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COL2A1 elisa kit :: Rat collagen, type II, alpha 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS907543
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

collagen, type II, alpha 1 (COL2A1), ELISA Kit

 Also Known As   

Rat Collagen alpha-1 (II) chain, COL2A1 ELISA Kit

 Product Synonym Names    Rat Collagen alpha-1 (II) chain (COL2A1) ELISA kit; ANFH; AOM; COL11A3; MGC131516; SEDC; cartilage collagen; chondrocalcin; collagen II; alpha-1 polypeptide; collagen; type II; alpha 1
 Product Gene Name   

COL2A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS907543 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P05539
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat COL2A1. No significant cross-reactivity or interference between rat COL2A1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml -2000 pg/ml.
 Sensitivity    The minimum detectable dose of rat COL2A1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of COL2A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for COL2A1 purchase    MBS907543 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the collagen, type II, alpha 1 (COL2A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml -2000 pg/ml in biological research samples containing COL2A1, with an estimated sensitivity of The minimum detectable dose of rat COL2A1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS907543 kit is based on COL2A1 antibody-COL2A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL2A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL2A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for COL2A1 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL2A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of COL2A1 elisa kit    COL2A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS907543. Click to request current manual
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NCBI/Uniprot data below describe general gene information for COL2A1. It may not necessarily be applicable to this product.
 NCBI GI #    6978677
 NCBI GeneID    25412
 NCBI Accession #    NP_037061.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012929.1 [Other Products]
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 UniProt Primary Accession #    P05539 [Other Products]
 UniProt Secondary Accession #    Q63123; Q63565; Q78DY3 [Other Products]
 UniProt Related Accession #    P05539 [Other Products]
 Molecular Weight    134,570 Da
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 NCBI Official Full Name    collagen alpha-1(II) chain
 NCBI Official Synonym Full Names    collagen, type II, alpha 1
 NCBI Official Symbol    Col2a1 [Similar Products]
 NCBI Official Synonym Symbols   
CG2A1A; COLLII
[Similar Products]
 NCBI Protein Information    collagen alpha-1(II) chain; collagen alpha-1(II) chain; Procollagen II alpha 1; alpha-1 type II collagen; procollagen, type II, alpha 1; collagen type II (Col2A1) gene, enhancer region
 UniProt Protein Name    Collagen alpha-1(II) chain
 UniProt Synonym Protein Names   
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
 UniProt Gene Name    Col2a1 [Similar Products]
 UniProt Entry Name    CO2A1_RAT
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 NCBI Summary for COL2A1    structural component of the cartilage extracellular matrix [RGD, Feb 2006]
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 UniProt Comments for COL2A1    Function: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
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 Research Articles on COL2A1    1. Screening of the Col2a1 gene, and enhancer domain, for the sequence encoded regulation of its expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with COL2A1 elisa kitDiseases associated with COL2A1 elisa kit
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Disease Name  Pubmed Publications
 Osteoarthritis Antibodies  >148 publications with COL2A1 and Osteoarthritis
 Osteochondrodysplasias Antibodies  >123 publications with COL2A1 and Osteochondrodysplasias
 Retinal Detachment Antibodies  >46 publications with COL2A1 and Retinal Detachment
 Myopia Antibodies  >40 publications with COL2A1 and Myopia
 Cleft Palate Antibodies  >37 publications with COL2A1 and Cleft Palate
 Craniofacial Abnormalities Antibodies  >34 publications with COL2A1 and Craniofacial Abnormalities
 Nervous System Diseases Antibodies  >29 publications with COL2A1 and Nervous System Diseases
 Kniest dysplasia Antibodies  >24 publications with COL2A1 and Kniest dysplasia
 Neoplasms Antibodies  >23 publications with COL2A1 and Neoplasms
 Necrosis Antibodies  >20 publications with COL2A1 and Necrosis
Organs/Tissues associated with COL2A1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Connective Tissue Antibodies  >224 publications with COL2A1 and Connective Tissue
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