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MMP2 elisa kit :: Rat matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705039
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $450 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
 
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 Product Name   

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) (MMP2), ELISA Kit

★Popular Item★
 Also Known As   

Rat matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

 Product Synonym Names    Rat matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase)
 Product Gene Name   

MMP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705039 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P50757
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat MMP2. No significant cross-reactivity or interference between Rat MMP2 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Detection Range    0.625 ng/ml-40 ng/ml
 Sensitivity    0.156 ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Angiogenesis
 Protein Biological Process 3    Angiogenesis
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP2 purchase    MBS705039 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) (MMP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing MMP2, with an estimated sensitivity of 0.156 ng/ml. The ELISA analytical biochemical technique of the MBS705039 kit is based on MMP2 antibody-MMP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP2 elisa kit

   Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MMP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MMP2 elisa kit    MMP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705039. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP2. It may not necessarily be applicable to this product.
 NCBI GI #    126722977
 NCBI GeneID    100009000
 NCBI Accession #    NP_001075678.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001082209.1 [Other Products]
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 UniProt Primary Accession #    P50757 [Other Products]
 UniProt Related Accession #    P50757 [Other Products]
 Molecular Weight    73,803 Da
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 NCBI Official Full Name    72 kDa type IV collagenase
 NCBI Official Symbol    MMP2 [Similar Products]
 NCBI Protein Information    72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase-2
 UniProt Protein Name    72 kDa type IV collagenase
 UniProt Synonym Protein Names   
72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2
 Protein Family    Matrix metalloproteinase
 UniProt Gene Name    MMP2 [Similar Products]
 UniProt Synonym Gene Names    MMP-2 [Similar Products]
 UniProt Entry Name    MMP2_RABIT
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 UniProt Comments for MMP2    Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues

Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Cofactor: Binds 4 calcium ions per subunit
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 Research Articles on MMP2    1. In conclusion, MMP-2 could be responsible for the proteolysis of dystrophin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP2 elisa kitPathways associated with MMP2 elisa kit
 Reference Product  PubMed Publications
 VEGF elisa kit  >292 publications with MMP2 and VEGF
 TIMP2 elisa kit  >274 publications with MMP2 and TIMP2
 TIMP1 elisa kit  >250 publications with MMP2 and TIMP1
 MMP14 elisa kit  >139 publications with MMP2 and MMP14
 TNF elisa kit  >122 publications with MMP2 and TNF
 TIMP3 elisa kit  >72 publications with MMP2 and TIMP3
 CTGF elisa kit  >32 publications with MMP2 and CTGF
 eNOS elisa kit  >18 publications with MMP2 and eNOS
 CRP elisa kit  >10 publications with MMP2 and CRP
 DCN elisa kit  >6 publications with MMP2 and DCN
 Products by Pathway  Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 GnRH Signaling Pathway antibodies  GnRH Signaling Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
Diseases associated with MMP2 elisa kitOrgans/Tissues associated with MMP2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasm Invasiveness Antibodies  >511 publications with MMP2 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >401 publications with MMP2 and Neoplasm Metastasis
 Inflammation Antibodies  >296 publications with MMP2 and Inflammation
 Disease Progression Antibodies  >200 publications with MMP2 and Disease Progression
 Heart Diseases Antibodies  >187 publications with MMP2 and Heart Diseases
 Necrosis Antibodies  >181 publications with MMP2 and Necrosis
 Breast Neoplasms Antibodies  >162 publications with MMP2 and Breast Neoplasms
 Bone Diseases Antibodies  >103 publications with MMP2 and Bone Diseases
 Colorectal Neoplasms Antibodies  >95 publications with MMP2 and Colorectal Neoplasms
 Aortic Diseases Antibodies  >93 publications with MMP2 and Aortic Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >221 publications with MMP2 and Muscle
 Lung Antibodies  >177 publications with MMP2 and Lung
 Artery Antibodies  >160 publications with MMP2 and Artery
 Heart Antibodies  >135 publications with MMP2 and Heart
 Brain Antibodies  >122 publications with MMP2 and Brain
 Connective Tissue Antibodies  >85 publications with MMP2 and Connective Tissue
 Embryonic Tissue Antibodies  >77 publications with MMP2 and Embryonic Tissue
 Eye Antibodies  >59 publications with MMP2 and Eye
 Prostate Antibodies  >50 publications with MMP2 and Prostate
 Placenta Antibodies  >43 publications with MMP2 and Placenta
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