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TGFb1 elisa kit :: Rat Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2701296 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $220 +1 FREE 8GB USB
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $470 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,960 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,625 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transforming Growth Factor Beta 1 (TGFb1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

 Product Synonym Names    TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
 Product Gene Name   

TGFb1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    X05839
 3D Structure
   ModBase 3D Structure for P01137
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Culture Supernates and Other Biological Fluids
 Detection Range    15.6-1000pg/mL
 Sensitivity    < 6.0pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFb1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFb1 purchase    MBS2701296 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000pg/mL in biological research samples containing TGFb1, with an estimated sensitivity of < 6.0pg/mL. The ELISA analytical biochemical technique of the MBS2701296 kit is based on TGFb1 antibody-TGFb1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFb1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFb1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Culture Supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for TGFb1 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of TGFb1 elisa kit    TGFb1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2701296. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFb1. It may not necessarily be applicable to this product.
 NCBI GI #    33431110
 NCBI Accession #    AAQ18642.1 [Other Products]
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 UniProt Primary Accession #    P01137 [Other Products]
 UniProt Secondary Accession #    Q9UCG4; A8K792 [Other Products]
 Molecular Weight    44,341 Da
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 NCBI Official Full Name    transforming growth factor beta 1, partial
 UniProt Protein Name    Transforming growth factor beta-1
 Protein Family    Transforming growth factor
 UniProt Gene Name    TGFB1 [Similar Products]
 UniProt Synonym Gene Names    TGFB; TGF-beta-1; LAP [Similar Products]
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 UniProt Comments for TGFb1    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed:25893292). Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed:25893292).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFb1 elisa kitPathways associated with TGFb1 elisa kit
 Reference Product  PubMed Publications
 SMAD2 elisa kit  >1295 publications with TGFb1 and SMAD2
 SMAD7 elisa kit  >527 publications with TGFb1 and SMAD7
 TGFBR3 elisa kit  >95 publications with TGFb1 and TGFBR3
 DCN elisa kit  >83 publications with TGFb1 and DCN
 TIMP1 elisa kit  >74 publications with TGFb1 and TIMP1
 TGFBR2 elisa kit  >67 publications with TGFb1 and TGFBR2
 TGFBR1 elisa kit  >60 publications with TGFb1 and TGFBR1
 LTBP1 elisa kit  >48 publications with TGFb1 and LTBP1
 FN1 elisa kit  >22 publications with TGFb1 and FN1
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with TGFb1 elisa kitOrgans/Tissues associated with TGFb1 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >4925 publications with TGFb1 and Fibrosis
 Lung Diseases Antibodies  >1558 publications with TGFb1 and Lung Diseases
 Liver Cirrhosis Antibodies  >847 publications with TGFb1 and Liver Cirrhosis
 Pulmonary Fibrosis Antibodies  >775 publications with TGFb1 and Pulmonary Fibrosis
 Hypertension Antibodies  >720 publications with TGFb1 and Hypertension
 Proteinuria Antibodies  >496 publications with TGFb1 and Proteinuria
 Diabetic Nephropathies Antibodies  >469 publications with TGFb1 and Diabetic Nephropathies
 Hypersensitivity Antibodies  >451 publications with TGFb1 and Hypersensitivity
 Asthma Antibodies  >417 publications with TGFb1 and Asthma
 Neoplasm Metastasis Antibodies  >381 publications with TGFb1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5420 publications with TGFb1 and Blood
 Muscle Antibodies  >3286 publications with TGFb1 and Muscle
 Kidney Antibodies  >2792 publications with TGFb1 and Kidney
 Bone Antibodies  >2368 publications with TGFb1 and Bone
 Lung Antibodies  >2095 publications with TGFb1 and Lung
 Liver Antibodies  >1981 publications with TGFb1 and Liver
 Connective Tissue Antibodies  >1771 publications with TGFb1 and Connective Tissue
 Heart Antibodies  >1252 publications with TGFb1 and Heart
 Skin Antibodies  >1206 publications with TGFb1 and Skin
 Bone Marrow Antibodies  >805 publications with TGFb1 and Bone Marrow
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