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AMY2A elisa kit :: Rat amylase, alpha 2A (pancreatic) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS945823
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

amylase, alpha 2A (pancreatic) (AMY2A), ELISA Kit

★Popular Item★
 Also Known As   

Rat Pancreatic alpha-amylase, AMY2A ELISA Kit

 Product Synonym Names    Rat Pancreatic alpha-amylase (AMY2A) ELISA kit; AMY2; AMY2B; PA; 1; 4-alpha-D-glucan glucanohydrolase; alpha-amylase; amylase; pancreatic; alpha-2A; found in the pancreas; glycogenase; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase; alpha 2A (pancreatic)
 Product Gene Name   

AMY2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS945823 COA    COA PDF
 MBS945823 Testing Data    Testing Data PDF
 OMIM    104650
 3D Structure    ModBase 3D Structure for P04746
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat AMY2A. No significant cross-reactivity or interference between rat AMY2A and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich (Quantitative)
 Detection Range    3.12 mIU/ml-200 mIU/ml.
 Sensitivity    0.78 mIU/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of AMY2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for AMY2A purchase    MBS945823 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amylase, alpha 2A (pancreatic) (AMY2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 mIU/ml-200 mIU/ml in biological research samples containing AMY2A, with an estimated sensitivity of 0.78 mIU/ml. The ELISA analytical biochemical technique of the MBS945823 kit is based on AMY2A antibody-AMY2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMY2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMY2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for AMY2A elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of AMY2A elisa kit    AMY2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS945823. Click to request current manual
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NCBI/Uniprot data below describe general gene information for AMY2A. It may not necessarily be applicable to this product.
 NCBI GI #    4502085
 NCBI GeneID    279
 NCBI Accession #    NP_000690.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000699.2 [Other Products]
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 UniProt Primary Accession #    P04746 [Other Products]
 UniProt Secondary Accession #    Q9UBH3; B9EJG1 [Other Products]
 UniProt Related Accession #    P04746 [Other Products]
 Molecular Weight    57,707 Da
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 NCBI Official Full Name    pancreatic alpha-amylase
 NCBI Official Synonym Full Names    amylase, alpha 2A (pancreatic)
 NCBI Official Symbol    AMY2A [Similar Products]
 NCBI Official Synonym Symbols   
PA; AMY2; AMY2B
[Similar Products]
 NCBI Protein Information    pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
 UniProt Protein Name    Pancreatic alpha-amylase
 UniProt Synonym Protein Names   
1,4-alpha-D-glucan glucanohydrolase
 UniProt Gene Name    AMY2A [Similar Products]
 UniProt Synonym Gene Names    PA [Similar Products]
 UniProt Entry Name    AMYP_HUMAN
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 NCBI Summary for AMY2A    Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
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 UniProt Comments for AMY2A    AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted; Hydrolase; Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis
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 Research Articles on AMY2A    1. Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with AMY2A elisa kitDiseases associated with AMY2A elisa kit
 Products by Pathway  Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Digestion Of Dietary Carbohydrate Pathway antibodies  Digestion Of Dietary Carbohydrate Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Starch And Sucrose Metabolism Pathway antibodies  Starch And Sucrose Metabolism Pathway Diagram
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >4 publications with AMY2A and Adenocarcinoma
 Neoplasms Antibodies  >4 publications with AMY2A and Neoplasms
 Pancreatic Neoplasms Antibodies  >3 publications with AMY2A and Pancreatic Neoplasms
 Lung Neoplasms Antibodies  >2 publications with AMY2A and Lung Neoplasms
 Lung Diseases Antibodies  >2 publications with AMY2A and Lung Diseases
 Inflammation Antibodies  >1 publications with AMY2A and Inflammation
Organs/Tissues associated with AMY2A elisa kit
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >4 publications with AMY2A and Pancreas
 Bladder Antibodies  >1 publications with AMY2A and Bladder
 Salivary Gland Antibodies  >1 publications with AMY2A and Salivary Gland
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