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ENO1 elisa kit :: Rat alpha-enolase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS935486
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-enolase (ENO1), ELISA Kit

★Popular Item★
 Also Known As   

Rat alpha-enolase ELISA Kit

 Product Synonym Names    Rat alpha-enolase ELISA Kit; ENO1L1; MBP-1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase 1; non-neural enolase; phosphopyruvate hydratase; tau-crystallin; alpha-enolase
 Product Gene Name   

ENO1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS935486 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P04764
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat alpha-enolase. No significant cross-reactivity or interference between rat alpha-enolase and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.625 ng/ml - 40 ng/ml.
 Sensitivity    The minimum detectable dose of rat alpha-enolase is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ENO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ENO1 purchase    MBS935486 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-enolase (ENO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml - 40 ng/ml in biological research samples containing ENO1, with an estimated sensitivity of The minimum detectable dose of rat alpha-enolase is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS935486 kit is based on ENO1 antibody-ENO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ENO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ENO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ENO1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha-enolase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha-enolase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha-enolase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha-enolase bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ENO1 elisa kit    ENO1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS935486. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.
 NCBI GI #    158186651
 NCBI GeneID    24333
 NCBI Accession #    NP_001103378.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001109908.1 [Other Products]
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 UniProt Primary Accession #    P04764 [Other Products]
 UniProt Secondary Accession #    Q66HI3; Q6AYV3; Q6P504 [Other Products]
 UniProt Related Accession #    P04764 [Other Products]
 Molecular Weight    47,128 Da
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 NCBI Official Full Name    alpha-enolase
 NCBI Official Synonym Full Names    enolase 1, (alpha)
 NCBI Official Symbol    Eno1 [Similar Products]
 NCBI Official Synonym Symbols   
Nne
[Similar Products]
 NCBI Protein Information    alpha-enolase; alpha enolase; non-neural enolase; enolase 1, alpha non-neuron; 2-phospho-D-glycerate hydro-lyase
 UniProt Protein Name    Alpha-enolase
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
 Protein Family    Alpha-enolase
 UniProt Gene Name    Eno1 [Similar Products]
 UniProt Synonym Gene Names    Eno-1; NNE [Similar Products]
 UniProt Entry Name    ENOA_RAT
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 UniProt Comments for ENO1    ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Transcription, coactivator/corepressor; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11

Cellular Component: extrinsic to plasma membrane; extracellular space; neuron projection; phosphopyruvate hydratase complex; membrane; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; RNA binding; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: glycolysis; in utero embryonic development; response to virus; positive regulation of binding; negative regulation of cell growth; negative regulation of transcription, DNA-dependent
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 Research Articles on ENO1    1. this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ENO1 elisa kitDiseases associated with ENO1 elisa kit
 Products by Pathway  Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >58 publications with ENO1 and Neoplasms
 Lung Diseases Antibodies  >16 publications with ENO1 and Lung Diseases
 Lung Neoplasms Antibodies  >15 publications with ENO1 and Lung Neoplasms
 Nervous System Diseases Antibodies  >14 publications with ENO1 and Nervous System Diseases
 Brain Diseases Antibodies  >13 publications with ENO1 and Brain Diseases
 Arthritis, Rheumatoid Antibodies  >10 publications with ENO1 and Arthritis, Rheumatoid
 Adenocarcinoma Antibodies  >10 publications with ENO1 and Adenocarcinoma
 Inflammation Antibodies  >9 publications with ENO1 and Inflammation
 Breast Neoplasms Antibodies  >9 publications with ENO1 and Breast Neoplasms
 Liver Diseases Antibodies  >7 publications with ENO1 and Liver Diseases
Organs/Tissues associated with ENO1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >23 publications with ENO1 and Brain
 Lung Antibodies  >23 publications with ENO1 and Lung
 Muscle Antibodies  >13 publications with ENO1 and Muscle
 Liver Antibodies  >13 publications with ENO1 and Liver
 Eye Antibodies  >11 publications with ENO1 and Eye
 Heart Antibodies  >6 publications with ENO1 and Heart
 Kidney Antibodies  >5 publications with ENO1 and Kidney
 Ovary Antibodies  >4 publications with ENO1 and Ovary
 Connective Tissue Antibodies  >3 publications with ENO1 and Connective Tissue
 Prostate Antibodies  >3 publications with ENO1 and Prostate
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