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HIF1A elisa kit :: Rat hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704416
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), ELISA Kit

★Popular Item★
 Also Known As   

Rat hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

 Product Synonym Names    Rat hypoxia-inducible factor 1alpha; HIF-1alpha ELISA Kit; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; hypoxia-inducible factor 1; alpha; hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor)
 Product Gene Name   

HIF1A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704416 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35800
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of rat HIF-1alpha. No significant cross-reactivity or interference between rat HIF-1alpha and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    3.12 pg/ml -200 pg/ml.
 Sensitivity    The minimum detectable dose of rat HIF-1alpha is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HIF1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HIF1A purchase    MBS704416 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12 pg/ml -200 pg/ml in biological research samples containing HIF1A, with an estimated sensitivity of The minimum detectable dose of rat HIF-1alpha is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS704416 kit is based on HIF1A antibody-HIF1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HIF1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HIF1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HIF1A elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of HIF1A elisa kit    HIF1A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704416. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HIF1A. It may not necessarily be applicable to this product.
 NCBI GI #    13242249
 NCBI GeneID    29560
 NCBI Accession #    NP_077335.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_024359.1 [Other Products]
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 UniProt Primary Accession #    O35800 [Other Products]
 UniProt Secondary Accession #    Q9WTU9 [Other Products]
 UniProt Related Accession #    O35800 [Other Products]
 Molecular Weight    92,319 Da
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 NCBI Official Full Name    hypoxia-inducible factor 1-alpha
 NCBI Official Synonym Full Names    hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
 NCBI Official Symbol    Hif1a [Similar Products]
 NCBI Official Synonym Symbols   
MOP1
[Similar Products]
 NCBI Protein Information    hypoxia-inducible factor 1-alpha; HIF1 alpha; HIF1-alpha; HIF-1 alpha; HIF-1-alpha; hypoxia-inducible factor 1 alpha; hypoxia inducible factor 1, alpha subunit
 UniProt Protein Name    Hypoxia-inducible factor 1-alpha
 Protein Family    Hypoxia-inducible factor
 UniProt Gene Name    Hif1a [Similar Products]
 UniProt Synonym Gene Names    HIF-1-alpha; HIF1-alpha [Similar Products]
 UniProt Entry Name    HIF1A_RAT
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 NCBI Summary for HIF1A    regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006]
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 UniProt Comments for HIF1A    Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia
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 Research Articles on HIF1A    1. The present results suggest that HIF-1alpha may mediate iron accumulation in hypoxic microglia
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HIF1A elisa kitPathways associated with HIF1A elisa kit
 Reference Product  PubMed Publications
 Vhl elisa kit  >515 publications with HIF1A and Vhl
 Vegfa elisa kit  >492 publications with HIF1A and Vegfa
 Arnt elisa kit  >282 publications with HIF1A and Arnt
 Egln1 elisa kit  >132 publications with HIF1A and Egln1
 Egln3 elisa kit  >66 publications with HIF1A and Egln3
 Hif1an elisa kit  >58 publications with HIF1A and Hif1an
 Ep300 elisa kit  >26 publications with HIF1A and Ep300
 Egln2 elisa kit  >18 publications with HIF1A and Egln2
 Crebbp elisa kit  >14 publications with HIF1A and Crebbp
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Proteoglycans In Cancer Pathway antibodies  Proteoglycans In Cancer Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Renal Cell Carcinoma Pathway antibodies  Renal Cell Carcinoma Pathway Diagram
 Thyroid Hormone Signaling Pathway antibodies  Thyroid Hormone Signaling Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram
 MTOR Signaling Pathway antibodies  MTOR Signaling Pathway Diagram
Diseases associated with HIF1A elisa kitOrgans/Tissues associated with HIF1A elisa kit
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1371 publications with HIF1A and Carcinoma
 Nervous System Diseases Antibodies  >659 publications with HIF1A and Nervous System Diseases
 Inflammation Antibodies  >516 publications with HIF1A and Inflammation
 Brain Diseases Antibodies  >482 publications with HIF1A and Brain Diseases
 Kidney Diseases Antibodies  >428 publications with HIF1A and Kidney Diseases
 Breast Neoplasms Antibodies  >426 publications with HIF1A and Breast Neoplasms
 Lung Diseases Antibodies  >420 publications with HIF1A and Lung Diseases
 Necrosis Antibodies  >372 publications with HIF1A and Necrosis
 Liver Diseases Antibodies  >363 publications with HIF1A and Liver Diseases
 Neoplasms, Experimental Antibodies  >340 publications with HIF1A and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >502 publications with HIF1A and Kidney
 Lung Antibodies  >433 publications with HIF1A and Lung
 Embryonic Tissue Antibodies  >229 publications with HIF1A and Embryonic Tissue
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