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Lp-PLA2 elisa kit :: Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS704213
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $590 +1 FREE 8GB USB
96-Strip-Wells  /  $855 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
 
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 Product Name   

lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit

 Also Known As   

Rat lipoprotein-associated phospholipase A2, Lp-PLA2 ELISA Kit

 Product Synonym Names    Rat lipoprotein-associated phospholipase A2; Lp-PLA2 ELISA Kit; lipoprotein-associated phospholipase A2; Lp-PLA2
 Product Gene Name   

Lp-PLA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS704213 Testing Data    Testing Data PDF
 Species Reactivity    Rat
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 Detection Range    7.8 ng/ml-500 ng/ml
 Sensitivity    2 ng/ml
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Lp-PLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lp-PLA2 purchase    MBS704213 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lipoprotein-associated phospholipase A2, Lp-PLA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 7.8 ng/ml-500 ng/ml in biological research samples containing Lp-PLA2, with an estimated sensitivity of 2 ng/ml. The ELISA analytical biochemical technique of the MBS704213 kit is based on Lp-PLA2 antibody-Lp-PLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lp-PLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lp-PLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Lp-PLA2 elisa kit

   Introduction: Lipoprotein-associated phospholipase A2 (Lp-PLA2, also known as platelet activating factor acetyl-hydrolase), is a relatively recent marker that has emerged as a major, independent predictor of CHD. Lp-PLA2 is an active phospholipase that circulates in plasma bound to LDL, and it appears to act almost exclusively on oxidized LDL to generate lysophosphatidyl-choline, which is proinflammatory and is considered to be atherogenic. Thus, Lp-PLA2 represents an interesting link between lipoproteinoxidation and vascular inflammation, which likely explain its strong, independent association with CHD risk. Data from the recent ARIC study have indicated that the predictive power of Lp-PLA2 is attenuated after adjustment for multiple established risk factors, but remains strong in the sub-group with LDLcholesterol levels of < 130 mg/dL. Moreover, this study indicated that there was an interaction between Lp-PLA2 and CRP levels, and the strongest predictor for CHD risk (hazard ratio = 2.95) was found for the combination of high CRP (> 3 mg/L) and high Lp-PLA2 (= 422 ug/L) in the presence of LDL < 130 mg/dL. Therefore, CRP and Lp- PLA2 appear to be particularly useful in predicting CHD risk in the presence of LDL cholesterol in the normal range.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lp-PLA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lp-PLA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Lp-PLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Lp-PLA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Lp-PLA2 elisa kit    Lp-PLA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS704213. Click to request current manual
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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