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CPB1 elisa kit :: Rat carboxypeptidase B1 (tissue) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS908180
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

carboxypeptidase B1 (tissue) (CPB1), ELISA Kit

★Popular Item★
 Also Known As   

Rat Carboxypeptidase B, CPB1 ELISA Kit

 Product Synonym Names    Rat Carboxypeptidase B (CPB1) ELISA kit; CPB; DKFZp779K1333; PASP; PCPB; pancreas-specific protein; pancreatic carboxypeptidase B1; procarboxypeptidase B; carboxypeptidase B1 (tissue)
 Product Gene Name   

CPB1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS908180 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P19223
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat CPB1. No significant cross-reactivity or interference between rat CPB1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates.
 Assay Type    Sandwich
 Detection Range    12.5 ng/ml-800 ng/ml.
 Sensitivity    Typically less than 3.12 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CPB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CPB1 purchase    MBS908180 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the carboxypeptidase B1 (tissue) (CPB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 ng/ml-800 ng/ml in biological research samples containing CPB1, with an estimated sensitivity of Typically less than 3.12 ng/ml. The ELISA analytical biochemical technique of the MBS908180 kit is based on CPB1 antibody-CPB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CPB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CPB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CPB1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CPB1 elisa kit    CPB1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS908180. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CPB1. It may not necessarily be applicable to this product.
 NCBI GI #    6978697
 NCBI GeneID    24271
 NCBI Accession #    NP_036665.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012533.1 [Other Products]
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 UniProt Primary Accession #    P19223 [Other Products]
 UniProt Related Accession #    P19223 [Other Products]
 Molecular Weight    47,515 Da
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 NCBI Official Full Name    carboxypeptidase B
 NCBI Official Synonym Full Names    carboxypeptidase B1 (tissue)
 NCBI Official Symbol    Cpb1 [Similar Products]
 NCBI Official Synonym Symbols   
Cpb
[Similar Products]
 NCBI Protein Information    carboxypeptidase B; pancreatic carboxypeptidase B1
 UniProt Protein Name    Carboxypeptidase B
 UniProt Gene Name    Cpb1 [Similar Products]
 UniProt Synonym Gene Names    Cpb [Similar Products]
 UniProt Entry Name    CBPB1_RAT
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 NCBI Summary for CPB1    putative carboxypeptidase B protein [RGD, Feb 2006]
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 UniProt Comments for CPB1    CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

Protein type: Protease; Secreted; EC 3.4.17.2; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: zinc ion binding; carboxypeptidase activity; metallocarboxypeptidase activity

Biological Process: proteolysis
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 Research Articles on CPB1    1. Mutants C383G, C383A had higher k(cat)/K(m) values which indicated increased catalytic abilities. And both had higher thermal stability.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CPB1 elisa kitDiseases associated with CPB1 elisa kit
 Products by Pathway  Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with CPB1 and Neoplasms
 Immune System Diseases Antibodies  >2 publications with CPB1 and Immune System Diseases
 Prostatic Diseases Antibodies  >2 publications with CPB1 and Prostatic Diseases
 Prostatic Hyperplasia Antibodies  >1 publications with CPB1 and Prostatic Hyperplasia
 Liver Neoplasms Antibodies  >1 publications with CPB1 and Liver Neoplasms
 Hyperplasia Antibodies  >1 publications with CPB1 and Hyperplasia
 Liver Neoplasms, Experimental Antibodies  >1 publications with CPB1 and Liver Neoplasms, Experimental
Organs/Tissues associated with CPB1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >18 publications with CPB1 and Pancreas
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