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XBP1 elisa kit :: Rat X-Box Binding Protein 1 (XBP1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022472 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $295 +1 FREE 8GB USB
48-Strip-Wells  /  $605 +1 FREE 8GB USB
96-Strip-Wells  /  $820 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,375 +4 FREE 8GB USB
10x96-Strip-Wells  /  $6,125 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

X-Box Binding Protein 1 (XBP1), ELISA Kit

 Also Known As   

X-Box Binding Protein 1 (XBP1) ELISA Kit

 Product Synonym Names    TREB5; XBP2; Tax-responsive element-binding protein 5
 Product Gene Name   

XBP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of X-Box Binding Protein 1 (XBP1).
No significant cross-reactivity or interference between X-Box Binding Protein 1 (XBP1) and analogues was observed.
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 Samples    Tissue homogenates, Cell lysates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.054ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Other Notes    Small volumes of XBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for XBP1 purchase    MBS2022472 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the X-Box Binding Protein 1 (XBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing XBP1, with an estimated sensitivity of Typically less than 0.054ng/mL. The ELISA analytical biochemical technique of the MBS2022472 kit is based on XBP1 antibody-XBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XBP1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for XBP1 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Box Binding Protein 1 (XBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of X-Box Binding Protein 1 (XBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of XBP1 elisa kit    XBP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022472
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NCBI/Uniprot data below describe general gene information for XBP1. It may not necessarily be applicable to this product.
 NCBI GI #    312222696
 NCBI GeneID    416918
 NCBI Accession #    NP_001006192.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001006192.2 [Other Products]
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 NCBI Official Full Name    X-box binding protein 1
 NCBI Official Synonym Full Names    X-box binding protein 1
 NCBI Official Symbol    XBP1 [Similar Products]
 NCBI Protein Information    X-box binding protein 1
 Protein Family    X-box-binding protein
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 Research Articles on XBP1    1. Light-dependent and circadian clock-regulated activation of sterol regulatory element-binding protein, X-box-binding protein 1, and heat shock factor pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with XBP1 elisa kit
 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
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