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ALDH2 elisa kit :: Rat aldehyde dehydrogenase 2 family (mitochondrial) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700110
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
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 Product Name   

aldehyde dehydrogenase 2 family (mitochondrial) (ALDH2), ELISA Kit

 Also Known As   

Rat aldehyde dehydrogenase, ALDH ELISA Kit

 Product Synonym Names    Rat aldehyde dehydrogenase; ALDH ELISA Kit; ALDH-E2; ALDHI; ALDM; MGC1806; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; mitochondrial aldehyde dehydrogenase 2; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial)
 Product Gene Name   

ALDH2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700110 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P11884
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat ALDH2. No significant cross-reactivity or interference between Rat ALDH2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    0.312 ng/ml-20 ng/ml
 Sensitivity    0.078 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALDH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALDH2 purchase    MBS700110 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the aldehyde dehydrogenase 2 family (mitochondrial) (ALDH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml-20 ng/ml in biological research samples containing ALDH2, with an estimated sensitivity of 0.078 ng/ml. The ELISA analytical biochemical technique of the MBS700110 kit is based on ALDH2 antibody-ALDH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALDH2 elisa kit

   Introduction: Aldehyde dehydrogenases are a group of enzymes that catalyse the oxidation (dehydrogenation) of aldehydes. Mitochondrial Aldehyde Dehydrogenase is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, which leave the liver and are metabolized by the body's muscle and heart. There are three different classes of these enzymes in mammals: class 1 (low Km, cytosolic), class 2 (low Km, mitochondrial), and class 3 (high Km, such as those expressed in tumors, stomach and cornea). In all three classes constitutive and inducible forms exist. ALDH1 and ALDH2 are the most important enzymes for aldehyde oxidation, and both are tetrameric enzymes composed of 54kDA subunits. These enzymes are found in many tissues of the body, but are at the highest concentration in the liver.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ALDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ALDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ALDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of ALDH2 elisa kit    ALDH2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700110. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALDH2. It may not necessarily be applicable to this product.
 NCBI GI #    14192933
 NCBI GeneID    29539
 NCBI Accession #    NP_115792.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_032416.1 [Other Products]
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 UniProt Primary Accession #    P11884 [Other Products]
 UniProt Secondary Accession #    Q6Q288; Q6Q289; Q6Q290; Q8K3V8; Q91ZD7 [Other Products]
 UniProt Related Accession #    P11884 [Other Products]
 Molecular Weight    56,488 Da
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 NCBI Official Full Name    aldehyde dehydrogenase, mitochondrial
 NCBI Official Synonym Full Names    aldehyde dehydrogenase 2 family (mitochondrial)
 NCBI Official Symbol    Aldh2 [Similar Products]
 NCBI Protein Information    aldehyde dehydrogenase, mitochondrial; ALDH1; ALDH-E2; ALDH class 2; mitochondrial aldehyde dehydrogenase; aldehyde dehydrogenase 2, mitochondrial
 UniProt Protein Name    Aldehyde dehydrogenase, mitochondrial
 UniProt Synonym Protein Names   
ALDH class 2; ALDH-E2; ALDH1
 UniProt Gene Name    Aldh2 [Similar Products]
 UniProt Entry Name    ALDH2_RAT
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 UniProt Comments for ALDH2    ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - tryptophan; Secondary Metabolites Metabolism - limonene and pinene degradation; EC; Mitochondrial; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - histidine; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Lipid Metabolism - glycerolipid; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: identical protein binding; protein binding; aldehyde dehydrogenase (NAD) activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Biological Process: response to nicotine; response to hyperoxia; ethanol catabolic process; response to lipopolysaccharide; response to testosterone stimulus; response to progesterone stimulus; liver development; response to estradiol stimulus; negative regulation of apoptosis; cellular response to hormone stimulus
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 Research Articles on ALDH2    1. Ethanol was used induce ALDH2 activity, which decreased myocardial injury in diabetic rats.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ALDH2 elisa kitDiseases associated with ALDH2 elisa kit
 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Ascorbate And Aldarate Metabolism Pathway antibodies  Ascorbate And Aldarate Metabolism Pathway Diagram
 Ascorbate And Aldarate Metabolism Pathway antibodies  Ascorbate And Aldarate Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Ethanol Oxidation Pathway antibodies  Ethanol Oxidation Pathway Diagram
 Fatty Acid Omega Oxidation Pathway antibodies  Fatty Acid Omega Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 GABA Biosynthesis, Eukaryotes, Putrescine => GABA Pathway antibodies  GABA Biosynthesis, Eukaryotes, Putrescine => GABA Pathway Diagram
 Disease Name  Pubmed Publications
 Alcoholism Antibodies  >335 publications with ALDH2 and Alcoholism
 Genetic Predisposition to Disease Antibodies  >190 publications with ALDH2 and Genetic Predisposition to Disease
 Flushing Antibodies  >112 publications with ALDH2 and Flushing
 Liver Diseases Antibodies  >111 publications with ALDH2 and Liver Diseases
 Esophageal Neoplasms Antibodies  >96 publications with ALDH2 and Esophageal Neoplasms
 Vascular Diseases Antibodies  >91 publications with ALDH2 and Vascular Diseases
 Heart Diseases Antibodies  >77 publications with ALDH2 and Heart Diseases
 Nervous System Diseases Antibodies  >51 publications with ALDH2 and Nervous System Diseases
 Liver Neoplasms Antibodies  >43 publications with ALDH2 and Liver Neoplasms
 Hypertension Antibodies  >38 publications with ALDH2 and Hypertension
Organs/Tissues associated with ALDH2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >340 publications with ALDH2 and Blood
 Head Antibodies  >36 publications with ALDH2 and Head
 Embryonic Tissue Antibodies  >27 publications with ALDH2 and Embryonic Tissue
 Testis Antibodies  >25 publications with ALDH2 and Testis
 Ovary Antibodies  >11 publications with ALDH2 and Ovary
 Whole Body Antibodies  >3 publications with ALDH2 and Whole Body
 Fat Body Antibodies  >2 publications with ALDH2 and Fat Body
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