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FOS elisa kit :: Rat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020036 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,775 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,035 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), ELISA Kit

★Popular Item★
 Also Known As   

V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

 Product Synonym Names    Cfos; C-Fos; G0S7; Proto-oncogene c-Fos; G0/G1 Switch Regulatory Protein 7; Cellular oncogene fos
 Product Gene Name   

FOS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    164810
 3D Structure    ModBase 3D Structure for P01100
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS).
No significant cross-reactivity or interference between V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.060ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FOS purchase    MBS2020036 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing FOS, with an estimated sensitivity of < 0.060ng/mL. The ELISA analytical biochemical technique of the MBS2020036 kit is based on FOS antibody-FOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FOS elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for FOS elisa kit    Tumor immunity
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 Typical Testing Data/Standard Curve (for reference only) of FOS elisa kit    FOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020036. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FOS. It may not necessarily be applicable to this product.
 NCBI GI #    13325364
 NCBI GeneID    2353
 NCBI Accession #    AAH04490.1 [Other Products]
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 UniProt Primary Accession #    P01100 [Other Products]
 UniProt Secondary Accession #    P18849; A8K4E2; B4DQ65 [Other Products]
 UniProt Related Accession #    P01100 [Other Products]
 Molecular Weight    36,301 Da
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 NCBI Official Full Name    V-fos FBJ murine osteosarcoma viral oncogene homolog
 NCBI Official Synonym Full Names    FBJ murine osteosarcoma viral oncogene homolog
 NCBI Official Symbol    FOS [Similar Products]
 NCBI Official Synonym Symbols   
p55; AP-1; C-FOS
[Similar Products]
 NCBI Protein Information    proto-oncogene c-Fos
 UniProt Protein Name    Proto-oncogene c-Fos
 UniProt Synonym Protein Names   
Cellular oncogene fos; G0/G1 switch regulatory protein 7
 Protein Family    Proto-oncogene
 UniProt Gene Name    FOS [Similar Products]
 UniProt Synonym Gene Names    G0S7 [Similar Products]
 UniProt Entry Name    FOS_HUMAN
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 NCBI Summary for FOS    The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FOS    Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: Oncoprotein; Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; nucleus; cytosol

Molecular Function: protein binding; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; response to lipopolysaccharide; female pregnancy; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; conditioned taste aversion; transforming growth factor beta receptor signaling pathway; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; response to corticosterone stimulus; aging; response to drug; nervous system development; response to light stimulus; MyD88-independent toll-like receptor signaling pathway; sleep; cellular response to hormone stimulus; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; cellular response to extracellular stimulus; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus
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 Research Articles on FOS    1. By targeting the proto-oncogene Fos, miR-101 is involved in G1-to-S phase transition in cervical cancer cells in vitro.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FOS elisa kitPathways associated with FOS elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >8060 publications with FOS and JUN
 JUNB elisa kit  >708 publications with FOS and JUNB
 JUND elisa kit  >501 publications with FOS and JUND
 MAPK1 elisa kit  >449 publications with FOS and MAPK1
 SRF elisa kit  >270 publications with FOS and SRF
 PTGS2 elisa kit  >237 publications with FOS and PTGS2
 ELK1 elisa kit  >179 publications with FOS and ELK1
 CREB1 elisa kit  >54 publications with FOS and CREB1
 CREM elisa kit  >52 publications with FOS and CREM
 MAPK8 elisa kit  >44 publications with FOS and MAPK8
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
Diseases associated with FOS elisa kitOrgans/Tissues associated with FOS elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2801 publications with FOS and Nervous System Diseases
 Pain Antibodies  >1412 publications with FOS and Pain
 Inflammation Antibodies  >989 publications with FOS and Inflammation
 Necrosis Antibodies  >742 publications with FOS and Necrosis
 Epilepsy Antibodies  >518 publications with FOS and Epilepsy
 Seizures Antibodies  >505 publications with FOS and Seizures
 Hyperalgesia Antibodies  >387 publications with FOS and Hyperalgesia
 Hypertension Antibodies  >375 publications with FOS and Hypertension
 Liver Diseases Antibodies  >371 publications with FOS and Liver Diseases
 Breast Neoplasms Antibodies  >362 publications with FOS and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10957 publications with FOS and Brain
 Blood Antibodies  >3591 publications with FOS and Blood
 Nerve Antibodies  >2573 publications with FOS and Nerve
 Muscle Antibodies  >1465 publications with FOS and Muscle
 Spinal Cord Antibodies  >1444 publications with FOS and Spinal Cord
 Bone Antibodies  >1228 publications with FOS and Bone
 Vascular Antibodies  >1163 publications with FOS and Vascular
 Liver Antibodies  >985 publications with FOS and Liver
 Heart Antibodies  >937 publications with FOS and Heart
 Skin Antibodies  >676 publications with FOS and Skin
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