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alpha-SMA elisa kit :: Rat alpha Smooth Muscle Actin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720046
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells-(Competitive)  /  $455 +1 FREE 8GB USB
48-Strip-Wells-(Sandwich)  /  $455 +1 FREE 8GB USB
96-Strip-Wells-(Competitive)  /  $660 +1 FREE 8GB USB
96-Strip-Wells-(Sandwich)  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells-(Competitive)  /  $2,925 +3 FREE 8GB USB
5x96-Strip-Wells-(Sandwich)  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells-(Competitive)  /  $5,490 +6 FREE 8GB USB
10x96-Strip-Wells-(Sandwich)  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha Smooth Muscle Actin (alpha-SMA), ELISA Kit

★Popular Item★
 Also Known As   

Rat alpha Smooth Muscle Actin ELISA Kit

 Product Synonym Names    Rat a Smooth Muscle Actin ELISA Kit
 Product Gene Name   

alpha-SMA elisa kit

[Similar Products]
 Product Synonym Gene Name    a-SMA [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Competitive ELISA Manual Insert    Competitive ELISA PDF Manual
  Sandwich ELISA Manual Insert    Sandwich ELISA PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    614042
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SMAalpha. No significant cross-reactivity or interference between SMAalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SMAalpha and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive or Sandwich
 Detection Range    50-1000ng/mL
 Sensitivity    1.0ng/ml
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of alpha-SMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for alpha-SMA purchase    MBS720046 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha Smooth Muscle Actin (alpha-SMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000ng/mL in biological research samples containing alpha-SMA, with an estimated sensitivity of 1.0ng/ml. The ELISA analytical biochemical technique of the MBS720046 kit is based on alpha-SMA antibody-alpha-SMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha-SMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha-SMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for alpha-SMA elisa kit

   Intended Uses: This SMAalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SMAalpha.

Principle of the Assay: SMAalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SMAalpha. Standards or samples are then added to the microtiter plate wells and SMAalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SMAalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SMAalpha are added to each well to "sandwich" the SMAalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SMAalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMAalpha concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for alpha-SMA elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of alpha-SMA elisa kit    alpha-SMA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for alpha-SMA. It may not necessarily be applicable to this product.
 NCBI GI #    213688375
 NCBI GeneID    59
 NCBI Accession #    NP_001135417.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001141945.1 [Other Products]
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 UniProt Secondary Accession #    P03996; P04108; Q6FI19; B2R8A4 [Other Products]
 UniProt Related Accession #    P62736 [Other Products]
 Molecular Weight    42,009 Da
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 NCBI Official Full Name    actin, aortic smooth muscle
 NCBI Official Synonym Full Names    actin, alpha 2, smooth muscle, aorta
 NCBI Official Symbol    ACTA2 [Similar Products]
 NCBI Official Synonym Symbols   
AAT6; ACTSA; MYMY5
[Similar Products]
 NCBI Protein Information    actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
 UniProt Protein Name    Actin, aortic smooth muscle
 UniProt Synonym Protein Names   
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
 UniProt Gene Name    ACTA2 [Similar Products]
 UniProt Synonym Gene Names    ACTSA; ACTVS; GIG46 [Similar Products]
 UniProt Entry Name    ACTA_HUMAN
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 NCBI Summary for alpha-SMA    The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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 UniProt Comments for alpha-SMA    ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton

Molecular Function: protein kinase binding; ATP binding

Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction

Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6
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 Research Articles on alpha-SMA    1. Suggest dynamic interplay between transcriptional activators Pur-alpha/Pur-beta and repressors in regulating SMalphaA gene output during myofibroblast differentiation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with alpha-SMA elisa kitPathways associated with alpha-SMA elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >143 publications with alpha-SMA and TGFB1
 MYH11 elisa kit  >26 publications with alpha-SMA and MYH11
 TAGLN elisa kit  >10 publications with alpha-SMA and TAGLN
 CFL1 elisa kit  >1 publications with alpha-SMA and CFL1
 UBC elisa kit  >1 publications with alpha-SMA and UBC
 ACTN2 elisa kit  >1 publications with alpha-SMA and ACTN2
 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
Diseases associated with alpha-SMA elisa kitOrgans/Tissues associated with alpha-SMA elisa kit
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >221 publications with alpha-SMA and Fibrosis
 Cardiovascular Diseases Antibodies  >95 publications with alpha-SMA and Cardiovascular Diseases
 Inflammation Antibodies  >67 publications with alpha-SMA and Inflammation
 Liver Diseases Antibodies  >65 publications with alpha-SMA and Liver Diseases
 Disease Models, Animal Antibodies  >59 publications with alpha-SMA and Disease Models, Animal
 Liver Cirrhosis Antibodies  >48 publications with alpha-SMA and Liver Cirrhosis
 Kidney Diseases Antibodies  >41 publications with alpha-SMA and Kidney Diseases
 Breast Neoplasms Antibodies  >34 publications with alpha-SMA and Breast Neoplasms
 Aortic Aneurysm, Thoracic Antibodies  >30 publications with alpha-SMA and Aortic Aneurysm, Thoracic
 Heart Diseases Antibodies  >27 publications with alpha-SMA and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >578 publications with alpha-SMA and Muscle
 Vascular Antibodies  >176 publications with alpha-SMA and Vascular
 Blood Antibodies  >128 publications with alpha-SMA and Blood
 Eye Antibodies  >103 publications with alpha-SMA and Eye
 Lung Antibodies  >101 publications with alpha-SMA and Lung
 Connective Tissue Antibodies  >91 publications with alpha-SMA and Connective Tissue
 Liver Antibodies  >85 publications with alpha-SMA and Liver
 Kidney Antibodies  >73 publications with alpha-SMA and Kidney
 Skin Antibodies  >65 publications with alpha-SMA and Skin
 Embryonic Tissue Antibodies  >47 publications with alpha-SMA and Embryonic Tissue
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