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HSPG2 elisa kit :: Rat heparan sulfate proteoglycan 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS706413
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

heparan sulfate proteoglycan 2 (HSPG2), ELISA Kit

 Also Known As   

Rat heparan sulfate proteoglycan 2, HSPG2 ELISA Kit

 Product Synonym Names    Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit; RP11-132G19.2; PLC; PRCAN; SJA; SJS; SJS1; Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia) ; endorepellin (domain V region) ; heparan sulfate proteoglycan of basement membrane; perlecan proteogly; heparan sulfate proteoglycan 2
 Product Gene Name   

HSPG2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS706413 Testing Data    Testing Data PDF
 OMIM    255800
 3D Structure    ModBase 3D Structure for P98160
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 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat HSPG2. No significant cross-reactivity or interference between rat HSPG2 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml.
 Sensitivity    The minimum detectable dose of rat HSPG2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HSPG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HSPG2 purchase    MBS706413 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the heparan sulfate proteoglycan 2 (HSPG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing HSPG2, with an estimated sensitivity of The minimum detectable dose of rat HSPG2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS706413 kit is based on HSPG2 antibody-HSPG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for HSPG2 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HSPG2 elisa kit    HSPG2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS706413. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HSPG2. It may not necessarily be applicable to this product.
 NCBI GI #    629266065
 NCBI GeneID    3339
 NCBI Accession #    NP_001278789.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001291860.1 [Other Products]
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 UniProt Primary Accession #    P98160 [Other Products]
 UniProt Secondary Accession #    Q16287; Q5SZI3; Q9H3V5 [Other Products]
 UniProt Related Accession #    P98160 [Other Products]
 Molecular Weight    468,830 Da
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 NCBI Official Full Name    basement membrane-specific heparan sulfate proteoglycan core protein isoform a
 NCBI Official Synonym Full Names    heparan sulfate proteoglycan 2
 NCBI Official Symbol    HSPG2 [Similar Products]
 NCBI Official Synonym Symbols   
PLC; SJA; SJS; HSPG; SJS1; PRCAN
[Similar Products]
 NCBI Protein Information    basement membrane-specific heparan sulfate proteoglycan core protein; perlecan proteoglycan; endorepellin (domain V region)
 UniProt Protein Name    Basement membrane-specific heparan sulfate proteoglycan core protein
 UniProt Synonym Protein Names   
Perlecan; PLC
 Protein Family    Small heat shock protein
 UniProt Gene Name    HSPG2 [Similar Products]
 UniProt Synonym Gene Names    HSPG; PLC [Similar Products]
 UniProt Entry Name    PGBM_HUMAN
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 NCBI Summary for HSPG2    This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
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 UniProt Comments for HSPG2    Function: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.5 PublicationsManual assertion based on experiment in:Ref.9
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 Research Articles on HSPG2    1. role for perlecan in chondrogenic and osteogenic events which drive discal development and ossification of the vertebral bodies.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HSPG2 elisa kitPathways associated with HSPG2 elisa kit
 Reference Product  PubMed Publications
 SDC2 elisa kit  >2 publications with HSPG2 and SDC2
 HPSE elisa kit  >1 publications with HSPG2 and HPSE
 BGN elisa kit  >1 publications with HSPG2 and BGN
 APP elisa kit  >1 publications with HSPG2 and APP
 Products by Pathway  Pathway Diagram
 A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies  A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Chylomicron-mediated Lipid Transport Pathway antibodies  Chylomicron-mediated Lipid Transport Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
Diseases associated with HSPG2 elisa kitOrgans/Tissues associated with HSPG2 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >17 publications with HSPG2 and Nervous System Diseases
 Mental Disorders Antibodies  >9 publications with HSPG2 and Mental Disorders
 Osteochondrodysplasias Antibodies  >9 publications with HSPG2 and Osteochondrodysplasias
 Disease Models, Animal Antibodies  >6 publications with HSPG2 and Disease Models, Animal
 Dyssegmental dysplasia Antibodies  >6 publications with HSPG2 and Dyssegmental dysplasia
 Kidney Diseases Antibodies  >5 publications with HSPG2 and Kidney Diseases
 Dementia Antibodies  >5 publications with HSPG2 and Dementia
 Alzheimer Disease Antibodies  >5 publications with HSPG2 and Alzheimer Disease
 Movement Disorders Antibodies  >5 publications with HSPG2 and Movement Disorders
 Congenital Abnormalities Antibodies  >4 publications with HSPG2 and Congenital Abnormalities
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >7 publications with HSPG2 and Bone
 Muscle Antibodies  >6 publications with HSPG2 and Muscle
 Embryonic Tissue Antibodies  >6 publications with HSPG2 and Embryonic Tissue
 Connective Tissue Antibodies  >5 publications with HSPG2 and Connective Tissue
 Vascular Antibodies  >5 publications with HSPG2 and Vascular
 Blood Antibodies  >5 publications with HSPG2 and Blood
 Eye Antibodies  >4 publications with HSPG2 and Eye
 Brain Antibodies  >4 publications with HSPG2 and Brain
 Kidney Antibodies  >3 publications with HSPG2 and Kidney
 Ovary Antibodies  >3 publications with HSPG2 and Ovary
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