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VEGFA elisa kit :: Rat VEGF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824771
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF (VEGFA), ELISA Kit

 Also Known As   

Rat VEGF ELISA Kit

 Product Synonym Names    VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
 Product Gene Name   

VEGFA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P16612
 Species Reactivity    Rat
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 Samples    Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
 Assay Type    Sandwich
 Detection Range    15.625 pg/ml - 1000 pg/ml
 Sensitivity    7 pg/ml
 Detection Method    Colorimetric
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VEGFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VEGFA purchase    MBS824771 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VEGF (VEGFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625 pg/ml - 1000 pg/ml in biological research samples containing VEGFA, with an estimated sensitivity of 7 pg/ml. The ELISA analytical biochemical technique of the MBS824771 kit is based on VEGFA antibody-VEGFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VEGFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VEGFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VEGFA elisa kit

   Sandwich ELISA for quantitative detection of Rat VEGF concentrations.
 Applications Tested/Suitable for VEGFA elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of VEGFA elisa kit    VEGFA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824771. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VEGFA. It may not necessarily be applicable to this product.
 NCBI GI #    160358797
 NCBI GeneID    83785
 NCBI Accession #    NP_001103804.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001110334.2 [Other Products]
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 UniProt Primary Accession #    P16612 [Other Products]
 UniProt Secondary Accession #    Q541S6; Q91ZE1; Q9JKX7; Q9QXG6; Q9QXG7 [Other Products]
 UniProt Related Accession #    P16612 [Other Products]
 Molecular Weight    17,189 Da
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 NCBI Official Full Name    vascular endothelial growth factor A isoform 3
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    Vegfa [Similar Products]
 NCBI Official Synonym Symbols   
VPF; Vegf; VEGF-A; VEGF164
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A; vascular permeability factor
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 Protein Family    VEGF coregulated chemokine
 UniProt Gene Name    Vegfa [Similar Products]
 UniProt Synonym Gene Names    Vegf; VEGF-A; VPF [Similar Products]
 UniProt Entry Name    VEGFA_RAT
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 NCBI Summary for VEGFA    This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]
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 UniProt Comments for VEGFA    VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; cell surface; membrane; cytoplasm; extracellular region; basement membrane; secretory granule

Molecular Function: heparin binding; identical protein binding; protein homodimerization activity; growth factor activity; extracellular matrix binding; cytokine activity; platelet-derived growth factor receptor binding; vascular endothelial growth factor receptor 1 binding; receptor agonist activity; vascular endothelial growth factor receptor binding; vascular endothelial growth factor receptor 2 binding; protein heterodimerization activity; fibronectin binding; receptor binding; chemoattractant activity

Biological Process: heart morphogenesis; positive regulation of cell adhesion; macrophage differentiation; positive regulation of positive chemotaxis; positive regulation of receptor internalization; cell maturation; female pregnancy; basophil chemotaxis; response to vitamin A; regulation of cell shape; positive regulation of MAP kinase activity; positive chemotaxis; response to folic acid; positive regulation of mesenchymal cell proliferation; mesoderm development; negative regulation of neuron apoptosis; kidney development; positive regulation of neuroblast proliferation; nervous system development; T-helper 1 type immune response; positive regulation of signal transduction; monocyte differentiation; mRNA stabilization; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of protein amino acid autophosphorylation; positive regulation of vascular permeability; regulation of endothelial cell differentiation; patterning of blood vessels; regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; camera-type eye morphogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; cardiac muscle fiber development; positive regulation of cell division; positive regulation of axon extension involved in axon guidance; activation of protein kinase activity; blood vessel morphogenesis; neuron development; endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cGMP metabolic process; response to progesterone stimulus; alveolus development; surfactant homeostasis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; lactation; post-embryonic camera-type eye development; wound healing; positive regulation of smooth muscle cell proliferation; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; response to estradiol stimulus; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; vasculature development; epithelial cell differentiation; positive regulation of focal adhesion formation; ovarian follicle development; lymphangiogenesis; positive regulation of cell proliferation; negative regulation of programmed cell death; angiogenesis; negative regulation of bone resorption; cell differentiation; aging; blood vessel development; cell migration; in utero embryonic development; lumen formation; positive regulation of cell motility; positive regulation of peptidyl-serine phosphorylation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of protein complex assembly; response to hypoxia; artery morphogenesis; blood vessel remodeling; sprouting angiogenesis; response to cold; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; hyaluronan metabolic process; lung development; growth; positive regulation of cell migration
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 Research Articles on VEGFA    1. These results suggest that activation of the VEGF/VEGFR signaling pathway is important in the formation of venous hypertension-induced DAVFs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with VEGFA elisa kitDiseases associated with VEGFA elisa kit
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cellular Response To Hypoxia Pathway antibodies  Cellular Response To Hypoxia Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1810 publications with VEGFA and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1288 publications with VEGFA and Retinal Diseases
 Macular Degeneration Antibodies  >868 publications with VEGFA and Macular Degeneration
 Neoplasm Metastasis Antibodies  >466 publications with VEGFA and Neoplasm Metastasis
 Choroidal Neovascularization Antibodies  >443 publications with VEGFA and Choroidal Neovascularization
 Inflammation Antibodies  >429 publications with VEGFA and Inflammation
 Diabetic Retinopathy Antibodies  >411 publications with VEGFA and Diabetic Retinopathy
 Lung Diseases Antibodies  >378 publications with VEGFA and Lung Diseases
 Macular Edema Antibodies  >304 publications with VEGFA and Macular Edema
 Heart Diseases Antibodies  >259 publications with VEGFA and Heart Diseases
Organs/Tissues associated with VEGFA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1265 publications with VEGFA and Eye
 Lung Antibodies  >494 publications with VEGFA and Lung
 Muscle Antibodies  >430 publications with VEGFA and Muscle
 Embryonic Tissue Antibodies  >390 publications with VEGFA and Embryonic Tissue
 Heart Antibodies  >348 publications with VEGFA and Heart
 Liver Antibodies  >331 publications with VEGFA and Liver
 Brain Antibodies  >282 publications with VEGFA and Brain
 Placenta Antibodies  >216 publications with VEGFA and Placenta
 Connective Tissue Antibodies  >161 publications with VEGFA and Connective Tissue
 Colon Antibodies  >157 publications with VEGFA and Colon
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