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FNDC5 elisa kit :: Rat fibronectin type III domain containing 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS912294
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

fibronectin type III domain containing 5 (FNDC5), ELISA Kit

 Also Known As   

Rat Fibronectin type III domain-containing protein 5, FNDC5 ELISA Kit

 Product Synonym Names    Rat Fibronectin type III domain-containing protein 5 (FNDC5) ELISA kit; FRCP2; ; fibronectin type III domain containing 5
 Product Gene Name   

FNDC5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS912294 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8K3V5
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat FNDC5. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Detection Range    0.625 ng/ml-40 ng/ml
 Sensitivity    0.156 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of FNDC5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FNDC5 purchase    MBS912294 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fibronectin type III domain containing 5 (FNDC5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing FNDC5, with an estimated sensitivity of 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS912294 kit is based on FNDC5 antibody-FNDC5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FNDC5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FNDC5. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for FNDC5 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FNDC5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FNDC5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FNDC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FNDC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of FNDC5 elisa kit    FNDC5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS912294
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NCBI/Uniprot data below describe general gene information for FNDC5. It may not necessarily be applicable to this product.
 NCBI GI #    401664562
 NCBI GeneID    260327
 NCBI Accession #    NP_001257910.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001270981.1 [Other Products]
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 UniProt Primary Accession #    Q8K3V5 [Other Products]
 UniProt Related Accession #    Q8K3V5 [Other Products]
 Molecular Weight    23,321 Da
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 NCBI Official Full Name    fibronectin type III domain-containing protein 5
 NCBI Official Synonym Full Names    fibronectin type III domain containing 5
 NCBI Official Symbol    Fndc5 [Similar Products]
 NCBI Protein Information    fibronectin type III domain-containing protein 5; peroxisomal protein
 UniProt Protein Name    Fibronectin type III domain-containing protein 5
 Protein Family    Fibronectin type III domain-containing protein
 UniProt Gene Name    Fndc5 [Similar Products]
 UniProt Entry Name    FNDC5_RAT
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 NCBI Summary for FNDC5    mouse homolog is linked to myoblast differentiation and development [RGD, Feb 2006]
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 UniProt Comments for FNDC5    FNDC5: Irisin: mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: peroxisomal membrane; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region

Molecular Function: hormone activity

Biological Process: response to muscle activity
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 Research Articles on FNDC5    1. This immunohistochemical results demonstrate the expression of irisin immunoreactivity in three types of cells: skeletal, cardiac muscle, and Purkinje cells of the cerebellum.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FNDC5 elisa kitPathways associated with FNDC5 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with FNDC5 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with FNDC5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >3 publications with FNDC5 and Muscle
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