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CBS elisa kit :: Rat cystathionine-beta-synthase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS703393
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

cystathionine-beta-synthase (CBS), ELISA Kit

 Also Known As   

Rat cystathionine beta-synthase, CBS ELISA Kit

 Product Synonym Names    Rat cystathionine beta-synthase (CBS) ELISA Kit; HIP4; OTTHUMP00000109415; OTTHUMP00000109416; OTTHUMP00000109418; beta-thionase; cystathionine beta-synthase; methylcysteine synthase; serine sulfhydrase; cystathionine-beta-synthase
 Product Gene Name   

CBS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS703393 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q9N0V7
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat CBS. No significant cross-reactivity or interference between Rat CBS and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.8 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Biosynthesis/Metabolism
 Protein Biological Process 2    Amino-acid biosynthesis and metabolism
 Protein Biological Process 3    Amino-acid biosynthesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CBS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CBS purchase    MBS703393 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cystathionine-beta-synthase (CBS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing CBS, with an estimated sensitivity of 7.8 pg/ml. The ELISA analytical biochemical technique of the MBS703393 kit is based on CBS antibody-CBS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CBS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CBS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for CBS elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CBS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CBS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CBS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CBS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of CBS elisa kit    CBS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS703393. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CBS. It may not necessarily be applicable to this product.
 NCBI GI #    126723756
 NCBI GeneID    100008766
 NCBI Accession #    NP_001075551.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001082082.2 [Other Products]
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 UniProt Primary Accession #    Q9N0V7 [Other Products]
 UniProt Related Accession #    Q9N0V7 [Other Products]
 Molecular Weight    60,212 Da
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 NCBI Official Full Name    cystathionine beta-synthase
 NCBI Official Symbol    CBS [Similar Products]
 NCBI Protein Information    cystathionine beta-synthase; beta-thionase; serine sulfhydrase
 UniProt Protein Name    Cystathionine beta-synthase
 UniProt Synonym Protein Names   
Beta-thionase; Serine sulfhydrase
 Protein Family    Cystathionine beta-synthase
 UniProt Gene Name    CBS [Similar Products]
 UniProt Entry Name    CBS_RABIT
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 UniProt Comments for CBS    Function: Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury

Catalytic activity: L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactor: Pyridoxal phosphate
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with CBS elisa kitPathways associated with CBS elisa kit
 Reference Product  PubMed Publications
 MTR elisa kit  >76 publications with CBS and MTR
 BHMT elisa kit  >36 publications with CBS and BHMT
 SHMT1 elisa kit  >20 publications with CBS and SHMT1
 CTH elisa kit  >14 publications with CBS and CTH
 PSPH elisa kit  >1 publications with CBS and PSPH
 SHMT2 elisa kit  >1 publications with CBS and SHMT2
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Diseases associated with CBS elisa kitOrgans/Tissues associated with CBS elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >692 publications with CBS and Nervous System Diseases
 Brain Diseases Antibodies  >467 publications with CBS and Brain Diseases
 Hyperhomocysteinemia Antibodies  >344 publications with CBS and Hyperhomocysteinemia
 Cardiovascular Diseases Antibodies  >337 publications with CBS and Cardiovascular Diseases
 Homocystinuria Antibodies  >181 publications with CBS and Homocystinuria
 Malnutrition Antibodies  >163 publications with CBS and Malnutrition
 Inflammation Antibodies  >101 publications with CBS and Inflammation
 Congenital Abnormalities Antibodies  >89 publications with CBS and Congenital Abnormalities
 Hypertension Antibodies  >65 publications with CBS and Hypertension
 Liver Diseases Antibodies  >64 publications with CBS and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with CBS and Bone
 Connective Tissue Antibodies  >4 publications with CBS and Connective Tissue
 Heart Antibodies  >3 publications with CBS and Heart
 Muscle Antibodies  >3 publications with CBS and Muscle
 Brain Antibodies  >3 publications with CBS and Brain
 Vascular Antibodies  >2 publications with CBS and Vascular
 Embryonic Tissue Antibodies  >2 publications with CBS and Embryonic Tissue
 Ear Antibodies  >1 publications with CBS and Ear
 Kidney Antibodies  >1 publications with CBS and Kidney
 Umbilical Cord Antibodies  >1 publications with CBS and Umbilical Cord
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