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ApoB48 elisa kit :: Rat apolipoprotein B-48 (ApoB48) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS925341
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

apolipoprotein B-48 (ApoB48), ELISA Kit

★Popular Item★
 Also Known As   

Rat apolipoprotein B-48, ApoB48 ELISA Kit

 Product Synonym Names    Rat apolipoprotein B-48 (ApoB48) ELISA kit; apolipoprotein B-48 (ApoB48)
 Product Gene Name   

ApoB48 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS925341 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q7TMA5
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat ApoB48. No significant cross-reactivity or interference between rat ApoB48 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Detection Range    0.312 ug/ml - 20 ug/mL
 Sensitivity    <0.078 g/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ApoB48 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ApoB48 purchase    MBS925341 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the apolipoprotein B-48 (ApoB48) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ug/ml - 20 ug/mL in biological research samples containing ApoB48, with an estimated sensitivity of <0.078 g/mL. The ELISA analytical biochemical technique of the MBS925341 kit is based on ApoB48 antibody-ApoB48 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ApoB48 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ApoB48. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ApoB48 elisa kit

   Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ApoB48. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ApoB48. The competitive inhibition reaction is launched between with pre-coated ApoB48 and ApoB48 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ApoB48 in the samples. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ApoB48 elisa kit    ApoB48 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS925341. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ApoB48. It may not necessarily be applicable to this product.
 NCBI GI #    161783809
 NCBI GeneID    54225
 NCBI Accession #    NP_062160.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_019287.2 [Other Products]
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 UniProt Primary Accession #    Q7TMA5 [Other Products]
 UniProt Related Accession #    Q7TMA5 [Other Products]
 Molecular Weight    536,024 Da
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 NCBI Official Full Name    apolipoprotein B-100
 NCBI Official Synonym Full Names    apolipoprotein B
 NCBI Official Symbol    Apob [Similar Products]
 NCBI Official Synonym Symbols   
Aa1064; Ac1-060; ApoB-48; ApoB-100; Apo B-100
[Similar Products]
 NCBI Protein Information    apolipoprotein B-100; apolipoprotein B PI; apolipoprotein B-48; apolipoprotein B (including Ag(x) antigen)
 UniProt Protein Name    Apolipoprotein B-100
 UniProt Gene Name    Apob [Similar Products]
 UniProt Synonym Gene Names    Aa1064; Ac1-060; Apo B-100; Apo B-48 [Similar Products]
 UniProt Entry Name    APOB_RAT
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 NCBI Summary for ApoB48    This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-L and apoB-H. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the rat apoBL isoform is also found in rat liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-L protein is produced after RNA editing of the apoB-H transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ApoB48    APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted, signal peptide; Carrier; Secreted

Cellular Component: Golgi apparatus; extracellular space; chylomicron; cell soma; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; vesicle membrane; actin cytoskeleton

Molecular Function: heparin binding; lipid transporter activity; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding; lipid binding

Biological Process: lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to organic substance; response to selenium ion; triacylglycerol catabolic process; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; lipid transport; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; lipid metabolic process; triacylglycerol mobilization; lipid catabolic process
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 Research Articles on ApoB48    1. Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with ApoB48 elisa kitDiseases associated with ApoB48 elisa kit
 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 LDL-mediated Lipid Transport Pathway antibodies  LDL-mediated Lipid Transport Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
 Lipoprotein Metabolism Pathway antibodies  Lipoprotein Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3045 publications with ApoB48 and Cardiovascular Diseases
 Dyslipidemias Antibodies  >2688 publications with ApoB48 and Dyslipidemias
 Atherosclerosis Antibodies  >2289 publications with ApoB48 and Atherosclerosis
 Hyperlipidemias Antibodies  >2263 publications with ApoB48 and Hyperlipidemias
 Coronary Disease Antibodies  >1703 publications with ApoB48 and Coronary Disease
 Myocardial Ischemia Antibodies  >1561 publications with ApoB48 and Myocardial Ischemia
 Heart Diseases Antibodies  >1463 publications with ApoB48 and Heart Diseases
 Arteriosclerosis Antibodies  >1451 publications with ApoB48 and Arteriosclerosis
 Hypercholesterolemia Antibodies  >1327 publications with ApoB48 and Hypercholesterolemia
 Hyperlipoproteinemias Antibodies  >876 publications with ApoB48 and Hyperlipoproteinemias
Organs/Tissues associated with ApoB48 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2022 publications with ApoB48 and Liver
 Kidney Antibodies  >374 publications with ApoB48 and Kidney
 Small Intestine Antibodies  >204 publications with ApoB48 and Small Intestine
 Embryonic Tissue Antibodies  >52 publications with ApoB48 and Embryonic Tissue
 Spleen Antibodies  >50 publications with ApoB48 and Spleen
 Colon Antibodies  >31 publications with ApoB48 and Colon
 Pancreas Antibodies  >30 publications with ApoB48 and Pancreas
 Placenta Antibodies  >24 publications with ApoB48 and Placenta
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