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BDNF elisa kit :: Rat brain-derived neurotrophic factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703433
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,495 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,715 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

brain-derived neurotrophic factor (BDNF), ELISA Kit

 Also Known As   

Rat Brain derived neurotrophic facor, BDNF ELISA Kit

 Product Synonym Names    Rat Brain derived neurotrophic facor; BDNF ELISA Kit; MGC34632; neurotrophin; brain-derived neurotrophic factor
 Product Gene Name   

BDNF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS703433 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P23363
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat BDNF. No significant cross-reactivity or interference between rat BDNF and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.312 ng/ml -20 ng/ml.
 Sensitivity    The minimum detectable dose of rat BDNF is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of BDNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for BDNF purchase    MBS703433 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the brain-derived neurotrophic factor (BDNF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312 ng/ml -20 ng/ml in biological research samples containing BDNF, with an estimated sensitivity of The minimum detectable dose of rat BDNF is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS703433 kit is based on BDNF antibody-BDNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BDNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BDNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for BDNF elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BDNF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BDNF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of BDNF elisa kit    BDNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703433
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NCBI/Uniprot data below describe general gene information for BDNF. It may not necessarily be applicable to this product.
 NCBI GI #    397174764
 NCBI GeneID    24225
 NCBI Accession #    NP_001257559.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001270630.1 [Other Products]
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 UniProt Primary Accession #    P23363 [Other Products]
 UniProt Secondary Accession #    Q53YL8 [Other Products]
 UniProt Related Accession #    P23363 [Other Products]
 Molecular Weight    28,109 Da
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 NCBI Official Full Name    brain-derived neurotrophic factor isoform 1
 NCBI Official Synonym Full Names    brain-derived neurotrophic factor
 NCBI Official Symbol    Bdnf [Similar Products]
 NCBI Protein Information    brain-derived neurotrophic factor; brain derived neurotrophic factor; brain derived neurothrophic factor
 UniProt Protein Name    Brain-derived neurotrophic factor
 Protein Family    Brain-derived neurotrophic factor
 UniProt Gene Name    Bdnf [Similar Products]
 UniProt Synonym Gene Names    BDNF [Similar Products]
 UniProt Entry Name    BDNF_RAT
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 NCBI Summary for BDNF    plays a role in the development of hippocampal long term potentiation; involved in regulation of synaptic plasticity [RGD, Feb 2006]
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 UniProt Comments for BDNF    Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS
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 Research Articles on BDNF    1. Expression of BDNF is decreased in the amygdala during withdrwal from chronic ethanol exposure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with BDNF elisa kitPathways associated with BDNF elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with BDNF and FLNA
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Brain Derived Neurotrophic Factor Pathway antibodies  Brain Derived Neurotrophic Factor Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Cocaine Addiction Pathway antibodies  Cocaine Addiction Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
Diseases associated with BDNF elisa kitOrgans/Tissues associated with BDNF elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3553 publications with BDNF and Nervous System Diseases
 Brain Injuries Antibodies  >831 publications with BDNF and Brain Injuries
 Depressive Disorder Antibodies  >650 publications with BDNF and Depressive Disorder
 Schizophrenia Antibodies  >551 publications with BDNF and Schizophrenia
 Body Weight Antibodies  >544 publications with BDNF and Body Weight
 Brain Ischemia Antibodies  >542 publications with BDNF and Brain Ischemia
 Nerve Degeneration Antibodies  >535 publications with BDNF and Nerve Degeneration
 Cognition Disorders Antibodies  >530 publications with BDNF and Cognition Disorders
 Inflammation Antibodies  >525 publications with BDNF and Inflammation
 Memory Disorders Antibodies  >509 publications with BDNF and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with BDNF and Bone
 Connective Tissue Antibodies  >4 publications with BDNF and Connective Tissue
 Heart Antibodies  >3 publications with BDNF and Heart
 Muscle Antibodies  >3 publications with BDNF and Muscle
 Brain Antibodies  >3 publications with BDNF and Brain
 Vascular Antibodies  >2 publications with BDNF and Vascular
 Embryonic Tissue Antibodies  >2 publications with BDNF and Embryonic Tissue
 Ear Antibodies  >1 publications with BDNF and Ear
 Kidney Antibodies  >1 publications with BDNF and Kidney
 Umbilical Cord Antibodies  >1 publications with BDNF and Umbilical Cord
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