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CAMK2A elisa kit :: Rat calcium/calmodulin-dependent protein kinase II alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS702708
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
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 Product Name   

calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), ELISA Kit

★Popular Item★
 Also Known As   

Rat calcium/calmodulin-dependent protein kinase-II, CAMK 2 ELISA Kit

 Product Synonym Names    Rat calcium/calmodulin-dependent protein kinase-II; CAMK 2 ELISA Kit; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMKIINalpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-depende; calcium/calmodulin-dependent protein kinase II alpha
 Product Gene Name   

CAMK2A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS702708 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P11275
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat CAMK 2. No significant cross-reactivity or interference between rat CAMK 2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of rat CAMK 2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CAMK2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CAMK2A purchase    MBS702708 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing CAMK2A, with an estimated sensitivity of The minimum detectable dose of rat CAMK 2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS702708 kit is based on CAMK2A antibody-CAMK2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CAMK2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CAMK2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CAMK2A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAMK 2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAMK 2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAMK 2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAMK 2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CAMK2A elisa kit    CAMK2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS702708. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CAMK2A. It may not necessarily be applicable to this product.
 NCBI GI #    6978593
 NCBI GeneID    25400
 NCBI Accession #    NP_037052.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012920.1 [Other Products]
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 UniProt Primary Accession #    P11275 [Other Products]
 UniProt Related Accession #    P11275 [Other Products]
 Molecular Weight    54,115 Da
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 NCBI Official Full Name    calcium/calmodulin-dependent protein kinase type II subunit alpha
 NCBI Official Synonym Full Names    calcium/calmodulin-dependent protein kinase II alpha
 NCBI Official Symbol    Camk2a [Similar Products]
 NCBI Official Synonym Symbols   
PKCCD; PK2CDD
[Similar Products]
 NCBI Protein Information    calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
 UniProt Protein Name    Calcium/calmodulin-dependent protein kinase type II subunit alpha
 Protein Family    Calcium/calmodulin-dependent protein kinase
 UniProt Gene Name    Camk2a [Similar Products]
 UniProt Synonym Gene Names    CaM kinase II subunit alpha; CaMK-II subunit alpha [Similar Products]
 UniProt Entry Name    KCC2A_RAT
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 UniProt Comments for CAMK2A    Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.1 PublicationManual assertion based on experiment in:Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.1 PublicationManual assertion based on experiment in:Ref.6
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 Research Articles on CAMK2A    1. Increased Camk2a autophosphorylation was found in the medial prefrontal cortex and nucleus accumbens after last cocaine self-administration session.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CAMK2A elisa kitPathways associated with CAMK2A elisa kit
 Reference Product  PubMed Publications
 Camk2b elisa kit  >7 publications with CAMK2A and Camk2b
 Arc elisa kit  >2 publications with CAMK2A and Arc
 Grin2a elisa kit  >1 publications with CAMK2A and Grin2a
 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
Diseases associated with CAMK2A elisa kitOrgans/Tissues associated with CAMK2A elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >48 publications with CAMK2A and Nervous System Diseases
 Brain Diseases Antibodies  >32 publications with CAMK2A and Brain Diseases
 Seizures Antibodies  >8 publications with CAMK2A and Seizures
 Pain Antibodies  >8 publications with CAMK2A and Pain
 Memory Disorders Antibodies  >6 publications with CAMK2A and Memory Disorders
 Movement Disorders Antibodies  >6 publications with CAMK2A and Movement Disorders
 Learning Disorders Antibodies  >5 publications with CAMK2A and Learning Disorders
 Inflammation Antibodies  >5 publications with CAMK2A and Inflammation
 Cognition Disorders Antibodies  >4 publications with CAMK2A and Cognition Disorders
 Necrosis Antibodies  >3 publications with CAMK2A and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >107 publications with CAMK2A and Brain
 Muscle Antibodies  >4 publications with CAMK2A and Muscle
 Eye Antibodies  >4 publications with CAMK2A and Eye
 Kidney Antibodies  >4 publications with CAMK2A and Kidney
 Embryonic Tissue Antibodies  >3 publications with CAMK2A and Embryonic Tissue
 Heart Antibodies  >2 publications with CAMK2A and Heart
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