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HAMP elisa kit :: Rat hepcidin antimicrobial peptide ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910836
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

hepcidin antimicrobial peptide (HAMP), ELISA Kit

★Popular Item★
 Also Known As   

Rat Hepcidin, HAMP ELISA Kit

 Product Synonym Names    Rat Hepcidin (HAMP) ELISA kit; HEPC; HEPCIDIN; HFE2B; LEAP-1; LEAP1; PLTR; liver-expressed antimicrobial peptide; putative liver tumor regressor; hepcidin antimicrobial peptide
 Product Gene Name   

HAMP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS910836 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q99MH3
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat HAMP. No significant cross-reactivity or interference between Rat HAMP and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    0.156 ng/ml-10 ng/ml
 Sensitivity    0.039 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HAMP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HAMP purchase    MBS910836 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hepcidin antimicrobial peptide (HAMP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156 ng/ml-10 ng/ml in biological research samples containing HAMP, with an estimated sensitivity of 0.039 ng/ml. The ELISA analytical biochemical technique of the MBS910836 kit is based on HAMP antibody-HAMP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HAMP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HAMP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HAMP elisa kit

   For Samples:For the quantitative determination of rat hepcidin (HAMP) concentrations in serum, plasma, cell culture supernates, tissue homogenates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HAMP elisa kit    HAMP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910836. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HAMP. It may not necessarily be applicable to this product.
 NCBI GI #    16758218
 NCBI GeneID    84604
 NCBI Accession #    NP_445921.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_053469.1 [Other Products]
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 UniProt Primary Accession #    Q99MH3 [Other Products]
 UniProt Related Accession #    Q99MH3 [Other Products]
 Molecular Weight    9,286 Da
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 NCBI Official Full Name    hepcidin
 NCBI Official Synonym Full Names    hepcidin antimicrobial peptide
 NCBI Official Symbol    Hamp [Similar Products]
 NCBI Official Synonym Symbols   
Hepc
[Similar Products]
 NCBI Protein Information    hepcidin
 UniProt Protein Name    Hepcidin
 Protein Family    Hepcidin
 UniProt Gene Name    Hamp [Similar Products]
 UniProt Synonym Gene Names    Hepc [Similar Products]
 UniProt Entry Name    HEPC_RAT
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 NCBI Summary for HAMP    may play a role in the regulation of iron metabolism [RGD, Feb 2006]
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 UniProt Comments for HAMP    HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; apical cortex; cell; cytoplasm; extracellular region; nucleus

Molecular Function: copper ion binding; hormone activity

Biological Process: iron ion homeostasis; cellular iron ion homeostasis; negative regulation of inflammatory response; myeloid cell homeostasis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; negative regulation of bone resorption; JAK-STAT cascade; defense response to fungus; positive regulation of macrophage activation
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 Research Articles on HAMP    1. The data presented here establish for the first time a causal association between inflammation and iron accumulation in brain cells, probably promoted by changes in DMT1 and FPN1 expression and mediated in part by hepcidin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HAMP elisa kitDiseases associated with HAMP elisa kit
 Reference Product  PubMed Publications
 Tfr2 elisa kit  >130 publications with HAMP and Tfr2
 Hfe2 elisa kit  >113 publications with HAMP and Hfe2
 Stat3 elisa kit  >52 publications with HAMP and Stat3
 Slc40a1 elisa kit  >41 publications with HAMP and Slc40a1
 Il6 elisa kit  >32 publications with HAMP and Il6
 Tlr4 elisa kit  >10 publications with HAMP and Tlr4
 Heph elisa kit  >9 publications with HAMP and Heph
 Epor elisa kit  >5 publications with HAMP and Epor
 Slc11a2 elisa kit  >5 publications with HAMP and Slc11a2
 Disease Name  Pubmed Publications
 Anemia Antibodies  >667 publications with HAMP and Anemia
 Iron Overload Antibodies  >627 publications with HAMP and Iron Overload
 Inflammation Antibodies  >491 publications with HAMP and Inflammation
 Hemochromatosis Antibodies  >425 publications with HAMP and Hemochromatosis
 Liver Diseases Antibodies  >245 publications with HAMP and Liver Diseases
 Kidney Diseases Antibodies  >139 publications with HAMP and Kidney Diseases
 Disease Models, Animal Antibodies  >113 publications with HAMP and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >77 publications with HAMP and Cardiovascular Diseases
 Necrosis Antibodies  >70 publications with HAMP and Necrosis
 beta-Thalassemia Antibodies  >63 publications with HAMP and beta-Thalassemia
Organs/Tissues associated with HAMP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >65 publications with HAMP and Liver
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