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TH elisa kit :: Rat Tyrosine Hydroxylase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744156
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tyrosine Hydroxylase (TH), ELISA Kit

★Popular Item★
 Also Known As   

Rat Tyrosine Hydroxylase ELISA Kit

 Product Gene Name   

TH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 605407
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of TH. No significant cross-reactivity or interference between TH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TH and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of TH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for TH purchase    MBS744156 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosine Hydroxylase (TH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TH, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS744156 kit is based on TH antibody-TH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for TH elisa kit

   Intended Uses: This TH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||TH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TH antibody and an TH-HRP conjugate. The assay sample and buffer are incubated together with TH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TH concentration since TH from samples and TH-HRP conjugate compete for the anti-TH antibody binding site. Since the number of sites is limited, as more sites are occupied by TH from the sample, fewer sites are left to bind TH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TH concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for TH elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve of TH elisa kit    TH elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS744156. Click to request current manual
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NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.
 NCBI GI #    986909
 NCBI GeneID    7054
 NCBI Accession #    AAA77650.1 [Other Products]
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 UniProt Secondary Accession #    Q0PWM2; Q0PWM3; Q15585; Q15588; Q15589; Q2M3B4; B7ZL70; B7ZL73 [Other Products]
 UniProt Related Accession #    P07101 [Other Products]
 Molecular Weight    44,898 Da
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 NCBI Official Full Name    tyrosine hydroxylase, partial
 NCBI Official Synonym Full Names    tyrosine hydroxylase
 NCBI Official Symbol    TH [Similar Products]
 NCBI Official Synonym Symbols   
TYH; DYT14; DYT5b
[Similar Products]
 NCBI Protein Information    tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
 UniProt Protein Name    Tyrosine 3-monooxygenase
 UniProt Synonym Protein Names   
Tyrosine 3-hydroxylase; TH
 UniProt Gene Name    TH [Similar Products]
 UniProt Synonym Gene Names    TYH; TH [Similar Products]
 UniProt Entry Name    TY3H_HUMAN
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 NCBI Summary for TH    The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TH    TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: EC 1.14.16.2; Mitochondrial; Oxidoreductase; Vesicle; Endoplasmic reticulum; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; cytoplasm; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to pyrethroid; neurotransmitter biosynthetic process; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; response to salt stress; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; memory; synaptic vesicle amine transport; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive
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 Research Articles on TH    1. Achilles tendon tenocytes produce tyrosine hydroxylase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with TH elisa kitPathways associated with TH elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >536375 publications with TH and JUN
 GAL elisa kit  >3280 publications with TH and GAL
 FOS elisa kit  >2718 publications with TH and FOS
 NGF elisa kit  >1816 publications with TH and NGF
 TYR elisa kit  >1603 publications with TH and TYR
 DDC elisa kit  >677 publications with TH and DDC
 FOSB elisa kit  >110 publications with TH and FOSB
 MAPKAPK2 elisa kit  >77 publications with TH and MAPKAPK2
 YWHAZ elisa kit  >15 publications with TH and YWHAZ
 CAMK2G elisa kit  >5 publications with TH and CAMK2G
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
Diseases associated with TH elisa kitOrgans/Tissues associated with TH elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >897516 publications with TH and Nervous System Diseases
 Heart Diseases Antibodies  >492540 publications with TH and Heart Diseases
 Brain Diseases Antibodies  >430100 publications with TH and Brain Diseases
 Pain Antibodies  >331494 publications with TH and Pain
 Kidney Diseases Antibodies  >214173 publications with TH and Kidney Diseases
 Inflammation Antibodies  >180142 publications with TH and Inflammation
 Hypertension Antibodies  >177018 publications with TH and Hypertension
 Necrosis Antibodies  >115341 publications with TH and Necrosis
 Myocardial Infarction Antibodies  >106976 publications with TH and Myocardial Infarction
 Arrhythmias, Cardiac Antibodies  >98132 publications with TH and Arrhythmias, Cardiac
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >292349 publications with TH and Brain
 Eye Antibodies  >149788 publications with TH and Eye
 Nerve Antibodies  >130926 publications with TH and Nerve
 Mouth Antibodies  >105370 publications with TH and Mouth
 Pancreas Antibodies  >42464 publications with TH and Pancreas
 Adrenal Gland Antibodies  >19745 publications with TH and Adrenal Gland
 Embryonic Tissue Antibodies  >17168 publications with TH and Embryonic Tissue
 Placenta Antibodies  >9286 publications with TH and Placenta
 Uncharacterized Tissue Antibodies  >110 publications with TH and Uncharacterized Tissue
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