit  >3 publications with TFPI and F10
 SDC4 elisa kit  >1 publications with TFPI and SDC4
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Syndecan-4-mediated Signaling Events Pathway antibodies  Syndecan-4-mediated Signaling Events Pathway Diagram
Diseases associated with TFPI elisa kitOrgans/Tissues associated with TFPI elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >526 publications with TFPI and Cardiovascular Diseases
 Inflammation Antibodies  >193 publications with TFPI and Inflammation
 Heart Diseases Antibodies  >132 publications with TFPI and Heart Diseases
 Venous Thrombosis Antibodies  >128 publications with TFPI and Venous Thrombosis
 Carcinoma Antibodies  >79 publications with TFPI and Carcinoma
 Necrosis Antibodies  >78 publications with TFPI and Necrosis
 Disease Models, Animal Antibodies  >76 publications with TFPI and Disease Models, Animal
 Myocardial Infarction Antibodies  >64 publications with TFPI and Myocardial Infarction
 Kidney Diseases Antibodies  >58 publications with TFPI and Kidney Diseases
 Pain Antibodies  >37 publications with TFPI and Pain
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1239 publications with TFPI and Blood
 Vascular Antibodies  >529 publications with TFPI and Vascular
 Embryonic Tissue Antibodies  >101 publications with TFPI and Embryonic Tissue
 Muscle Antibodies  >95 publications with TFPI and Muscle
 Lung Antibodies  >88 publications with TFPI and Lung
 Heart Antibodies  >84 publications with TFPI and Heart
 Kidney Antibodies  >80 publications with TFPI and Kidney
 Liver Antibodies  >78 publications with TFPI and Liver
 Umbilical Cord Antibodies  >46 publications with TFPI and Umbilical Cord
 Placenta Antibodies  >46 publications with TFPI and Placenta
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