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Abeta1-42 elisa kit :: Rat amyloid beta peptide 1-42 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS726579
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

amyloid beta peptide 1-42 (Abeta1-42), ELISA Kit

 Also Known As   

Rat amyloid beta peptide 1-42 ELISA Kit

 Product Synonym Names    Rat amyloid beta peptide 1-42 ELISA Kit; amyloid beta peptide 1-42; amyloid beta peptide 1-42 (Rat)
 Product Gene Name   

Abeta1-42 elisa kit

[Similar Products]
 Product Synonym Gene Name    Ab1-42; Aβ1-42 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 16; NC_000082.6 (84954436..85173707, complement). Location: 16 C3-qter; 16 46.92 cM
 3D Structure    ModBase 3D Structure for P12023
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 Species Reactivity    Rat
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 Assay Type    Sandwich
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Abeta1-42 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Abeta1-42 purchase    MBS726579 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the amyloid beta peptide 1-42 (Abeta1-42) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Abeta1-42. The ELISA analytical biochemical technique of the MBS726579 kit is based on Abeta1-42 antibody-Abeta1-42 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Abeta1-42 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Abeta1-42. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Abeta1-42 elisa kit

   Intended Uses: This Abeta1-42 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Abeta1-42 in the sample, this Abeta1-42 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Abeta1-42 concentration. The concentration ofAbeta1-42 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This Abeta1-42 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Abeta1-42. Standards or samples are then added to the microtiter plate wells and Abeta1-42 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Abeta1-42 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Abeta1-42 are added to each well to "sandwich" the Abeta1-42 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain Abeta1-42 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for Abeta1-42 elisa kit    Rat ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of Abeta1-42 elisa kit    Abeta1-42 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS726579. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Abeta1-42. It may not necessarily be applicable to this product.
 NCBI GI #    311893408
 NCBI GeneID    11820
 NCBI Accession #    NP_001185755.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001198826.1 [Other Products]
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 UniProt Primary Accession #    P12023 [Other Products]
 UniProt Secondary Accession #    P97487; P97942; Q99K32 [Other Products]
 UniProt Related Accession #    P12023 [Other Products]
 Molecular Weight    86,722 Da
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 NCBI Official Full Name    amyloid beta A4 protein isoform 6
 NCBI Official Synonym Full Names    amyloid beta (A4) precursor protein
 NCBI Official Symbol    App [Similar Products]
 NCBI Official Synonym Symbols   
Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
[Similar Products]
 NCBI Protein Information    amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog
 UniProt Protein Name    Amyloid beta A4 protein
 UniProt Synonym Protein Names   
ABPP; APP; Alzheimer disease amyloid A4 protein homolog; Amyloidogenic glycoprotein; AGCleaved into the following 14 chains:N-APPSoluble APP-alpha; S-APP-alphaSoluble APP-beta; S-APP-betaC99; Alternative name(s):; APP-C99Beta-amyloid protein 42; Alternative name(s):; Beta-APP42Beta-amyloid protein 40; Alternative name(s):; Beta-APP40C83P3(42)P3(40)C80Gamma-secretase C-terminal fragment 59; Alternative name(s):; APP-C59; Amyloid intracellular domain 59; AID(59); Gamma-CTF(59)Gamma-secretase C-terminal fragment 57; Alternative name(s):; APP-C57; Amyloid intracellular domain 57; AID(57); Gamma-CTF(57)Gamma-secretase C-terminal fragment 50; Alternative name(s):; Amyloid intracellular domain 50; AID(50); Gamma-CTF(50)
 UniProt Gene Name    App [Similar Products]
 UniProt Synonym Gene Names    APP; AG; S-APP-alpha; S-APP-beta; AID(59); AID(57); AID(50) [Similar Products]
 UniProt Entry Name    A4_MOUSE
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 UniProt Comments for Abeta1-42    Function: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity

By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV

By similarity. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons

By similarity. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Ref.18Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Binds transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Also bind GPC1 in lipid rafts

By similarity. Ref.18The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. Ref.18N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6)

By similarity. Ref.18

Subunit structure: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, NUMB and DAB1. Binding to DAB1 inhibits its serine phosphorylation. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1

