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VEGFA elisa kit :: Rat VEGF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175844
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF (VEGFA), ELISA Kit

★Popular Item★
 Also Known As   

Rat VEGF PicoKine ELISA Kit

 Product Synonym Names    Vascular endothelial growth factor A; vascular endothelial growth factor A; MGC70609; MVCD1; Vascular endothelial growth factor; Vascular endothelial growth factor A; Vascular permeability factor; VEGF A; VEGFA; VPF
 Product Gene Name   

VEGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for B5DEK7
 Species Reactivity    Rat
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 Specificity    Natural and recombinant rat VEGF
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 Samples    Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
 Assay Type    Quantitative Sandwich
 Sensitivity    < 1pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VEGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VEGFA purchase    MBS175844 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VEGF (VEGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VEGFA, with an estimated sensitivity of < 1pg/ml. The ELISA analytical biochemical technique of the MBS175844 kit is based on VEGFA antibody-VEGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma(heparin, EDTA, citrate). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VEGFA elisa kit

   Principle of the Assay: The rat VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGF has been precoated onto 96-well plates. Standards (NSQ,A27 -R 190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat VEGF amount of sample captured in plate.

Background/Introduction: Vascular permeability factor/va~)cular endothelial growth factor (VPFNEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.vEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.vEGF may also playa pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.
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 Typical Testing Data/Standard Curve (for reference only) of VEGFA elisa kit    Rat VEGF PicoKine ELISA Kit standard curve
VEGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175844. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VEGFA. It may not necessarily be applicable to this product.
 NCBI GeneID    83785
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 UniProt Primary Accession #    B5DEK7 [Other Products]
 UniProt Related Accession #    P16612 [Other Products]
 Molecular Weight    40,241 Da
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 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    Vegfa [Similar Products]
 NCBI Official Synonym Symbols   
VPF; Vegf; VEGF-A; VEGF164
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular endothelial growth factor A
 Protein Family    VEGF coregulated chemokine
 UniProt Gene Name    Vegfa [Similar Products]
 UniProt Entry Name    B5DEK7_RAT
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 NCBI Summary for VEGFA    This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]
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 UniProt Comments for VEGFA    VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; extracellular region; basement membrane; secretory granule

Molecular Function: heparin binding; identical protein binding; protein homodimerization activity; growth factor activity; extracellular matrix binding; cytokine activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor binding; receptor agonist activity; vascular endothelial growth factor receptor 2 binding; protein heterodimerization activity; fibronectin binding; receptor binding; chemoattractant activity

Biological Process: heart morphogenesis; macrophage differentiation; positive regulation of cell adhesion; positive regulation of positive chemotaxis; positive regulation of receptor internalization; cell maturation; female pregnancy; response to vitamin A; basophil chemotaxis; positive regulation of MAP kinase activity; regulation of cell shape; positive chemotaxis; response to folic acid; positive regulation of mesenchymal cell proliferation; mesoderm development; negative regulation of neuron apoptosis; kidney development; positive regulation of neuroblast proliferation; nervous system development; T-helper 1 type immune response; monocyte differentiation; positive regulation of signal transduction; mRNA stabilization; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; regulation of endothelial cell differentiation; positive regulation of vascular permeability; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; eye photoreceptor cell development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; camera-type eye morphogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cardiac muscle fiber development; positive regulation of cell division; activation of protein kinase activity; positive regulation of axon extension involved in axon guidance; neuron development; endothelial cell migration; blood vessel morphogenesis; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cGMP metabolic process; response to progesterone stimulus; alveolus development; surfactant homeostasis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; wound healing; positive regulation of smooth muscle cell proliferation; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; response to estradiol stimulus; induction of positive chemotaxis; vasculature development; epithelial cell differentiation; ovarian follicle development; positive regulation of focal adhesion formation; lymphangiogenesis; positive regulation of cell proliferation; negative regulation of programmed cell death; angiogenesis; cell differentiation; negative regulation of bone resorption; aging; blood vessel development; cell migration; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; blood vessel remodeling; response to cold; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; lung development; positive regulation of cell migration; growth
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 Research Articles on VEGFA    1. VEGF secreted by transplanted MSCs is one of the critical paracrine factors that play seminal roles in attenuating hyperoxic lung injuries in neonatal rats
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with VEGFA elisa kitDiseases associated with VEGFA elisa kit
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1790 publications with VEGFA and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1273 publications with VEGFA and Retinal Diseases
 Macular Degeneration Antibodies  >856 publications with VEGFA and Macular Degeneration
 Neoplasm Metastasis Antibodies  >463 publications with VEGFA and Neoplasm Metastasis
 Choroidal Neovascularization Antibodies  >435 publications with VEGFA and Choroidal Neovascularization
 Inflammation Antibodies  >426 publications with VEGFA and Inflammation
 Diabetic Retinopathy Antibodies  >406 publications with VEGFA and Diabetic Retinopathy
 Lung Diseases Antibodies  >375 publications with VEGFA and Lung Diseases
 Macular Edema Antibodies  >299 publications with VEGFA and Macular Edema
 Heart Diseases Antibodies  >258 publications with VEGFA and Heart Diseases
Organs/Tissues associated with VEGFA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1246 publications with VEGFA and Eye
 Lung Antibodies  >492 publications with VEGFA and Lung
 Muscle Antibodies  >425 publications with VEGFA and Muscle
 Embryonic Tissue Antibodies  >387 publications with VEGFA and Embryonic Tissue
 Heart Antibodies  >348 publications with VEGFA and Heart
 Liver Antibodies  >326 publications with VEGFA and Liver
 Brain Antibodies  >280 publications with VEGFA and Brain
 Placenta Antibodies  >215 publications with VEGFA and Placenta
 Connective Tissue Antibodies  >161 publications with VEGFA and Connective Tissue
 Colon Antibodies  >157 publications with VEGFA and Colon
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