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AFP elisa kit :: Rat alpha-fetoprotein (AFP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267612
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $290 +1 FREE 8GB USB
96-Strip-Wells  /  $450 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha-fetoprotein (AFP), ELISA Kit

 Also Known As   

Rat alpha-fetoprotein (AFP) ELISA Kit

 Product Synonym Names    Rat a-fetoprotein (AFP) ELISA Kit
 Product Gene Name   

AFP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    104150
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant AFP concentration.
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Other Notes    Small volumes of AFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AFP purchase    MBS267612 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-fetoprotein (AFP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing AFP, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS267612 kit is based on AFP antibody-AFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AFP. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant AFP concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AFP elisa kit

   Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat AFP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve of AFP elisa kit    AFP elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS267612
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NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.
 NCBI GI #    4501989
 NCBI GeneID    174
 NCBI Accession #    NP_001125.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001134.2 [Other Products]
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 UniProt Secondary Accession #    B2RBU3 [Other Products]
 UniProt Related Accession #    P02771 [Other Products]
 Molecular Weight    68,678 Da
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 NCBI Official Full Name    alpha-fetoprotein
 NCBI Official Synonym Full Names    alpha-fetoprotein
 NCBI Official Symbol    AFP [Similar Products]
 NCBI Official Synonym Symbols   
AFPD; FETA; HPAFP
[Similar Products]
 NCBI Protein Information    alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
 UniProt Protein Name    Alpha-fetoprotein
 UniProt Synonym Protein Names   
Alpha-1-fetoprotein; Alpha-fetoglobulin
 Protein Family    Alpha-fetoprotein
 UniProt Gene Name    AFP [Similar Products]
 UniProt Synonym Gene Names    HPAFP [Similar Products]
 UniProt Entry Name    FETA_HUMAN
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 NCBI Summary for AFP    This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AFP    AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
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 Research Articles on AFP    1. In patients with locally advanced hepatocellular carcinoma, the use of a combination of two biomarkers, AFP and PIVKA-II, appears useful in predicting treatment outcomes through the subdivision of prognostic groups.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with AFP elisa kitPathways associated with AFP elisa kit
 Reference Product  PubMed Publications
 JUN elisa kit  >884 publications with AFP and JUN
 GPC3 elisa kit  >50 publications with AFP and GPC3
 IGFBP1 elisa kit  >16 publications with AFP and IGFBP1
 FOXA1 elisa kit  >10 publications with AFP and FOXA1
 TP53 elisa kit  >4 publications with AFP and TP53
 FOXA3 elisa kit  >3 publications with AFP and FOXA3
 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with AFP elisa kitOrgans/Tissues associated with AFP elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5022 publications with AFP and Neoplasms
 Carcinoma Antibodies  >3860 publications with AFP and Carcinoma
 Liver Diseases Antibodies  >3548 publications with AFP and Liver Diseases
 Liver Neoplasms Antibodies  >3468 publications with AFP and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3155 publications with AFP and Carcinoma, Hepatocellular
 Adenocarcinoma Antibodies  >3074 publications with AFP and Adenocarcinoma
 Nervous System Diseases Antibodies  >1180 publications with AFP and Nervous System Diseases
 Liver Cirrhosis Antibodies  >976 publications with AFP and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >485 publications with AFP and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >419 publications with AFP and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4307 publications with AFP and Liver
 Blood Antibodies  >3363 publications with AFP and Blood
 Vascular Antibodies  >492 publications with AFP and Vascular
 Lung Antibodies  >367 publications with AFP and Lung
 Brain Antibodies  >309 publications with AFP and Brain
 Embryonic Tissue Antibodies  >276 publications with AFP and Embryonic Tissue
 Muscle Antibodies  >190 publications with AFP and Muscle
 Kidney Antibodies  >172 publications with AFP and Kidney
 Placenta Antibodies  >159 publications with AFP and Placenta
 Uterus Antibodies  >97 publications with AFP and Uterus
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