By similarity. In vitro, it binds MAPT via the MT-binding domains

By similarity. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner

By similarity. Interacts, through a C-terminal domain, with GNAO1

By similarity. Amyloid beta-42 binds CHRNA7 in hippocampal neurons

By similarity. Beta-amyloid associates with HADH2

By similarity. Interacts with ANKS1B, TNFRSF21 and AGER

By similarity. Interacts with CPEB1. Interacts with ITM2B. Interacts with ITM2C. Interacts with IDE. Can form homodimers; this is promoted by heparin binding

By similarity. Beta-amyloid protein 40 interacts with S100A9

By similarity. CTF-alpha product of APP interacts with GSAP

By similarity. Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19

Subcellular location: Membrane; Single-pass type I membrane protein. Membrane › clathrin-coated pit. Note: Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF59 peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FPRL1 at the cell surface and the complex is then rapidly internalized

By similarity. APP sorts to the basolateral surface in epithelial cells

By similarity. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. Associates with GPC1 in perinuclear compartments. Ref.18

Tissue specificity: Isoform APP770 is expressed in kidney. Isoform APP751 is widely expressed. Isoform APP695 is expressed in brain, kidney and liver. Isoform APP695, isoform APP714 and isoform APP751 are expressed in several different brain regions including hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, these isoforms are abundantly expressed in Purkinje cells. Ref.10

Domain: The basolateral sorting signal (BaSS) is required for sorting of membrane proteins to the basolateral surface of epithelial cells.The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The PID domain-containing proteins which bind APP require the YENPTY motif for full interaction. These interactions are independent of phosphorylation on the terminal tyrosine residue. The NPXY site is also involved in clathrin-mediated endocytosis

By similarity.

Post-translational modification: Proteolytically processed under normal cellular conditions. Cleavage either by alpha-secretase, beta-secretase or theta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF50, gamma-CTF57 and gamma-CTF59

By similarity.Proteolytically cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-3, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides

By similarity. Ref.17N- and O-glycosylated

By similarity.Phosphorylation in the C-terminal on tyrosine, threonine and serine residues is neuron-specific. Phosphorylation can affect APP processing, neuronal differentiation and interaction with other proteins. The Thr-743 phosphorylated form causes a conformational change which reduces binding of Fe65 family members

By similarity. Phosphorylation on Tyr-757 is required for SHC binding

By similarity. Ref.12 Ref.15Extracellular binding and reduction of copper, results in a corresponding oxidation of Cys-144 and Cys-158, and the formation of a disulfide bond

By similarity.Trophic-factor deprivation triggers the cleavage of surface APP by beta-secretase to release sAPP-beta which is further cleaved to release an N-terminal fragment of APP (N-APP)

By similarity. Ref.17Beta-amyloid peptides are degraded by IDE

By similarity.

Miscellaneous: Chelation of metal ions, notably copper, iron and zinc, can induce histidine-bridging between beta-amyloid molecules resulting in beta-amyloid-metal aggregates. Rat and mouse beta-amyloid peptides have an arginine residue substituted for the bridging histidine residue and are thus less capable of forming amyloid aggegates. Extracellular zinc-binding increases binding of heparin to APP and inhibits collagen-binding

By similarity.

Sequence similarities: Belongs to the APP family.Contains 1 BPTI/Kunitz inhibitor domain.
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 Research Articles on Abeta1-42    1. apoE influences sAbeta metabolism not through direct binding to sAbeta in solution but through its actions with other interacting receptors/transporters and cell surfaces
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with Abeta1-42 elisa kitDiseases associated with Abeta1-42 elisa kit
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4683 publications with Abeta1-42 and Nervous System Diseases
 Dementia Antibodies  >4530 publications with Abeta1-42 and Dementia
 Brain Diseases Antibodies  >4470 publications with Abeta1-42 and Brain Diseases
 Alzheimer Disease Antibodies  >4338 publications with Abeta1-42 and Alzheimer Disease
 Neurodegenerative Diseases Antibodies  >4102 publications with Abeta1-42 and Neurodegenerative Diseases
 Disease Models, Animal Antibodies  >1388 publications with Abeta1-42 and Disease Models, Animal
 Plaque, Amyloid Antibodies  >1012 publications with Abeta1-42 and Plaque, Amyloid
 Neoplasms Antibodies  >762 publications with Abeta1-42 and Neoplasms
 Inflammation Antibodies  >512 publications with Abeta1-42 and Inflammation
 Drug Toxicity Antibodies  >366 publications with Abeta1-42 and Drug Toxicity
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