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CRH elisa kit :: Rat corticotropin releasing hormone ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703710
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
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 Product Name   

corticotropin releasing hormone (CRH), ELISA Kit

★Popular Item★
 Also Known As   

Rat corticotropin releasing hormone, CRH ELISA Kit

 Product Synonym Names    Rat corticotropin releasing hormone; CRH ELISA Kit; CRF; corticotropin-releasing factor; corticotropin releasing hormone
 Product Gene Name   

CRH elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703710 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P01143
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat CRH. No significant cross-reactivity or interference between rat CRH and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Competitive
 Detection Range    1.25 ng/ml -20 ng/ml
 Sensitivity    Typically less than 1.25 ng/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CRH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CRH purchase    MBS703710 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the corticotropin releasing hormone (CRH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.25 ng/ml -20 ng/ml in biological research samples containing CRH, with an estimated sensitivity of Typically less than 1.25 ng/ml. The ELISA analytical biochemical technique of the MBS703710 kit is based on CRH antibody-CRH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CRH elisa kit

   Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRH. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated CRH. A competitive inhibition reaction is launched between CRH (Standards or samples) and Biotin-conjugated CRH with the pre-coated antibody specific for CRH. The more amount of CRH in samples, the less antibody bound by Biotin-conjugated CRH. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of CRH in the sample. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of CRH elisa kit    CRH elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703710. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CRH. It may not necessarily be applicable to this product.
 NCBI GI #    13591920
 NCBI GeneID    81648
 NCBI Accession #    NP_112281.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031019.1 [Other Products]
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 UniProt Primary Accession #    P01143 [Other Products]
 UniProt Related Accession #    P01143 [Other Products]
 Molecular Weight    20,680 Da
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 NCBI Official Full Name    corticoliberin
 NCBI Official Synonym Full Names    corticotropin releasing hormone
 NCBI Official Symbol    Crh [Similar Products]
 NCBI Official Synonym Symbols   
CRF
[Similar Products]
 NCBI Protein Information    corticoliberin; corticotropin-releasing factor; corticotropin-releasing hormone
 UniProt Protein Name    Corticoliberin
 UniProt Synonym Protein Names   
Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone
 Protein Family    Corticoliberin
 UniProt Gene Name    Crh [Similar Products]
 UniProt Synonym Gene Names    CRF [Similar Products]
 UniProt Entry Name    CRF_RAT
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 NCBI Summary for CRH    binds CRH receptors and mediates neuroendocrine stress response; may play a role in the consolidation of long term memory [RGD, Feb 2006]
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 UniProt Comments for CRH    Function: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.1 PublicationManual assertion based on experiment in:Ref.3
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 Research Articles on CRH    1. evidence is provided that high-mobility group box 1 and corticotropin-releasing factor release are critical for the chronic ethanol withdrawal induction of selected brain cytokine messenger RNAs
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CRH elisa kitPathways associated with CRH elisa kit
 Reference Product  PubMed Publications
 Pomc elisa kit  >2194 publications with CRH and Pomc
 Fos elisa kit  >198 publications with CRH and Fos
 Npy elisa kit  >179 publications with CRH and Npy
 Crhr1 elisa kit  >165 publications with CRH and Crhr1
 Ucn elisa kit  >91 publications with CRH and Ucn
 Crhr2 elisa kit  >75 publications with CRH and Crhr2
 Crhbp elisa kit  >30 publications with CRH and Crhbp
 Nr3c1 elisa kit  >15 publications with CRH and Nr3c1
 Il6 elisa kit  >8 publications with CRH and Il6
 Ghrl elisa kit  >2 publications with CRH and Ghrl
 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Serotonin And Anxiety Pathway antibodies  Serotonin And Anxiety Pathway Diagram
Diseases associated with CRH elisa kitOrgans/Tissues associated with CRH elisa kit
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >663 publications with CRH and Mental Disorders
 Nervous System Diseases Antibodies  >641 publications with CRH and Nervous System Diseases
 Brain Diseases Antibodies  >504 publications with CRH and Brain Diseases
 Cushing Syndrome Antibodies  >368 publications with CRH and Cushing Syndrome
 Depressive Disorder Antibodies  >325 publications with CRH and Depressive Disorder
 Inflammation Antibodies  >246 publications with CRH and Inflammation
 Anxiety Disorders Antibodies  >226 publications with CRH and Anxiety Disorders
 Necrosis Antibodies  >133 publications with CRH and Necrosis
 Hypertension Antibodies  >118 publications with CRH and Hypertension
 Pain Antibodies  >99 publications with CRH and Pain
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with CRH and Bone
 Connective Tissue Antibodies  >4 publications with CRH and Connective Tissue
 Heart Antibodies  >3 publications with CRH and Heart
 Muscle Antibodies  >3 publications with CRH and Muscle
 Brain Antibodies  >3 publications with CRH and Brain
 Vascular Antibodies  >2 publications with CRH and Vascular
 Embryonic Tissue Antibodies  >2 publications with CRH and Embryonic Tissue
 Ear Antibodies  >1 publications with CRH and Ear
 Kidney Antibodies  >1 publications with CRH and Kidney
 Umbilical Cord Antibodies  >1 publications with CRH and Umbilical Cord
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