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VCAM-1/CD106 elisa kit :: Rat VCAM-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2502676
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VCAM-1 (VCAM-1/CD106), ELISA Kit

★Popular Item★
 Also Known As   

Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit

 Product Gene Name   

VCAM-1/CD106 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2502676 COA    COA PDF
 3D Structure    ModBase 3D Structure for P29534
 Species Reactivity    Rat
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 Specificity    This kit recognizes natural and some recombinant Rat VCAM-1. No significant crossreactivity or interference between Rat VCAM-1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    12.5-800pg/mL
 Sensitivity    Min: 7.5pg/mL; Max: 800pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VCAM-1/CD106 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VCAM-1/CD106 purchase    MBS2502676 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VCAM-1 (VCAM-1/CD106) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5-800pg/mL in biological research samples containing VCAM-1/CD106, with an estimated sensitivity of Min: 7.5pg/mL; Max: 800pg/mL. The ELISA analytical biochemical technique of the MBS2502676 kit is based on VCAM-1/CD106 antibody-VCAM-1/CD106 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAM-1/CD106 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAM-1/CD106. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VCAM-1/CD106 elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat VCAM-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat VCAM-1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat VCAM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat VCAM-1, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat VCAM-1. The concentration of Rat VCAM-1 in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of VCAM-1/CD106 elisa kit    VCAM-1/CD106 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2502676. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VCAM-1/CD106. It may not necessarily be applicable to this product.
 NCBI GI #    6981700
 NCBI GeneID    25361
 NCBI Accession #    NP_037021.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012889.1 [Other Products]
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 UniProt Primary Accession #    P29534 [Other Products]
 UniProt Secondary Accession #    Q5FVS3; Q63669 [Other Products]
 UniProt Related Accession #    P29534 [Other Products]
 Molecular Weight    81,246 Da
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 NCBI Official Full Name    vascular cell adhesion protein 1
 NCBI Official Synonym Full Names    vascular cell adhesion molecule 1
 NCBI Official Symbol    Vcam1 [Similar Products]
 NCBI Official Synonym Symbols   
VCAM1B
[Similar Products]
 NCBI Protein Information    vascular cell adhesion protein 1; V-CAM 1; VCAM-1
 UniProt Protein Name    Vascular cell adhesion protein 1
 UniProt Synonym Protein Names   
CD_antigen: CD106
 UniProt Gene Name    Vcam1 [Similar Products]
 UniProt Synonym Gene Names    Vcam-1; V-CAM 1; VCAM-1 [Similar Products]
 UniProt Entry Name    VCAM1_RAT
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 NCBI Summary for VCAM-1/CD106    binds integrin very late antigen (VLA4); plays a role in cell adhesion [RGD, Feb 2006]
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 UniProt Comments for VCAM-1/CD106    VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; endoplasmic reticulum; apical part of cell; early endosome; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: response to nicotine; chronic inflammatory response; viral reproduction; heart development; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; acute inflammatory response; response to nutrient; aging
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 Research Articles on VCAM-1/CD106    1. Upregulation of the adhesion molecules VCAM-1, ICAM-1, and E-selectin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with VCAM-1/CD106 elisa kitDiseases associated with VCAM-1/CD106 elisa kit
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies  Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >83 publications with VCAM-1/CD106 and Inflammation
 Cardiovascular Diseases Antibodies  >54 publications with VCAM-1/CD106 and Cardiovascular Diseases
 Necrosis Antibodies  >49 publications with VCAM-1/CD106 and Necrosis
 Atherosclerosis Antibodies  >40 publications with VCAM-1/CD106 and Atherosclerosis
 Kidney Diseases Antibodies  >13 publications with VCAM-1/CD106 and Kidney Diseases
 Hypertension Antibodies  >12 publications with VCAM-1/CD106 and Hypertension
 Heart Diseases Antibodies  >11 publications with VCAM-1/CD106 and Heart Diseases
 Myocardial Ischemia Antibodies  >9 publications with VCAM-1/CD106 and Myocardial Ischemia
 Anemia Antibodies  >8 publications with VCAM-1/CD106 and Anemia
 Myocardial Infarction Antibodies  >7 publications with VCAM-1/CD106 and Myocardial Infarction
Organs/Tissues associated with VCAM-1/CD106 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Artery Antibodies  >31 publications with VCAM-1/CD106 and Artery
 Kidney Antibodies  >24 publications with VCAM-1/CD106 and Kidney
 Embryonic Tissue Antibodies  >21 publications with VCAM-1/CD106 and Embryonic Tissue
 Brain Antibodies  >19 publications with VCAM-1/CD106 and Brain
 Lung Antibodies  >13 publications with VCAM-1/CD106 and Lung
 Spleen Antibodies  >6 publications with VCAM-1/CD106 and Spleen
 Adipose Tissue Antibodies  >6 publications with VCAM-1/CD106 and Adipose Tissue
 Eye Antibodies  >3 publications with VCAM-1/CD106 and Eye
 Pancreas Antibodies  >3 publications with VCAM-1/CD106 and Pancreas
 Prostate Antibodies  >2 publications with VCAM-1/CD106 and Prostate
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