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TGFbeta3 elisa kit :: Rat Transforming Growth Factor beta3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS040221 TGFbeta3 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,650 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,205 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Transforming Growth Factor beta3 (TGFbeta3), ELISA Kit

 Also Known As   

Rat Transforming Growth Factor beta3 ELISA Kit

 Product Gene Name   

TGFbeta3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 5; NC_006092.3 (37739364..37747665, complement). Location: chromosome: 5
 3D Structure    ModBase 3D Structure for P16047
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between Rat TGFbeta3 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    62.5 pg/ml - 2000 pg/ml.
 Sensitivity    10 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TGFbeta3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TGFbeta3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGFbeta3 and TGFbeta3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGFbeta3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TGFbeta3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TGFbeta3 purchase    MBS040221 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Transforming Growth Factor beta3 (TGFbeta3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5 pg/ml - 2000 pg/ml in biological research samples containing TGFbeta3, with an estimated sensitivity of 10 pg/ml. The ELISA analytical biochemical technique of the MBS040221 kit is based on TGFbeta3 antibody-TGFbeta3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFbeta3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFbeta3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TGFbeta3 elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TGFbeta3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TGFbeta3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGFbeta3 and TGFbeta3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGFbeta3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS040221. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TGFbeta3. It may not necessarily be applicable to this product.
 NCBI GI #    45382047
 NCBI GeneID    396438
 NCBI Accession #    NP_990785.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_205454.1 [Other Products]
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 UniProt Primary Accession #    P16047 [Other Products]
 UniProt Related Accession #    P16047 [Other Products]
 Molecular Weight    47,078 Da
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 NCBI Official Full Name    transforming growth factor beta-3 preproprotein
 NCBI Official Synonym Full Names    transforming growth factor, beta 3
 NCBI Official Symbol    TGFB3 [Similar Products]
 NCBI Official Synonym Symbols   
TGF-beta3
[Similar Products]
 NCBI Protein Information    transforming growth factor beta-3; TGF-beta-3; transforming growth factor-beta3
 UniProt Protein Name    Transforming growth factor beta-3
 UniProt Gene Name    TGFB3 [Similar Products]
 UniProt Synonym Gene Names    TGF-beta-3; LAP [Similar Products]
 UniProt Entry Name    TGFB3_CHICK
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 UniProt Comments for TGFbeta3    Function: Involved in embryogenesis and cell differentiation.

Subunit structure: Homodimer; disulfide-linked.

Subcellular location: Secreted.

Post-translational modification: The precursor is cleaved into mature TGF-beta-3 and LAP, which remains non-covalently linked to mature TGF-beta-3 rendering it inactive

By similarity.

Sequence similarities: Belongs to the TGF-beta family.
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 Research Articles on TGFbeta3    1. MBNL1 negatively regulates cell invasion in the endocardial cushions by restricting the magnitude and timing of endocardial-derived TGFbeta3 production.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TGFbeta3 elisa kitPathways associated with TGFbeta3 elisa kit
 Reference Product  PubMed Publications
 TGFB2 elisa kit  >537 publications with TGFbeta3 and TGFB2
 TGFBR1 elisa kit  >7 publications with TGFbeta3 and TGFBR1
 TGFBR2 elisa kit  >7 publications with TGFbeta3 and TGFBR2
 VEGFA elisa kit  >5 publications with TGFbeta3 and VEGFA
 CLU elisa kit  >2 publications with TGFbeta3 and CLU
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
Diseases associated with TGFbeta3 elisa kitOrgans/Tissues associated with TGFbeta3 elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >177 publications with TGFbeta3 and Fibrosis
 Cleft Palate Antibodies  >91 publications with TGFbeta3 and Cleft Palate
 Disease Models, Animal Antibodies  >88 publications with TGFbeta3 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >82 publications with TGFbeta3 and Cardiovascular Diseases
 Inflammation Antibodies  >63 publications with TGFbeta3 and Inflammation
 Necrosis Antibodies  >57 publications with TGFbeta3 and Necrosis
 Heart Diseases Antibodies  >50 publications with TGFbeta3 and Heart Diseases
 Breast Neoplasms Antibodies  >35 publications with TGFbeta3 and Breast Neoplasms
 Lung Diseases Antibodies  >33 publications with TGFbeta3 and Lung Diseases
 Adenocarcinoma Antibodies  >28 publications with TGFbeta3 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >178 publications with TGFbeta3 and Muscle
 Embryonic Tissue Antibodies  >130 publications with TGFbeta3 and Embryonic Tissue
 Heart Antibodies  >76 publications with TGFbeta3 and Heart
 Brain Antibodies  >63 publications with TGFbeta3 and Brain
 Head Antibodies  >41 publications with TGFbeta3 and Head
 Adipose Tissue Antibodies  >26 publications with TGFbeta3 and Adipose Tissue
 Limb Antibodies  >23 publications with TGFbeta3 and Limb
 Ovary Antibodies  >14 publications with TGFbeta3 and Ovary
 Spleen Antibodies  >13 publications with TGFbeta3 and Spleen
 Epiphyseal Growth Plate Antibodies  >11 publications with TGFbeta3 and Epiphyseal Growth Plate
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