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VCAN elisa kit :: Rat Versican ELISA Kit

Scan QR to view Datasheet Catalog #    MBS025485 VCAN elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Versican (VCAN), ELISA Kit

★Popular Item★
 Also Known As   

Rat Versican ELISA Kit

 Product Gene Name   

VCAN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    118661
 3D Structure    ModBase 3D Structure for P13611
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 Species Reactivity    Rat
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 Specificity    No significant cross-reactivity or interference between Rat VCAN and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    3.12 ng/ml - 100 ng/ml.
 Sensitivity    1.0 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VCAN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat VCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VCAN and VCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of VCAN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for VCAN purchase    MBS025485 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Versican (VCAN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 ng/ml - 100 ng/ml in biological research samples containing VCAN, with an estimated sensitivity of 1.0 ng/ml. The ELISA analytical biochemical technique of the MBS025485 kit is based on VCAN antibody-VCAN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCAN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCAN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for VCAN elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VCAN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat VCAN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VCAN and VCAN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS025485. Click to request current manual
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NCBI/Uniprot data below describe general gene information for VCAN. It may not necessarily be applicable to this product.
 NCBI GI #    21361116
 NCBI GeneID    1462
 NCBI Accession #    NP_004376.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004385.4 [Other Products]
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 UniProt Primary Accession #    P13611 [Other Products]
 UniProt Secondary Accession #    P20754; Q13010; Q13189; Q15123; Q9UCL9; Q9UNW5 [Other Products]
 UniProt Related Accession #    P13611 [Other Products]
 Molecular Weight    372,820 Da
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 NCBI Official Full Name    versican core protein isoform 1
 NCBI Official Synonym Full Names    versican
 NCBI Official Symbol    VCAN [Similar Products]
 NCBI Official Synonym Symbols   
WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
[Similar Products]
 NCBI Protein Information    versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2
 UniProt Protein Name    Versican core protein
 UniProt Synonym Protein Names   
Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan 2; Glial hyaluronate-binding protein; GHAP; Large fibroblast proteoglycan; PG-M
 Protein Family    Versican core protein
 UniProt Gene Name    VCAN [Similar Products]
 UniProt Synonym Gene Names    CSPG2; Chondroitin sulfate proteoglycan 2; GHAP [Similar Products]
 UniProt Entry Name    CSPG2_HUMAN
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 NCBI Summary for VCAN    This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
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 UniProt Comments for VCAN    CSPG2: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1). WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. Belongs to the aggrecan/versican proteoglycan family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; intracellular membrane-bound organelle; membrane; Golgi lumen; extracellular region

Molecular Function: protein binding; glycosaminoglycan binding; extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: extracellular matrix organization and biogenesis; central nervous system development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; heart development; multicellular organismal development; cell recognition; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; osteoblast differentiation; chondroitin sulfate metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; cell adhesion; skeletal development

Disease: Wagner Vitreoretinopathy
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 Research Articles on VCAN    1. CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VCAN elisa kitPathways associated with VCAN elisa kit
 Reference Product  PubMed Publications
 DCN elisa kit  >71 publications with VCAN and DCN
 BGN elisa kit  >45 publications with VCAN and BGN
 SDC4 elisa kit  >6 publications with VCAN and SDC4
 SDC1 elisa kit  >5 publications with VCAN and SDC1
 SDC2 elisa kit  >3 publications with VCAN and SDC2
 SDC3 elisa kit  >1 publications with VCAN and SDC3
 GPC1 elisa kit  >1 publications with VCAN and GPC1
 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 CS/DS Degradation Pathway antibodies  CS/DS Degradation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Chondroitin Sulfate Biosynthesis Pathway antibodies  Chondroitin Sulfate Biosynthesis Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Dermatan Sulfate Biosynthesis Pathway antibodies  Dermatan Sulfate Biosynthesis Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
Diseases associated with VCAN elisa kitOrgans/Tissues associated with VCAN elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >101 publications with VCAN and Neoplasms
 Nervous System Diseases Antibodies  >29 publications with VCAN and Nervous System Diseases
 Inflammation Antibodies  >19 publications with VCAN and Inflammation
 Fibrosis Antibodies  >13 publications with VCAN and Fibrosis
 Heart Diseases Antibodies  >13 publications with VCAN and Heart Diseases
 Atherosclerosis Antibodies  >13 publications with VCAN and Atherosclerosis
 Skin Neoplasms Antibodies  >11 publications with VCAN and Skin Neoplasms
 Lung Neoplasms Antibodies  >7 publications with VCAN and Lung Neoplasms
 Kidney Diseases Antibodies  >6 publications with VCAN and Kidney Diseases
 Ovarian Neoplasms Antibodies  >6 publications with VCAN and Ovarian Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >69 publications with VCAN and Vascular
 Muscle Antibodies  >61 publications with VCAN and Muscle
 Connective Tissue Antibodies  >52 publications with VCAN and Connective Tissue
 Blood Antibodies  >52 publications with VCAN and Blood
 Skin Antibodies  >46 publications with VCAN and Skin
 Nerve Antibodies  >41 publications with VCAN and Nerve
 Brain Antibodies  >36 publications with VCAN and Brain
 Bone Antibodies  >29 publications with VCAN and Bone
 Embryonic Tissue Antibodies  >28 publications with VCAN and Embryonic Tissue
 Lung Antibodies  >25 publications with VCAN and Lung
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