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alpha2-AP elisa kit :: Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS262375
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
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 Product Name   

alpha2-Antiplasmin (alpha2-AP), ELISA Kit

★Popular Item★
 Also Known As   

Rat alpha2-Antiplasmin (alpha2-AP) ELISA Kit

 Product Synonym Names    Rat a2-Antiplasmin (a2-AP) ELISA Kit
 Product Gene Name   

alpha2-AP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    613168
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    Up to 0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of alpha2-AP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for alpha2-AP purchase    MBS262375 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha2-Antiplasmin (alpha2-AP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing alpha2-AP, with an estimated sensitivity of Up to 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS262375 kit is based on alpha2-AP antibody-alpha2-AP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha2-AP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha2-AP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for alpha2-AP elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat alpha2-AP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of alpha2-AP elisa kit    alpha2-AP elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS262375. Click to request current manual
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NCBI/Uniprot data below describe general gene information for alpha2-AP. It may not necessarily be applicable to this product.
 NCBI GI #    115583663
 NCBI GeneID    5345
 NCBI Accession #    NP_000925.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000934.3 [Other Products]
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 UniProt Secondary Accession #    Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7 [Other Products]
 UniProt Related Accession #    P08697 [Other Products]
 Molecular Weight    47,907 Da
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 NCBI Official Full Name    alpha-2-antiplasmin isoform a
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
 NCBI Official Symbol    SERPINF2 [Similar Products]
 NCBI Official Synonym Symbols   
AAP; API; PLI; A2AP; ALPHA-2-PI
[Similar Products]
 NCBI Protein Information    alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
 UniProt Protein Name    Alpha-2-antiplasmin
 UniProt Synonym Protein Names   
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
 UniProt Gene Name    SERPINF2 [Similar Products]
 UniProt Synonym Gene Names    AAP; PLI; Alpha-2-AP; Alpha-2-PI [Similar Products]
 UniProt Entry Name    A2AP_HUMAN
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 NCBI Summary for alpha2-AP    This gene encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
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 UniProt Comments for alpha2-AP    SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; protease binding; endopeptidase inhibitor activity

Biological Process: platelet activation; collagen fibril organization; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; response to organic substance; fibrinolysis; negative regulation of fibrinolysis; platelet degranulation; renin-angiotensin regulation of blood vessel size; positive regulation of stress fiber formation; acute-phase response; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; blood coagulation

Disease: Alpha-2-plasmin Inhibitor Deficiency
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 Research Articles on alpha2-AP    1. protective mediator during gram-negative (pneumo)sepsis, limiting bacterial growth, inflammation, tissue injury, and coagulation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with alpha2-AP elisa kitDiseases associated with alpha2-AP elisa kit
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3 publications with alpha2-AP and Liver Diseases
 Cardiovascular Diseases Antibodies  >2 publications with alpha2-AP and Cardiovascular Diseases
 Hepatitis Antibodies  >1 publications with alpha2-AP and Hepatitis
 Kidney Diseases Antibodies  >1 publications with alpha2-AP and Kidney Diseases
 Fibrosis Antibodies  >1 publications with alpha2-AP and Fibrosis
 Neoplasms Antibodies  >1 publications with alpha2-AP and Neoplasms
 Liver Neoplasms Antibodies  >1 publications with alpha2-AP and Liver Neoplasms
 Inflammation Antibodies  >1 publications with alpha2-AP and Inflammation
 Carcinoma, Hepatocellular Antibodies  >1 publications with alpha2-AP and Carcinoma, Hepatocellular
 Blood Coagulation Disorders Antibodies  >1 publications with alpha2-AP and Blood Coagulation Disorders
Organs/Tissues associated with alpha2-AP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with alpha2-AP and Blood
 Liver Antibodies  >4 publications with alpha2-AP and Liver
 Kidney Antibodies  >1 publications with alpha2-AP and Kidney
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