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MS elisa kit :: Rat melatonin sulfate (MS) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS260007
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

melatonin sulfate (MS), ELISA Kit

 Also Known As   

Rat melatonin sulfate (MS) ELISA Kit

 Product Gene Name   

MS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603174
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 Species Reactivity    Rat
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 Specificity    No cross-reaction with other factors.
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 Samples    Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MS concentration
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78 ng/ml
 Sensitivity    0.1 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MS purchase    MBS260007 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the melatonin sulfate (MS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78 ng/ml in biological research samples containing MS, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS260007 kit is based on MS antibody-MS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MS concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MS elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat MS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of MS elisa kit    MS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS260007. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MS. It may not necessarily be applicable to this product.
 NCBI GI #    2842762
 NCBI GeneID    4548
 NCBI Accession #    Q99707.2 [Other Products]
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 UniProt Secondary Accession #    Q99713; Q99723; A1L4N8; A9Z1W4; B9EGF7 [Other Products]
 UniProt Related Accession #    Q99707 [Other Products]
 Molecular Weight    140,527 Da
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 NCBI Official Full Name    Methionine synthase
 NCBI Official Synonym Full Names    5-methyltetrahydrofolate-homocysteine methyltransferase
 NCBI Official Symbol    MTR [Similar Products]
 NCBI Official Synonym Symbols   
MS; HMAG; cblG
[Similar Products]
 NCBI Protein Information    methionine synthase; cobalamin-dependent methionine synthase; vitamin-B12 dependent methionine synthase; 5-methyltetrahydrofolate-homocysteine methyltransferase 1
 UniProt Protein Name    Methionine synthase
 UniProt Synonym Protein Names   
5-methyltetrahydrofolate--homocysteine methyltransferase; Vitamin-B12 dependent methionine synthase; MS
 UniProt Gene Name    MTR [Similar Products]
 UniProt Synonym Gene Names    MS [Similar Products]
 UniProt Entry Name    METH_HUMAN
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 NCBI Summary for MS    This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
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 UniProt Comments for MS    MTR: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. Defects in MTR are the cause of methylcobalamin deficiency type G (cblG); also known as homocystinuria-megaloblastic anemia complementation type G. It is an autosomal recessive inherited disease that causes mental retardation, macrocytic anemia, and homocystinuria. Mild deficiency in MS activity could be associated with mild hyperhomocysteinemia, a risk factor for cardiovascular disease and possibly neural tube defects. MS mutations could also be involved in tumorigenesis. Defects in MTR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTR may affect the risk of spina bifida via the maternal rather than the embryonic genotype. Belongs to the vitamin-B12 dependent methionine synthase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.13; Methyltransferase; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; cytosol

Molecular Function: homocysteine S-methyltransferase activity; protein binding; zinc ion binding; cobalamin binding; methionine synthase activity

Biological Process: methylation; nervous system development; vitamin metabolic process; methionine biosynthetic process; sulfur amino acid metabolic process; cobalamin metabolic process; xenobiotic metabolic process; pteridine and derivative metabolic process; water-soluble vitamin metabolic process

Disease: Homocystinuria-megaloblastic Anemia, Cblg Complementation Type; Neural Tube Defects, Folate-sensitive
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 Research Articles on MS    1. Trend analyses also revealed the marginally significant lower risk of chronic kidney disease with increasing number of MTR A2756G G allele.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MS elisa kitPathways associated with MS elisa kit
 Reference Product  PubMed Publications
 MTHFR elisa kit  >264 publications with MS and MTHFR
 CBS elisa kit  >76 publications with MS and CBS
 MTHFD1 elisa kit  >44 publications with MS and MTHFD1
 SHMT1 elisa kit  >37 publications with MS and SHMT1
 DHFR elisa kit  >21 publications with MS and DHFR
 CTH elisa kit  >5 publications with MS and CTH
 AHCY elisa kit  >4 publications with MS and AHCY
 MAT2A elisa kit  >2 publications with MS and MAT2A
 SHMT2 elisa kit  >1 publications with MS and SHMT2
 MAT1A elisa kit  >1 publications with MS and MAT1A
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies  Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
 Defective BTD Causes Biotidinase Deficiency Pathway antibodies  Defective BTD Causes Biotidinase Deficiency Pathway Diagram
 Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies  Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
 Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Diseases associated with MS elisa kitOrgans/Tissues associated with MS elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >462 publications with MS and Nervous System Diseases
 Demyelinating Diseases Antibodies  >242 publications with MS and Demyelinating Diseases
 Brain Diseases Antibodies  >214 publications with MS and Brain Diseases
 Central Nervous System Diseases Antibodies  >203 publications with MS and Central Nervous System Diseases
 Cardiovascular Diseases Antibodies  >98 publications with MS and Cardiovascular Diseases
 Atrophy Antibodies  >74 publications with MS and Atrophy
 Gastrointestinal Diseases Antibodies  >51 publications with MS and Gastrointestinal Diseases
 Dementia Antibodies  >42 publications with MS and Dementia
 Disease Models, Animal Antibodies  >40 publications with MS and Disease Models, Animal
 Cognition Disorders Antibodies  >39 publications with MS and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >501 publications with MS and Brain
 Blood Antibodies  >204 publications with MS and Blood
 Nerve Antibodies  >131 publications with MS and Nerve
 Bone Antibodies  >54 publications with MS and Bone
 Liver Antibodies  >54 publications with MS and Liver
 Muscle Antibodies  >48 publications with MS and Muscle
 Vascular Antibodies  >48 publications with MS and Vascular
 Heart Antibodies  >36 publications with MS and Heart
 Lung Antibodies  >29 publications with MS and Lung
 Kidney Antibodies  >27 publications with MS and Kidney
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