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NTF4 elisa kit :: Rat neurotrophin 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS700037
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,495 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,715 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

neurotrophin 4 (NTF4), ELISA Kit

★Popular Item★
 Also Known As   

Rat Neurotrophin 4, NT-4 ELISA Kit

 Product Synonym Names    Rat Neurotrophin 4; NT-4 ELISA KIT; GLC10; GLC1O; NT-4/5; NT4; NT5; NTF5; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5; neurotrophin 5 (neurotrophin 4/5) ; neurotrophin 4
 Product Gene Name   

NTF4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS700037 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P34131
 Species Reactivity    Rat
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Rat NTF4. No significant cross-reactivity or interference between Rat NTF4 and analogues was observed.
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 Samples    Serum, plasma and tissue homogenates
 Detection Range    47 pg/ml-3000 pg/ml
 Sensitivity    11.75 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NTF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NTF4 purchase    MBS700037 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neurotrophin 4 (NTF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 47 pg/ml-3000 pg/ml in biological research samples containing NTF4, with an estimated sensitivity of 11.75 pg/ml. The ELISA analytical biochemical technique of the MBS700037 kit is based on NTF4 antibody-NTF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NTF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NTF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NTF4 elisa kit

   Introduction: Neurotrophins are a family of proteins that induce the survival, development and function of neurons. They belong to a class of growth factors, secreted proteins, which are capable of signaling particular cells to survive, differentiate, or grow. Growth factors such as neurotrophins that promote the survival of neurons are known as neurotrophic factors. Neurotrophic factors are secreted by target tissue and act by preventing the associated neuron from initiating programmed cell death - thus allowing the neurons to survive. Neurotrophins also induce differentiation of progenitor cells, to form neurons. Neurotrophin-4 (NT-4) like BDNF, is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NT-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NT-4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NT-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NT-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of NTF4 elisa kit    NTF4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS700037. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NTF4. It may not necessarily be applicable to this product.
 NCBI GI #    31377533
 NCBI GeneID    25730
 NCBI Accession #    NP_037316.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013184.3 [Other Products]
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 UniProt Primary Accession #    P34131 [Other Products]
 UniProt Related Accession #    P34131 [Other Products]
 Molecular Weight    22,333 Da
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 NCBI Official Full Name    neurotrophin-4
 NCBI Official Synonym Full Names    neurotrophin 4
 NCBI Official Symbol    Ntf4 [Similar Products]
 NCBI Official Synonym Symbols   
NT4P; Ntf5
[Similar Products]
 NCBI Protein Information    neurotrophin-4; NT-4; NT-5; neurotrophin-5; neutrophic factor 4; Neurotrophin 5 (neurotrophin 4/5)
 UniProt Protein Name    Neurotrophin-4
 UniProt Synonym Protein Names   
Neurotrophin-5; NT-5; Neutrophic factor 4
 Protein Family    Neurotrophin
 UniProt Gene Name    Ntf4 [Similar Products]
 UniProt Synonym Gene Names    Nt4; Ntf5; NT-4; NT-5 [Similar Products]
 UniProt Entry Name    NTF4_RAT
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 NCBI Summary for NTF4    promotes the survival of peripheral sensory and sympathetic neurons [RGD, Feb 2006]
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 UniProt Comments for NTF4    Function: Could serve as a target-derived trophic factor for sensory and sympathetic neurons.
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 Research Articles on NTF4    1. In a permanent middle cerebral artery occlusion model, ischemia decreased NT-4 and trkB expression. Immunohistochemistry showed their immunoreactivities around the region of the ischemic area.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with NTF4 elisa kitPathways associated with NTF4 elisa kit
 Reference Product  PubMed Publications
 Ntf3 elisa kit  >10 publications with NTF4 and Ntf3
 Ntrk2 elisa kit  >7 publications with NTF4 and Ntrk2
 Ntrk1 elisa kit  >4 publications with NTF4 and Ntrk1
 Ngfr elisa kit  >3 publications with NTF4 and Ngfr
 Ntrk3 elisa kit  >2 publications with NTF4 and Ntrk3
 Products by Pathway  Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Neurotrophin Signaling Pathway antibodies  Neurotrophin Signaling Pathway Diagram
 Neurotrophin Signaling Pathway antibodies  Neurotrophin Signaling Pathway Diagram
Diseases associated with NTF4 elisa kitOrgans/Tissues associated with NTF4 elisa kit
 Disease Name  Pubmed Publications
 Nerve Degeneration Antibodies  >2 publications with NTF4 and Nerve Degeneration
 Carcinoma Antibodies  >1 publications with NTF4 and Carcinoma
 Nervous System Diseases Antibodies  >1 publications with NTF4 and Nervous System Diseases
 Hypersensitivity Antibodies  >1 publications with NTF4 and Hypersensitivity
 Lung Diseases Antibodies  >1 publications with NTF4 and Lung Diseases
 Leukemia Antibodies  >1 publications with NTF4 and Leukemia
 Inflammation Antibodies  >1 publications with NTF4 and Inflammation
 Neoplasm Metastasis Antibodies  >1 publications with NTF4 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >1 publications with NTF4 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with NTF4 and Brain
 Mouth Antibodies  >2 publications with NTF4 and Mouth
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